Polyhexamethylene Biguanide and Nadifloxacin Self-Assembled Nanoparticles: Antimicrobial Effects against Intracellular Methicillin-Resistant Staphylococcus aureus [PDF]
, 2018 The treatment of skin and soft tissue infections caused by methicillin-resistant Staphylococcus aureus (MRSA) remains a challenge, partly due to localization of the bacteria inside the host’s cells, where antimicrobial penetration and efficacy is limited.
Chivu, A +3 more
core +3 more sources
Increasing the High Throughput of a Luminescence-Based Serum Bactericidal Assay (L-SBA)
BioTech, 2021 Serum bactericidal assay (SBA) is the method to investigate in vitro complement-mediated bactericidal activity of sera raised upon vaccination. The assay is based on incubating the target bacteria and exogenous complement with sera at different dilutions
Maria Grazia Aruta +4 more
doaj +1 more source
Molecules, 2022 Polymyxin-based combination therapy is commonly used to treat carbapenem-resistant Acinetobacter baumannii (CRAB) infections. In the present study, the bactericidal effect of polymyxin B and minocycline combination was tested in three CRAB strains ...
Xingyi Qu +12 more
doaj +1 more source
Expression of NanoLuc Luciferase in Listeria innocua for Development of Biofilm Assay
Frontiers in Microbiology, 2021 Studies of biofilm formation by bacteria are crucial for understanding bacterial resistance and for development of novel antibacterial strategies. We have developed a new bioluminescence biofilm assay for Listeria innocua, which is considered a non ...
Aleš Berlec +5 more
doaj +1 more source
TIV vaccination modulates host responses to influenza virus infection that correlate with protection against bacterial superinfection [PDF]
, 2019 Background: Influenza virus infection predisposes to secondary bacterial pneumonia. Currently licensed influenza vaccines aim at the induction of neutralizing antibodies and are less effective if the induction of neutralizing antibodies is low and/or the
Choi, Angela +4 more
core +2 more sources
BMC Microbiology, 2019 Background Quantification of intracellular bacteria is fundamental in many areas of cellular and clinical microbiology to study acute and chronic infections.
Meysam Sarshar +8 more
doaj +1 more source
Comparison between adenosine triphosphate bioluminescence and aerobic colony count to assess surface sanitation in the hospital environment [PDF]
, 2018 Background: Adenosine triphosphate bioluminescence produced by the firefly luciferase has been successfully introduced to verify cleaning procedures in the food industry according to the Hazard Analysis Critical Control Point program.
Alagna E. +8 more
core +1 more source
Development of a high-throughput method to evaluate serum bactericidal activity using bacterial ATP measurement as survival readout. [PDF]
PLoS ONE, 2017 Serum Bactericidal Activity (SBA) assay is the method of choice to evaluate the complement-mediated functional activity of both infection- and vaccine-induced antibodies.
Francesca Necchi +2 more
doaj +1 more source
Preinfection in vitro chemotaxis, phagocytosis, oxidative burst, and expression of CD11/CD18 receptors and their predictive capacity on the outcome of mastitis induced in dairy cows with Escherichia coli. [PDF]
, 1997 Four to 6 wk after parturition, 12 cows in second, fourth, or fifth lactation were experimentally infected in one gland with Escherichia coli. The capacity of chemotaxis, phagocytosis, oxidative burst, and expression of CD11/CD18 receptors to predict the
A. Brand +30 more
core +2 more sources
BMC Research Notes, 2012 Background The bacterium Staphylococcus aureus constitutes one of the most important causes of nosocomial infections. One out of every three individuals naturally carries S.
Frech Georges C +3 more
doaj +1 more source