Results 61 to 70 of about 73,652 (267)

Evolution‐guided yeast complementation reveals functional differences in human PSPH variants

FEBS Open Bio, EarlyView.
Ancient genomes can help guide which human genetic variants are tested experimentally. This study applies that idea to PSPH, a gene involved in serine biosynthesis, and uses high‐throughput yeast complementation to compare variant function. The findings reveal measurable differences among selected alleles and illustrate the value of evolution‐guided ...
Mauricio Campa‐Álvarez, Diana Ascencio, Miguel Vallebueno‐Estrada, Eduardo González‐Orozco, Christian Eduardo Martínez‐Guerrero, Rafael Montiel, Alexander DeLuna   +6 more
wiley   +1 more source

Structural studies and functional engineering of NanX: an anhydro‐sialic acid transporter from Escherichia coli

FEBS Open Bio, EarlyView.
Biophysical characterisation shows that NanX, a membrane transport protein from the major facilitator superfamily (MFS), forms both monomers and dimers after purification. AlphaFold modelling and substrate docking provide information on residues likely involved in substrate recognition for NanX and another MFS member, NanT.
Michael C. Newton‐Vesty, Kelsi R. Hall, Zachary D. Tillett, Liam S. Turk, James S. Davies, Michael J. Currie, Hamish G. Brown, Sepideh Valimehr, Peter D. Mace, Andrew E. Whitten, Santosh Panjikar, Eric Hanssen, Rachel A. North, Renwick C.J. Dobson   +13 more
wiley   +1 more source

In silico and in vitro exploration of a tyrosinase for biocatalytic production of catechols

FEBS Open Bio, EarlyView.
Tyrosinase from Ralstonia pseudosolanacearum is a promising biocatalyst for producing valuable catechols from monophenol substrates. This tyrosinase is uniquely suited to this due to its high monophenolase : diphenolase ratio. We combined in silico docking and in vivo kinetic characterisation of this tyrosinase with 11 industrially relevant monophenols,
James Britton, Fang Zhao, Reeta Davis, Meg Walsh, Kevin E O'Connor, David O'Connell, Tanja Narancic   +6 more
wiley   +1 more source

Selection of high‐quality and viable blastocysts based on timing of morula compaction and blastocyst formation

Reproductive Medicine and Biology, 2020
Purpose The time‐lapse system is a device that allows continuous monitoring without removing embryos from the incubator. Using a time‐lapse system, we retrospectively investigated cleavage speed time points as potential indicators for selecting high ...
Yoshihisa Harada, Tomoyo Maeda, Emi Fukunaga, Reiko Shiba, Shinichiro Okano, Masayuki Kinutani, Toshitaka Horiuchi   +6 more
doaj   +1 more source

Adenosine triphosphate as a modulator of protein interactions and stability

FEBS Open Bio, EarlyView.
ATP is best known as the cell's energy currency, but it also shapes how proteins fold, interact, aggregate and form biomolecular condensates. This review explains the emerging physical principles behind these effects, including weak binding to charged protein regions, magnesium‐dependent behaviour and concentration‐dependent control of protein ...
Shuyuan Tan, Robin Curtis
wiley   +1 more source

Equipamento para coleta de amostras indeformadas de solo para estudos em condições controladas Undisturbed soil sampling equipment for research in controlled conditions

Revista Brasileira de Ciência do Solo, 2009
A avaliação da fertilidade do solo pode ser feita por vários métodos, entre eles os testes com plantas em campo ou em vasos. Estudos em casa de vegetação com solos sob sistema plantio direto são dificultados pela obtenção de amostras indeformadas.
Leandro Bortolon, Clesio Gianello, Osmar Conte, Elisandra Solange Oliveira, Renato Levien   +4 more
doaj   +1 more source

Purification and preparation of Marchantia polymorpha Auxin Response Factor 2 for phase separation studies

FEBS Open Bio, EarlyView.
We describe detailed protocols for the purification and preparation of Marchantia polymorpha Auxin Response Factor 2 (MpARF2). This protein is fused to an MBP solubility tag and an mNG fluorescent tag and is purified from Escherichia coli. The presented procedures make it possible to study MpARF2 assemblies, which could arise from phase separation ...
Bas Janssen, Robin Romein, Willy A. M. van den Berg, Simon Lindhoud, Jan Willem Borst, Carlo P. M. van Mierlo   +5 more
wiley   +1 more source

Heterotropic regulation and negative homotropic cooperativity

FEBS Open Bio, EarlyView.
We identified a structural module common to some proteins that couple negative cooperativity with heterotropic regulation, two features that rarely coexist. These proteins are ring‐like and present an ordered asymmetry whereby noncontacting subunits are symmetric, and their tertiary structure differs from that of contacting subunits.
Veronica Morea, Francesco Angelucci, Federica Arnesano, Gianmarco Pascarella, Patrizio Di Micco, Andrea Bellelli   +5 more
wiley   +1 more source

Statistical analysis of technical specifications of self-propelled reversible plate compactors with different types of engines

Вестник СибАДИ
Introduction. Reversible plate compactors are soil compaction machines with a flat operating device, having two or more unbalanced shafts and ability to reverse the direction and movement speed.
M. A. Afanasev, I. S. Tyuremnov
doaj   +1 more source

Ordinal compactness

Filomat, 2020
We introduce a new covering property, defined in terms of order types of sequences of open sets, rather than in terms of cardinalities. The most general form depends on two ordinal parameters. Ordinal compactness turns out to be a much more varied notion than cardinal compactness.
openaire   +5 more sources