Results 41 to 50 of about 178,057 (307)

Fabrication of a Malaria-Ab ELISA Bioassay Platform with Utilization of Syringe-Based and 3D Printed Assay Automation

Micromachines, 2018
We report on the fabrication of a syringe-based platform for automation of a colorimetric malaria-Ab assay. We assembled this platform from inexpensive disposable plastic syringes, plastic tubing, easily-obtainable servomotors, and an Arduino ...
Christopher Lim, Yangchung Lee, Lawrence Kulinsky   +2 more
doaj   +1 more source

In-Country Method Validation of a Paper-Based, Smartphone-Assisted Iron Sensor for Corn Flour Fortification Programs

Foods, 2022
Food fortification in low-income settings is limited due to the lack of simple quality control sensing tools. In this study, we field validated a paper-based, smartphone-assisted colorimetric assay (Nu3Px) for the determination of iron in fortified ...
Anna W. Waller, Marcela Gaytán-Martínez, Juan E. Andrade Laborde   +2 more
doaj   +1 more source

Enzyme Colorimetric Assay Using Unmodified Silver Nanoparticles

, 2016
Colorimetric assay based on the unique surface plasmon resonance properties of metallic nanoparticles has received considerable attention in bioassay due to its simplicity, high sensitivity, and low cost.
Erkang Wang (81595), Chaogui Chen (1952626), Bingyan Han (2365933), Hui Wei (126971)   +3 more
core   +1 more source

Dual PI3K/AKT and CDK4/6 inhibition reveals selective sensitivity in an SHH medulloblastoma stem cell model

Molecular Oncology, EarlyView.
Targeted therapy was evaluated in SHH medulloblastoma using neuroepithelial stem cell (NES) and tumor‐derived NES‐like (tNES) models in 2D monolayers and 3D spheroids. PI3K, AKT, and CDK4/6 inhibitors had minimal effects in NES but markedly reduced viability and growth and induced apoptosis in tNES cells, revealing distinct therapeutic vulnerabilities.
Monika Lukoseviciute, Madeleine Birgersson, Paolo Ceriani, Margareta Wilhelm, Ourania N Kostopoulou   +4 more
wiley   +1 more source

Colorimetric measurement of triglycerides cannot provide an accurate measure of stored fat content in Drosophila. [PDF]

PLoS ONE, 2010
Drosophila melanogaster has recently emerged as a useful model system in which to study the genetic basis of regulation of fat storage. One of the most frequently used methods for evaluating the levels of stored fat (triglycerides) in flies is a coupled ...
Bader Al-Anzi, Kai Zinn
doaj   +1 more source

Optical beam guidance in monolithic polymer chips for miniaturized colorimetric assays [PDF]

, 2005
For the first time, we present a simple and robust optical concept to enable precise and sensitive read-out of colorimetric assays in flat lab-on-a-chip devices.
J. Steigert, Riegger, Lutz, Urban, Gerald A., I. Moser, Moser, Isabella, Urban, G., Ducrée, Jens, G. Urban, Geipel, Andreas, Grumann, M., Moser, I., Steigert, J., Kohn, C., M. Grumann, J. Ducree, Grumann, Markus, L. Riegger, C. Kohn, A. Geipel, Zengerle, Roland, Riegger, L., Geipel, A., R. Zengerle, Steigert, Jürgen   +23 more
core   +1 more source

Loss of proton‐sensing TDAG8 increases tumor progression in mouse models of colon cancer

Molecular Oncology, EarlyView.
Loss of the pH‐sensing receptor TDAG8 accelerates colorectal cancer progression in mice. Animals lacking TDAG8 expression had increased tumor growth, DNA damage, and recruitment of tumor‐associated immune cells, including macrophages, neutrophils, and monocytes.
Ermanno Malagola, Yasmine Illi, Anna Bircher, Marijn Wilmink, Rachele F. Malagutti, Solenn Ottié, Cordelia Schuler, Pedro A. Ruiz, Cheryl de Vallière, Klaus Seuwen, Gerhard Rogler, Martin Hausmann   +11 more
wiley   +1 more source

Comparison of colorimetric assay with immunofluorescence-based focus-forming assay.

, 2012
(A) The number of foci in each well determined by colorimetric focus-forming assay with image analysis strongly correlated with that determined by immunofluorescence-based focus-forming assay and manual counting.
Wonseok Kang (139862), Eui-Cheol Shin (104030)   +1 more
core   +1 more source

Mycobacterial cell division arrest and smooth‐to‐rough envelope transition using CRISPRi‐mediated genetic repression systems

FEBS Open Bio, EarlyView.
CRISPRI‐mediated gene silencing and phenotypic exploration in nontuberculous mycobacteria. In this Research Protocol, we describe approaches to control, monitor, and quantitatively assess CRISPRI‐mediated gene silencing in M. smegmatis and M. abscessus model organisms.
Vanessa Point, Wafaa Achache, Janïs Laudouze, Eliana Sepulveda Ramos, Mickaël Maziero, Céline Crauste, Stéphane Canaan, Pierre Santucci   +7 more
wiley   +1 more source

From a Chemotherapeutic Drug to a High-Performance Nanocatalyst: A Fast Colorimetric Test for Cisplatin Detection at ppb Level

Biosensors, 2022
A rapid point-of-care method for the colorimetric detection of cisplatin was developed, exploiting the efficient conversion of the chemotherapeutic drug into a high-performance nanocatalyst with peroxidase enzyme mimics.
Valentina Mastronardi, Mauro Moglianetti, Edoardo Ragusa, Rodolfo Zunino, Pier Paolo Pompa   +4 more
doaj   +1 more source