Results 221 to 230 of about 120,673 (267)

Polymer Micropillar Arrays for Colorimetric DNA Detection

Analytical Chemistry, 2020
We describe the use of periodic micropillar arrays, produced from cyclic olefin copolymer using high-fidelity microfabrication, as templates for colorimetric DNA detection. The assay involves PCR-amplified gene markers for E. coli O157:H7 (rfbO157, eae, vt1, and vt2) incorporating a detectable digoxigenin label, which is revealed through an ...
Geissler, Matthias, Malic, Lidija, Morton, Keith J., Clime, Liviu, Daoud, Jamal, Hernández-Castro, Javier A., Corneau, Nathalie, Blais, Burton W., Veres, Teodor   +8 more
openaire   +2 more sources

Colorimetric Urinalysis for On-Site Detection of Metabolic Biomarkers

ACS Applied Materials & Interfaces, 2020
Over the past few decades, colorimetric assays have been developed for cost-effective and rapid on-site urinalysis. Most of these assays were employed for detection of biomarkers such as glucose, uric acid, ions, and albumin that are abundant in urine at micromolar to millimolar levels.
Sanjida Yeasmin, Gopal Ammanath, Yusuf Ali, Bernhard O. Boehm, Umit Hakan Yildiz, Alagappan Palaniappan, Bo Liedberg   +6 more
openaire   +3 more sources

Colorimetric-detected DNA sequencing

Analytical Biochemistry, 1987
A sensitive, colorimetric method for visualizing the band pattern of DNA sequencing reaction is described. The enzymatic incorporation of radioactive nucleotides commonly used for the band detection is replaced by biotin conjugated to the 5'-terminus of a synthetic oligonucleotide by chemical synthesis.
openaire   +3 more sources

Detection of ricin by colorimetric and chemiluminescence ELISA

Toxicon, 1994
A highly sensitive and specific ELISA was developed to detect ricin in biological fluids. The assay utilizes an affinity-purified goat polyclonal antibody to adsorb ricin from solution. The same antibody (biotinylated) is then used to form a sandwich, and avidin-linked alkaline phosphatase allows color development and measurement of optical density at ...
M A, Poli, V R, Rivera, J F, Hewetson, G A, Merrill   +3 more
openaire   +2 more sources

Point-of-care colorimetric detection with a smartphone

Lab on a Chip, 2012
Paper-based immunoassays are becoming powerful and low-cost diagnostic tools, especially in resource-limited settings. Inexpensive methods for quantifying these assays have been shown using desktop scanners, which lack portability, and cameras, which suffer from the ever changing ambient light conditions.
Li, Shen, Joshua A, Hagen, Ian, Papautsky   +2 more
openaire   +2 more sources

Colorimetric Detection of Cyanide with a Chromogenic Oxazine

Organic Letters, 2005
[reaction: see text] We have designed a chromogenic oxazine for the colorimetric detection of cyanide. Indeed, the [1,3]oxazine ring of our compound opens to form a 4-nitrophenylazophenolate chromophore in response to cyanide. This quantitative chromogenic transformation permits the detection of micromolar concentrations of cyanide in water ...
Massimiliano, Tomasulo, Françisco M, Raymo   +1 more
openaire   +2 more sources

A simple colorimetric detection of DNA methylation

The Analyst, 2012
In this work, we describe a simple colorimetric method to detect DNA methylation. Adenomatous polyposis coli (APC) with a small CpG region containing methylated cytosine (methylated APC) was synthesized and tested. Methylated APC was first captured and enriched by anti-5-methylcytosine monoclonal antibody conjugated magnetic microspheres (MMPs). Then a
Chenchen, Ge, Zhiyuan, Fang, Junhua, Chen, Jie, Liu, Xuewen, Lu, Lingwen, Zeng   +5 more
openaire   +2 more sources

Colorimetric Cotton Swab for Viral Protease Detection

Analytical Chemistry, 2022
Reporting the activity of a specific viral protease remains an acute need for rapid point-of-care detection strategies that can distinguish active infection from a resolved infection. In this work, we present a simple colorimetric approach for reporting the activity of a specific viral protease through direct color conversion on a cotton swab, which ...
Ranit Dutta, Sparsh Makhaik, Peiyao Zhao, Kristalle G. Cruz, Kwang-Won Park, Hongxu Liu, Trisha L. Andrew, Jeanne A. Hardy, S. Thayumanavan   +8 more
openaire   +2 more sources

The Colorimetric Detection and Quantitation of Total Protein

Current Protocols in Cell Biology, 2001
AbstractProtein quantification is an important step for handling protein samples for isolation and characterization; it is a prerequisite step before submitting proteins for chromatographic, electrophoretic, or immunochemical analysis and separation. Colorimetric methods are fast, simple, and not laborious.
openaire   +4 more sources

Colorimetric detection of ssDNA in a solution

Current Applied Physics, 2009
Abstract A solution containing ssDNA was detected by using colloidal gold nanoparticles upon a color change of solution. First of all, we prepared colloidal gold nanoparticles with a size of less than 20 nm by means of citrate reduction of HAuCl 4 . Since colloidal gold nanoparticles modified with citrate anions have a negative charge, they are very ...
Sangchul Rho, Sung Joong Kim, Sang Cheon Lee, Jeong Ho Chang, Hee-Gyoo Kang, Jinsub Choi   +5 more
openaire   +1 more source