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Colorimetric Detection of Acetyl Xylan Esterase Activities
2017Colorimetric detection of reaction products is typically preferred for initial surveys of acetyl xylan esterase (AcXE) activity. This chapter will describe common colorimetric methods, and variations thereof, for measuring AcXE activities on commercial, synthesized, and natural substrates.
Mai-Gisondi, Galina, Master, Emma R.
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Fluorescent and colorimetric sensors for environmental mercury detection
The Analyst, 2015The development of fluorescent and colorimetric sensing strategies for environmental mercury is described.
Guiqiu, Chen +3 more
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Novel plastic biochips for colorimetric detection of biomolecules
Analytical and Bioanalytical Chemistry, 2012We report a novel plastic biochip for the sensitive colorimetric detection of analytes of interest. This type of biochip is designed to perform bioassays in a sandwich format, i.e., employing the immobilized probe molecules to capture target and then utilizing gold nanoparticle (AuNP)-labeled reporters to screen the biorecognition events.
Jing, Wen +4 more
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A colorimetric and fluorescent probe for detecting intracellular biothiols
Biosensors and Bioelectronics, 2016A new rapid and highly sensitive coumarin-based probe (probe 1) has been designed and synthesized for detecting intracellular thiols. Probe 1 was prepared by a 4-step procedure as a latent fluorescence probe to achieve high sensitivity and fluorescence turn-on response toward cysteine and homocysteine over GSH and other various natural amino acids ...
Chunyang, Chen +3 more
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Detection of Carbohydrates by Colorimetric Methods
2021Classic colorimetric methods are still often used to detect the presence of carbohydrates. Several of these methods can distinguish between carbohydrate types and are also used for quantitative determinations. This chapter provides 18 protocols of different frequently used colorimetric methods, including their chemical reaction schemes.
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A fluorescent and colorimetric probe specific for palladium detection
Chem. Commun., 2010A selective Pd probe is described, which shows fluorometric and colorimetric responses towards both Pd(2+) ions and Pd(0)-complexes with excellent sensitivity and potential application. The fluorescence quenching effect of Pd(2+) is inhibited by filling up d-orbitals after the reduction from Pd(2+) (open-shell) to Pd(0) (closed-shell).
Honglin, Li +6 more
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A colorimetric sensor for the selective detection of fluoride ions
Luminescence, 2016AbstractA colorimetric receptor L was prepared. Receptor L can selectively sense F− based on distinct color changes among a series of ions. It can selectively sense F− through an intramolecular hydrogen bond interaction. A Job plot indicated a 1:1 complexation stoichiometry between receptor L and F−.
Chin-Feng, Wan +2 more
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A sensitive colorimetric detection of virus DNA and oncogene
Biochemical and Biophysical Research Communications, 1987Advantage of cloning probe DNA fragment in phage M13 DNA was taken to provide a larger single stranded DNA as a hybridization probe. High level of direct enzyme labels was introduced via the M13 DNA moiety as well as probe DNA. A highly sensitive colorimetric detection of virus DNA and oncogene was developed.
H, Inoue +3 more
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Colorimetric Cu2+ detection with a ligation DNAzyme and nanoparticles
Chemical Communications, 2007Using a Cu(2+)-dependent DNA ligation DNAzyme, a colorimetric sensor for Cu2+ has been developed based on directed assembly of DNA-functionalized gold nanoparticles by the ligation product, and such ligation DNAzyme-based sensors are intrinsically more sensitive than cleavage DNAzyme systems due to the lack of background.
Juewen, Liu, Yi, Lu
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Colorimetric Biosensors for Bacterial Detection
2016For thousands of years, humans have suffered from food poisoning as a result of microbial contamination. Pathogenic microbes can cause numerous illnesses, from minor skin infections to life-threatening diseases. Conventional detection techniques are often time-consuming, requiring trained personnel, sophisticated instruments, and long processing times,
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