Results 121 to 130 of about 451,802 (301)
FEBS Open Bio, EarlyView.This protocol paper outlines methods to establish the success of a time‐resolved serial crystallographic experiment, by means of statistical analysis of timepoint data in reciprocal space and models in real space. We show how to amplify the signal from excited states to visualise structural changes in successful experiments.
Jake Hill +4 more
wiley +1 more source
Heuristic branch-and-price for building long term trainee schedules. [PDF]
Branch-and-price is an increasingly important technique for solving large integer programming models. Staff scheduling has been a particularly fruitful area since these problems typically exhibit a decomposable structure.
Belien, Jeroen, Demeulemeester, Erik
core
A Simple Perspective on Simultaneous Column and Row Generation [PDF]
To solve a linear programming problem with many variables and constraints, a simultaneous column and row generation algorithm can be used. Such an algorithm iteratively solves a restricted version of a linear programming problem including only a subset ...
Spliet, Remy; id_orcid
core +1 more source
Screening and epitope characterization of Nidogen‐2‐specific nanobodies
FEBS Open Bio, EarlyView.Camel immunization and phage display were employed to generate high‐affinity VHH nanobodies against Nidogen‐2. After library construction, biopanning, ELISA screening, sequencing, and recombinant expression, selected nanobodies were purified and characterized, leading to the preliminary exploration of a nanobody‐based sandwich ELISA for specific ...
Jianchuan Wen +9 more
wiley +1 more source
A column generation approach to solve the crew re-scheduling problem
When tracks are out of service for maintenance during a certainperiod, trains cannot be operated on those tracks. This leads to amodified timetable, and results in infeasible rolling stock andcrew schedules. Therefore, these schedules need to be repaired.
Huisman, D.
core
FEBS Open Bio, EarlyView.Cytosolically synthesized chloroplast preproteins are translocated across the outer and inner envelope membranes through translocons called TOC and TIC, respectively. In green algae and plants, the TIC core is composed of essential membrane proteins, Tic12, Tic20, and Tic214.
Mengyi Li, Xueyang Zhao, Masato Nakai
wiley +1 more source
Rapid screening of staphylokinase protein variants using an unpurified cell‐free expression system
FEBS Open Bio, EarlyView.An unpurified cell‐free protein synthesis (CFPS) platform enables rapid functional screening of staphylokinase variants. Direct plasminogen‐activation assays performed in microplate format provide real‐time activity readouts, allowing rapid identification and ranking of variants with improved or reduced fibrinolytic activity without protein ...
Maria Tomková +3 more
wiley +1 more source
PENYELESAIAN MASALAH CUTTING STOCK DENGAN PENGELASAN MENGGUNAKAN TEKNIK COLUMN GENERATION [PDF]
, 2020 ABSTRAK Masalah cutting stock dengan pengelasan adalah permasalahan pemotongan dan pengelasan sejumlah bahan baku menjadi pola-pola tertentu untuk memenuhi permintaan dengan bahan baku sesedikit mungkin. Pada penelitian ini, masalah cutting stock dengan
Kamal Isham, -
core
Evolutionarily divergent DUF4465 domains have a common vitamin B12‐binding function
FEBS Open Bio, EarlyView.We show that DUF4465 family proteins, widespread across bacteria from gut microbiomes, hydrothermal vents, and soil, share a common vitamin B12‐binding function. These augmented β‐jellyroll proteins bind vitamin B12 via extended loops. Our findings establish sequence‐diverse DUF4465 proteins as a widespread class of B12‐binding proteins, highlighting ...
Charlea Clarke +4 more
wiley +1 more source
Systemic dysregulation of apolipoproteins in amyotrophic lateral sclerosis serum
FEBS Open Bio, EarlyView.Amyotrophic lateral sclerosis (ALS) is a fatal disease that damages motor neurons. This study found that people with ALS show significant changes in blood fats and the proteins that carry them. Several apolipoproteins were higher, lipid balances were altered, and normal protein–lipid relationships were disrupted.
Finula I. Isik +6 more
wiley +1 more source