Results 221 to 230 of about 864,970 (299)

Integration-coupled activation of promoterless combinatorial pathway libraries in Clostridium avoids burden during DNA assembly

Mordaka PM, Williamson J, Heap JT.
europepmc   +1 more source

Combinatorial optimization with Kerr solitons. [PDF]

Sci Adv
Jin Y, Chauhan N, Zang J, Edwards B, Chaudhari P, Aflatouni F, Papp SB.   +6 more
europepmc   +1 more source

Polarization‐resolved femtosecond Vis/IR spectroscopy tailored for resolving weak signals in biological samples using minimal sample volume

FEBS Open Bio, EarlyView.
Unique biological samples, such as site‐specific mutant proteins, are available only in limited quantities. Here, we present a polarization‐resolved transient infrared spectroscopy setup with referencing to improve signal‐to‐noise tailored towards tracing small signals. We provide an overview of characterizing the excitation conditions for polarization‐
Clark Zahn, Karsten Heyne
wiley   +1 more source

Analysing the significance of small conformational changes and low occupancy states in serial crystallographic data

FEBS Open Bio, EarlyView.
This protocol paper outlines methods to establish the success of a time‐resolved serial crystallographic experiment, by means of statistical analysis of timepoint data in reciprocal space and models in real space. We show how to amplify the signal from excited states to visualise structural changes in successful experiments.
Jake Hill, Yelyzaveta Pulnova, Elke De Zitter, Helen M. Ginn, Briony A. Yorke   +4 more
wiley   +1 more source

Energy-guided combinatorial co-optimization of antibody affinity and stability

Tennenhouse A, Mechaly A, Pham T, Oldham RJ, Henry J, Zhao DX, Moon TM, Elliott IG, Porat O, Kim J, Sirkis YF, Gaiduk S, Talpir I, Bird J, Domalewski C, Chu P, Dippel AB, Croasdale-Wood R, Christ D, Kaplan G, Cragg MS, Fleishman SJ.   +21 more
europepmc   +1 more source

Pharmacogenomics for stratified antidepressant treatment in major depressive disorder: evidence, limits and a roadmap for clinical use. [PDF]

Pharmacogenomics
Baune BT.
europepmc   +1 more source

Optimizing photoactivation of PA‐mCherry for optical pooled CRISPR screens

FEBS Open Bio, EarlyView.
Photoactivatable PA‐mCherry finds widespread use to optically tag individual cells. However, confocal 405 nm UV laser‐scanning (normal scan) is much less efficient than widefield UV illumination, limiting the use of PA‐mCherry on confocal instruments. We remedy this limitation by reporting that rapid and repeated confocal scanning with a low‐intensity,
Sravasti Mukherjee, Giulia Zanetti, Bram van den Broek, Kees Jalink   +3 more
wiley   +1 more source

Systematic design of auxotrophic strains and media conditions to probe metabolic functions in E. coli. [PDF]

PLoS Comput Biol
Mohammadbeygi R, Suthers PF, Chung FY, Pfleger BF, Maranas CD.   +4 more
europepmc   +1 more source

Refinement of amino‐acid conformation vs. difference density maps in time‐resolved serial femtosecond crystallography data analysis

FEBS Open Bio, EarlyView.
The dFoCC pipeline starts with observed DED and resting‐state coordinates, which are then used to generate a library of triggered states. Correlation analysis of the calculated DED features of each candidate vs observed DED permits quantitative evaluation of candidate structural quality.
Meng Iao Fong, Yuhei Hosokawa, Lars Oliver Essen, Manuel Maestre‐Reyna   +3 more
wiley   +1 more source

Rational engineering of combinatorial bacterial therapies for cancer. [PDF]

Genome Biol
Steppe P, O'Connor K, Hasty J.
europepmc   +1 more source