Results 141 to 150 of about 2,925,624 (251)

In silico and in vitro exploration of a tyrosinase for biocatalytic production of catechols

FEBS Open Bio, EarlyView.
Tyrosinase from Ralstonia pseudosolanacearum is a promising biocatalyst for producing valuable catechols from monophenol substrates. This tyrosinase is uniquely suited to this due to its high monophenolase : diphenolase ratio. We combined in silico docking and in vivo kinetic characterisation of this tyrosinase with 11 industrially relevant monophenols,
James Britton, Fang Zhao, Reeta Davis, Meg Walsh, Kevin E O'Connor, David O'Connell, Tanja Narancic   +6 more
wiley   +1 more source

Protocol for quantifying miRNA trafficking across the endosomal membrane

FEBS Open Bio, EarlyView.
An in vitro protocol measures miRNA uptake into endosomes isolated from mammalian cell extracts, which are free of subcellular contaminants. Performed at 37 °C in the presence of ATP, it ensures the import of single‐stranded miRNA into the endosomal lumen.
Syamantak Ghosh, Kamalika Mukherjee, Suvendra N. Bhattacharyya   +2 more
wiley   +1 more source

Combined Transcriptomic and Metabolomic Analysis of the Coloration Mechanism in Colored-Leaf <i>Osmanthus fragrans</i> 'Jinyu Guihua'. [PDF]

Plants (Basel)
Guo P, Huang Y, Jin P, Li X, Ma Q, Yu L, Zhao W, Wang Y, Shang F.   +8 more
europepmc   +1 more source

Heterotropic regulation and negative homotropic cooperativity

FEBS Open Bio, EarlyView.
We identified a structural module common to some proteins that couple negative cooperativity with heterotropic regulation, two features that rarely coexist. These proteins are ring‐like and present an ordered asymmetry whereby noncontacting subunits are symmetric, and their tertiary structure differs from that of contacting subunits.
Veronica Morea, Francesco Angelucci, Federica Arnesano, Gianmarco Pascarella, Patrizio Di Micco, Andrea Bellelli   +5 more
wiley   +1 more source

Integrative analysis of the transcriptome and metabolome reveals the peel coloration mechanism of Zanthoxylum armatum. [PDF]

BMC Plant Biol
Ren Y, Liu J, He L, Jiang J, Gong T, Zhou Q, Li Q, Liu J, Tang N, Chen Z.   +9 more
europepmc   +1 more source

BCG vaccination potentiates oxidative phosphorylation in neonatal myeloid‐derived suppressor cells

FEBS Open Bio, EarlyView.
BCG vaccination enhances oxidative phosphorylation in neonatal MDSCs, impairing their immunosuppressive function. It upregulates electron transport chain genes and mitochondrial activity, increasing ATP and oxygen consumption. Pharmacological OXPHOS inhibition partially restores suppressive capacity, confirming causality.
Yingying Chen, Hui Li
wiley   +1 more source

Arc-coloration et sommet-coloration orientées

, 2006
Dans ce mémoire, nous nous intéressons à trois notions de colorations de graphes orientés : la sommet-coloration orientée, l'arc-coloration orientée et l'étoile-coloration orientée acircuitique. La notion de sommet-coloration orientée a été introduite en
SOPENA, Eric, PINLOU, Alexandre
core  

In-Depth Exploration of the Coloration Mechanism of <i>Iris dichotoma</i> Pall. via Transcriptomic and Metabolomic Analyses. [PDF]

Plants (Basel)
Yu Y, Qiang X, Huang F, Huang X, Liu L.
europepmc   +1 more source

The C‐terminal domain of yeast Arginyltransferase1 is essential for its catalytic activity

FEBS Open Bio, EarlyView.
Arginyltransferase 1 (Ate1), a eukaryotic enzyme, catalyses arginylation, transferring arginine from tRNA‐Arg to the amino terminus of the target protein. Overexpression of Ate1 in yeast is lethal and is dependent on arginylation. This study elucidates how mutations in the cofactor‐binding and active site of Ate1 and truncation of its structural ...
Vikas Kumar Yadav, Shweta Shweta, Manisha Malhotra, Vikash Maurya, Akhilesh Kumar   +4 more
wiley   +1 more source

Metabolome and transcriptome integration reveals insights into petals coloration mechanism of three species in Sect. Chrysantha chang. [PDF]

PeerJ
Yan Y, Wang Y, Wen Y, Huang Y, Zhang M, Huang J, Li X, Wang C, Xu D.   +8 more
europepmc   +1 more source