Results 71 to 80 of about 160,344 (260)

Directed evolution of enzymes at the crossroads of tradition and innovation

FEBS Open Bio, EarlyView.
An iterative cycle of data‐driven enzyme optimization comprising four stages: genetic diversification of a template enzyme, expression of protein variants, high‐throughput evaluation, and machine‐learning‐guided redesign of the next variant library.
Maria Tomkova, Andrej Mirossay, Erik Sedlak   +2 more
wiley   +1 more source

Bacterial Load on the Surface of Amniocentesis Operators with or without Long-Sleeved Surgical Gowns: A Prospective Cohort Study

Clinical and Experimental Obstetrics & Gynecology
Background: There is currently no specific requirement for operator’s attires performing amniocentesis, especially for repetitive amniocenteses. We investigated the contamination status on the surface of operators with or without long-sleeved surgical ...
Jinyu Wang, Ying Song, Lingling Sun, Xijing Liu, Fan Zhou, Qianying Zhao, Jing Wang, Xuemei Zhang, Mei Yang, Dan Xie, Ting Hu, Shanling Liu   +11 more
doaj   +1 more source

Molecular characterization of covRS mutations in M1UK Streptococcus pyogenes

FEBS Open Bio, EarlyView.
Group A Streptococcus (GAS) acquires covRS mutations driving a hypervirulent bacterial state, frequently associated with invasive disease‐like necrotizing fasciitis. We demonstrate that the newly emerged M1UK GAS lineage can also acquire these mutations.
Jarrad Pritchard, Emma‐Jayne Proctor, Jiawa Wang, Nikolas P. Johnston, Andrew Hayes, Georgia McCorkell, Haibo Yu, Jai Tree, Mark R. Davies, Mark J. Walker, Ronald Sluyter, Stephan Brouwer, Martina L. Sanderson‐Smith   +12 more
wiley   +1 more source

SOME STUDIES OF THE COLONY COUNT TECHNIQUE FOR SOIL BACTERIA

Journal of Applied Bacteriology, 1960
SUMMARY: A series of co‐operative experiments was conducted to compare the bacterial colony counts of soil obtained by workers in different laboratories, using soil extract agar and other media for the determinations. In the earlier experiments it was not possible to obtain a
Edgell, J. W., Cuthbert, W. A., Scarlett, C. A., Thomas, S. B., Westmacott, M. H., Bird, E. R., Harrison, J.   +6 more
openaire   +1 more source

Loss of AMBRA1 activates MAPK and angiogenesis signaling pathways in melanoma cells

FEBS Open Bio, EarlyView.
Loss of AMBRA1 in melanoma cells activates multiple oncogenic pathways associated with tumor progression. Transcriptomic and protein network analyses revealed that AMBRA1 depletion enhances MAPK/ERK signaling, angiogenesis, TGF‐β/EMT signaling, and Wnt/axon guidance pathways.
Milad Ibrahim, Marco Corazzari, Iman Osman, Jane Armstrong, Noel Carter   +4 more
wiley   +1 more source

Structural studies and functional engineering of NanX: an anhydro‐sialic acid transporter from Escherichia coli

FEBS Open Bio, EarlyView.
Biophysical characterisation shows that NanX, a membrane transport protein from the major facilitator superfamily (MFS), forms both monomers and dimers after purification. AlphaFold modelling and substrate docking provide information on residues likely involved in substrate recognition for NanX and another MFS member, NanT.
Michael C. Newton‐Vesty, Kelsi R. Hall, Zachary D. Tillett, Liam S. Turk, James S. Davies, Michael J. Currie, Hamish G. Brown, Sepideh Valimehr, Peter D. Mace, Andrew E. Whitten, Santosh Panjikar, Eric Hanssen, Rachel A. North, Renwick C.J. Dobson   +13 more
wiley   +1 more source

Integrated PANoptosis Profiling Identifies Immunosuppressive Subtypes and a Prognostic Signature With Functional Validation of MLKL in Glioblastoma

Annals of Clinical and Translational Neurology, EarlyView.
ABSTRACT Objective The prognosis of glioblastoma (GBM) remains highly unfavorable, largely due to high tumor heterogeneity and an immunosuppressive microenvironment. However, the functional role of PANoptosis in this context is poorly understood. Methods Patients were stratified via K‐means clustering. A risk score model was constructed using prognosis‐
Langfei Tian, Minghui Zhao, Yuanbo Hu, Kaiyue Wang, Haiguang Liu, Zetong Bai, Kebin Zheng   +6 more
wiley   +1 more source

Quantification methods of Candida albicans are independent irrespective of fungal morphology

Microbial Cell
The ability of Candida albicans to switch its morphology from yeast to filaments, known as polymorphism, may bias the methods used in microbial quantification.
Amanda B Soares, Maria C de Albuquerque, Leticia M Rosa, Marlise I Klein, Ana C Pavarina, Paula A Barbugli, Livia N Dovigo, Ewerton G de O Mima   +7 more
doaj   +1 more source

In Vitro Effect of a High-Intensity Laser on Candida albicans Colony Count. [PDF]

J Lasers Med Sci, 2022
Momeni E, Didehdar M, Sarlak E, Safari M.   +3 more
europepmc   +1 more source

A Return to Normality: A Descriptive Qualitative Interview Study Exploring the Patient Experience of Gout Flare Resolution

Arthritis Care &Research, EarlyView.
Objective Although the definition of a gout flare is well established, the state of gout flare resolution has not yet been defined. This study aimed to explore patients’ experiences and perceptions of gout flare resolution. Methods Semistructured interviews were conducted with 24 people with gout, guided by open‐ended questions exploring their ...
Sarah Stewart, Anne Horne, Angelo Gaffo, Lisa K. Stamp, N. Lawrence Edwards, Nicola Dalbeth   +5 more
wiley   +1 more source