Results 61 to 70 of about 941,860 (298)

DNA ligase LigE increases bacterial transformation with damaged extracellular DNA

FEBS Letters, EarlyView.
The DNA ligase LigE from the naturally competent Gram‐negative bacterium Neisseria gonorrhoaea increases transformation with cut extracellular DNA. This process is ATP‐dependent and relies on the catalytic activity of LigE; it also requires that LigE contains an intact periplasmic localisation sequence, indicating that ligE functions in the periplasm ...
Jolyn Pan, Avi Singh, Shrawani Weralupe Gamladdalage, Joanna Hicks, Adele Williamson   +4 more
wiley   +1 more source

Comparison between adenosine triphosphate bioluminescence and aerobic colony count to assess surface sanitation in the hospital environment

Epidemiology, Biostatistics and Public Health, 2022
Background: Adenosine triphosphate bioluminescence produced by the firefly luciferase has been successfully introduced to verify cleaning procedures in the food industry according to the Hazard Analysis Critical Control Point program.
Daniele Domenico Raia, Lucia Cannova, Sandro Provenzano, Omar Enzo Santangelo, Dario Piazza, Enrico Alagna, Valentina Bonanno, Luigi Aprea, Alberto Firenze   +8 more
doaj   +1 more source

Loss of IGF‐1R impairs DNA‐PKcs recruitment to chromatin leading to defective end‐joining

Molecular Oncology, EarlyView.
IGF‐1R promotes radioresistance by facilitating DNA‐PKcs recruitment to chromatin, enabling non‐homologous end‐joining (NHEJ) repair of double‐strand breaks. Inhibition or loss of IGF‐1R disrupts this recruitment to damage sites, driving compensatory reliance on microhomology‐mediated end‐joining (MMEJ) repair.
Matthew O. Ellis, Jack V. Mills, Wojciech Niedzwiedz, Valentine M. Macaulay   +3 more
wiley   +1 more source

Changes in quality of Liaoning Zhuanghe Dagu chicken jerky at different storage temperatures and establishment of shelf life prediction model

Shipin yu jixie
ObjectiveTo investigate the changes in the quality of Dagu chicken jerky during storage at different temperatures and establish a shelf-life prediction model based on thiobarbituric acid reactive substance (TBARS) value and colony count.MethodsThe Dagu ...
JIN Na, YAN Ruichi, SU Yuhong, WANG Xizhu, SHANG Hongli   +4 more
doaj   +1 more source

Mechanical bowel preparation does not affect the intramucosal bacterial colony count

, 2010
PURPOSE: The aim of this study was to determine if mechanical bowel preparation (MBP) influences the intramucosal bacterial colony count in the colon. MATERIALS AND METHODS: Macroscopically normal colon mucosa was collected from 37 patients (20 with and ...
Jung, Bärbel,, Nilsson, Erik,, Ransjö, Ulrika, Påhlman, Lars, Smedh, Kenneth, Ransjö, Ulrika,, Påhlman, Lars,, Nilsson, Erik, Matthiessen, Peter   +8 more
core   +1 more source

USP29‐regulated noncanonical stabilization of the hypoxia‐inducible factor‐α in aggressive prostate cancer

Molecular Oncology, EarlyView.
We identify USP29 as the only DUB mirroring CA9 expression, a marker of hypoxia and HIF pathway activation associated with PCA aggressiveness. USP29 stabilizes HIF‐1α and HIF‐2α via a noncanonical mechanism that is independent of PHD/pVHL activity yet relies on proteasomal regulation, establishing USP29 as a previously unrecognized regulator of hypoxic
Amelie S Schober, Leire Moreno‐Cugnon, Laura Martínez‐Pérez, Amaia Ercilla, Amaia Zabala‐Letona, Adrián Vicente‐Barrueco, Maider Fagoaga‐Eugui, Saioa Garcia‐Longarte, Blanca Calle‐Ciborro, Encarnación Pérez‐Andrés, Onintza Carlevaris, Sara Pozo, Isabel Mendizabal, Ugo Mayor, Arkaitz Carracedo, Violaine Sée, Edurne Berra   +16 more
wiley   +1 more source

Automatic Programming with Ant Colony Optimization [PDF]

, 2004
Automatic programming is the use of search techniques to find programs that solve a problem. The most commonly explored automatic programming technique is genetic programming, which uses genetic algorithms to carry out the search.
Green, Jennifer, Johnson, Colin G., Whalley, Jacqueline L.   +2 more
core  

Percentage cell reduction curves based on colony count method vs. XTT assay.

, 2013
Percentage cell reduction curves of P. aeruginosa biofilm cells upon exposure to the 20 kHz plasma jet. The dotted line is based on the standard colony count method whereas the solid line is based on the XTT assay.
Garry Laverty (309200), Mahmoud Y. Alkawareek (309198), Qais Th. Algwari (309199), Deborah O'Connell (309203), Sean P. Gorman (309201), Brendan F. Gilmore (309204), William G. Graham (309202)   +6 more
core   +1 more source

Finding novel vulnerabilities of hypomorphic BRCA1 alleles

Molecular Oncology, EarlyView.
Synthetic lethality screens performed to identify novel vulnerabilities often model complete gene loss, thereby overlooking patient‐derived hypomorphic mutations. In this study, we have performed genome‐wide CRISPR screens on BRCA1 hypomorphic mutations, showing BRCA1I26A behaves like wild‐type, while BRCA1R1699Q mimics deficiency. Furthermore, we have
Anne Schreuder, Klaas de Lint, Hồ Mỹ Phúc Võ, Mariana M. Góis, Rosalie A. Kampen, Marta San Martin Alonso, Ilse Nootenboom, Veronica Garzero, Tiemen J. Wendel, Rob M. F. Wolthuis, Sylvie M. Noordermeer   +10 more
wiley   +1 more source

In vitro and in silico modelling of ROS1‐positive non‐small cell lung cancer reveals fusion‐dependent tyrosine kinase inhibitor responses

Molecular Oncology, EarlyView.
Drug resistance limits treatment success in a subset of lung cancers driven by ROS1 gene alterations. Using patient‐derived cells and computer simulations, we studied three key mutations and how they affect five targeted drugs. The mutations reduced drug effectiveness in different ways by altering protein structure and behavior.
Farhan Ul Haq, Marc Terrones, Felicia Rodrigues Fortes, Anne Schepers, Alessia Denis, Christophe Deben, Ken Op de Beeck, Guy Van Camp, Geert Vandeweyer   +8 more
wiley   +1 more source