Results 161 to 170 of about 170,154 (204)
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Toward refinement of the colony-forming unit-granulocyte/macrophage clonogenic assay: Inclusion of a metabolic system

Toxicology in Vitro, 2006
This work represents a first attempt to refine the colony-forming unit-granulocyte/macrophage (CFU-GM) clonogenic assay by incorporating liver microsomes and co-factors as a metabolic system into the in vitro test system in response to an ECVAM recommendation.
Gianni, Dal Negro   +3 more
openaire   +2 more sources

Candida albicans viability after exposure to amphotericin B: Assessment using metabolic assays and colony forming units

Journal of Microbiological Methods, 2008
Metabolic assays are a preferred method for evaluation of Candida albicans viability after exposure to antimicrobial agents in cases in which the culture is a complex mixture of yeast and filamentous forms. There is a lack of published data indicating the strength of the correlation between metabolic assays and viable cell numbers determined by a ...
Prasanna D, Khot   +2 more
openaire   +2 more sources

Prevalidation of a model for predicting acute neutropenia by colony forming unit granulocyte/macrophage (CFU-GM) assay

Toxicology in Vitro, 2001
This report describes an international prevalidation study conducted to optimise the Standard Operating Procedure (SOP) for detecting myelosuppressive agents by CFU-GM assay and to study a model for predicting (by means of this in vitro hematopoietic assay) the acute xenobiotic exposure levels that cause maximum tolerated decreases in absolute ...
A. Pessina   +12 more
openaire   +5 more sources

Refinement of the colony-forming unit-megakaryocyte (CFU–MK) assay for its application to pharmaco-toxicological testing

Toxicology in Vitro, 2003
In vitro colony-forming unit (CFU) assays can be used in the evaluation of potentially haematotoxic compounds during preclinical testing. The use of undifferentiated haematopoietic cells, able to proliferate and commit towards a specific blood cell lineage, enable selective toxicity to be detected.
S, Casati   +3 more
openaire   +2 more sources

The Granulocyte/Macrophage Colony-Forming Unit Assay

1998
Although the complex dynamics of the interactions among the different components of the hematopoietic system remains poorly understood, much progress has been made in identifying the various cell types and the multitude of growth factors regulating its homeostasis.
openaire   +1 more source

A Single-Step Colony-Forming Unit Assay for Unseparated Mobilized Peripheral Blood, Cord Blood, and Bone Marrow

Journal of Hematotherapy & Stem Cell Research, 2001
The colony-forming unit (CFU) assay is exposed to a lot of variation, part of which is introduced by several enrichment strategies that are routinely performed before assessment of clonogenic capacity in mobilized peripheral blood (PB), bone marrow (BM), or cord blood (CB).
A M, de Kreuk   +5 more
openaire   +2 more sources

Radiosensitivity of Murine Hemopoietic Colony-Forming Units Assayed in Situ in the Rib and in Other Marrow Sites

Radiation Research, 1986
The radiosensitivity of murine hemopoietic colony-forming cells, which produce colonies in situ and which were counted at Day 8 after irradiation in sections of the femur, humerus, sternum, and spleen, is characterized by a D0 value of 91 +/- 9 cGy. The radiosensitivity of such cells in the rib was assessed using a new technique measuring regeneration ...
J H, Hendry, R, Schofield, N E, Bwire
openaire   +2 more sources

Transition metal catalyzed mineralization of Calcon and bioassay of the mineralized solutions by Escherichia coli colony forming unit assay

Chemical Engineering Journal, 2012
Mineralization of Calcon was studied by Fenton and Fenton like processes in a wide range of pH with hydrogen peroxide (HP) and ammonium persulphate (APS) as oxidants and catalyzed by transition metal catalysts such as Fe2+, Ag+ and Co2+. Maximum mineralization for Fenton and other processes was achieved at pH 3 and 1, respectively.
M.K. Sahoo   +4 more
openaire   +1 more source

Quantification of Fusarium solani f. sp. glycines isolates in soybean roots by colony-forming unit assays and real-time quantitative PCR

Theoretical and Applied Genetics, 2008
Fusarium solani f. sp. glycines (FSG; syn. F. virguliforme Akoi, O'Donnell, Homma & Lattanzi) is a soil-borne fungus that infects soybean roots and causes sudden death syndrome (SDS), a widespread and destructive soybean disease. The goal of this study was to develop and use a real-time quantitative polymerase chain reaction (QPCR) assay to compare the
S, Li   +3 more
openaire   +2 more sources

Refinement and verification of test conditions for colony-forming unit assay in rat bone marrow cells with preservable colony specimens

Comparative Clinical Pathology, 2012
We established test conditions for colony-forming unit assay using rat bone marrow cells in which the colony specimens can be preserved. In our test system, all colonies can be transferred from a petri dish to a slide glass with whole agar medium because the agar medium volume is small and movable, enabling the agar medium to be dried and then fixed on
Kazuhiko Takamatsu   +3 more
openaire   +1 more source

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