Results 151 to 160 of about 170,154 (204)

Assay optimization for the objective quantification of human multilineage colony-forming units

Experimental Hematology, 2023
Colony-forming unit (CFU) assays are a powerful tool in hematopoietic research because they allow researchers to functionally test the lineage potential of individual stem and progenitor cells. Assaying for lineage potential is important for determining and validating the identity of progenitor populations isolated by methods such as fluorescence ...
Evrett N. Thompson, Maximillian J. Carlino, Vanessa M. Scanlon, H. Leighton Grimes, Diane S. Krause   +4 more
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Colony Forming Unit Assays

2016
Mesenchymal stem/stromal cells (MSCs) have been extensively investigated for their potential to regenerate tissue, to modulate the immune system, and their wound healing properties in over 350 clinical trials worldwide. MSCs from various tissues such as adipose, bone, and others are currently being studied in clinical trials in indications for ischemic,
Patrice, Penfornis, Radhika, Pochampally
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Colony-Forming Unit Cell (CFU-C) Assays in Myelodysplastic Syndrome

Blood, 2015
Abstract Introduction: The myelodysplastic syndromes (MDS) are a group of clonal diseases derived from hematopoietic stem cells (HSC). Colony-forming unit cell (CFU-C) assay is an effective method to study the number and the function of HSC in vitro.
Bing Li, Jinqin Liu, Shiqiang Qu, Robert Peter Gale, Ruixian Xing, Tiejun Qin, Yue Zhang, Zefeng Xu, Liwei Fang, Hongli Zhang, Lijuan Pan, Naibo Hu, Gang Huang, Zhijian Xiao   +13 more
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Variability in subjective review of umbilical cord blood colony forming unit assay

Cytometry Part B: Clinical Cytometry, 2016
BackgroundThe goal of this study was to quantitatively evaluate the reproducibility of current manual counting methods of colony forming units (CFUs) from umbilical cord blood samplesMethodsFresh and reconstituted frozen cells from 10 cord blood samples were cultured under standard conditions.
K, Powell, E, Kwee, B, Nutter, E, Herderick, P, Paul, D, Thut, C, Boehm, G, Muschler   +7 more
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Bone Marrow Colony-Forming Unit Assay in Cats With Naturally Occurring Myelodysplastic Syndromes

International Journal of Hematology, 2001
Feline myelodysplastic syndromes (MDS) has been diagnosed in many cats infected with feline leukemia virus, although the pathogenesis of this hematopoietic deficiency has been unclear. In this study, we assayed the bone marrow erythroid colony-forming units (CFU-E) and granulocyte-machrophage CFUs (CFU-GM) to investigate the pathogenesis of feline MDS.
Naho, Nagashima, Masaharu, Hisasue, Katsumi, Higashi, Rui, Kano, Hajime, Tsujimoto, Atsuhiko, Hasegawa   +5 more
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Rat granulocyte colony-forming unit (CFU-G) assay for the assessment of drug-induced hematotoxicity

Toxicology in Vitro, 2002
To assess the drug-induced hematotoxicity to granulocyte progenitors, we established a modified colony-forming assay using rat bone marrow cells (BMCs). In the presence of various colony-stimulating factors (CSFs), rat BMCs were disseminated on methylcellulose at a concentration of 1.3 x 10(4) cells/cm(2) (5 x 10(4) cells/0.5 ml/well in a 12-well plate)
K, Matsumura-Takeda, K, Kotosai, A, Ozaki, H, Hara, S, Yamashita   +4 more
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Aldehyde dehydrogenase–bright cells correlated with the colony‐forming unit–granulocyte‐macrophage assay of thawed cord blood units

Transfusion, 2014
BackgroundThe number of aldehyde dehydrogenase–bright (ALDHbr) cells has been suggested as a viable marker of hematopoietic stem cell function. We evaluated the suitability of ALDHbr cell analysis in the quality assessment of postthaw cord blood (CB) units.Study Design and MethodsA total of 245 CB units were obtained for estimating the numbers of total
Hye Ryun Lee, Sue Shin, Jong Hyun Yoon, Eun Youn Roh, Byoung Jae Kim, Eun Young Song   +5 more
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Colony Forming Unit Assays for MSCs

2008
MSCs are the plastic adherent adult stem/progenitor cells from bone marrow originally referred to as fibroblastoid colony forming units, then in the hematological literature as marrow stromal cells, subsequently as mesenchymal stem cells, and most recently as multipotential mesenchymal stromal cells.
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