Cryopreserved bone marrow aspirate concentrate as a cell source for the colony-forming unit fibroblast assay [PDF]
Cytotherapy, 2020 The prevalence of connective tissue progenitor cells within a cell-based therapy is often quantified using the colony-forming unit fibroblast (CFU-F) assay. The present study investigates the feasibility of using cryopreserved bone marrow aspirate concentrate (BMAC) as an alternative cell source to fresh BMAC for CFU-F quantification.Freshly prepared ...
Dustin R, Berger +3 more
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Bio-Protocol, 2016 We performed a growth inhibition assay to test antibacterial compounds in leaf extracts from transgenic rice plants. The assay is based on over-night co-incubation of a defined concentration of colony forming units (cfu) of the respective bacteria ...
Marco Loehrer +3 more
doaj +1 more source
The Efficiency of the Assay for Haemopietic Colony Forming Cells [PDF]
, 1970 The quantitative efficiency of the spleen colony assay in mice is discussed in the light of recent findings on the kinetics of colony forming cells. Arguments are presented showing that the f factor, the 2 hr CFU recovery fraction in the spleen, markedly
Keizer, H.J. +2 more
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Frontiers in Veterinary Science, 2022 Haematopoietic stem and progenitor cells (HSPCs) are used for transplantation to reconstruct the haematopoietic pathways in humans receiving severe chemotherapy. However, the characteristics of canine HSPCs, such as specific surface antigens and gene expression profiles, are still unclear. This study aimed to characterise the haematopoietic ability and
Taro Ayabe +9 more
openaire +3 more sources
Mechanisms of growth inhibition of primary prostate epithelial cells following gamma irradiation or photodynamic therapy including senscence, necrosis, and autophagy, but not apoptosis [PDF]
, 2015 In comparison to more differentiated cells, prostate cancer stem-like cells are radioresistant, which could explain radio-recurrent prostate cancer. Improvement of radiotherapeutic efficacy may therefore require combination therapy.
Boyle, Ross W. +7 more
core +1 more source
Disruption of HOX activity leads to cell death that can be enhanced by the interference of iron uptake in malignant B cells. [PDF]
, 2010 The HOX genes encode a family of transcription factors that are dysregulated in several malignancies and have been implicated in oncogenesis and cancer cell survival.
Daniels, TR +5 more
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Antibiotics, 2021 Bacterial inactivation using bacteriophages (or phages) has emerged as an effective solution for bacterial infections, but the screening methods used to evaluate the effectiveness of the phages to inactivate bacteria are not fast, reliable or precise ...
Pedro Costa +4 more
doaj +1 more source
A quantitative assay for transformation of bone marrow cells by Abelson murine leukemia virus [PDF]
, 1976 A quantitative Abelson murine leukemia virus (A-MuLV) lymphoid cell transformation assay has been developed using a semisolid agarose culture system. Under these conditions lymphoid cell transformation was shown to vary linearly with the dose of A-MuLV ...
Baltimore, David, Rosenberg, Naomi
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Histone deacetylase 3 gene in hematopoietic stem cells promotes mouse lymphogenesis
Di-san junyi daxue xuebao, 2019 Objective To investigate the role of histone deacetylase 3 gene (Hdac3) in hematopoiesis by developing a conditional Hdac3 knockout mice. Methods We crossed the Hdac3floxp/floxp mice with Mx1-Cre mice to generate Hdac3fl/flMx1-Cre mice, and then ...
LYU Wenqiong +3 more
doaj +1 more source
Development of a standardized and safe airborne antibacterial assay, and its evaluation on antibacterial biomimetic model surfaces. [PDF]
PLoS ONE, 2014 Bacterial infection of biomaterials is a major concern in medicine, and different kinds of antimicrobial biomaterial have been developed to deal with this problem.
Ali Al-Ahmad +5 more
doaj +1 more source