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Advanced Functional Materials, EarlyView.This study introduces VIVID (Vesicle In Vivo Identification using DNA), a qPCR‐based platform that tracks PCR‐amplifiable DNA tags loaded in the EVs for accurate and quantifiable EV biodistribution in vivo. ABSTRACT Extracellular vesicles (EVs) represent promising carriers for nucleic acid therapeutics, offering advantages over synthetic nanoparticles ...
Oscar Boyadjian +5 more
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Bio‐Inspired Molecular Events in Poly(Ionic Liquids)
Advanced Functional Materials, EarlyView.Originating from dipolar and polar inter‐ and intra‐chain interactions of the building blocks, the topologies and morphologies of poly(ionic liquids) (PIL) govern their nano‐ and micro‐processibility. Modulating the interactions of cation‐anion pairs with aliphatic dipolar components enables the tunability of properties, facilitated by “bottom‐up ...
Jiahui Liu, Marek W. Urban
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TESTOSTERONE ASSAYS BY COMPETITIVE PROTEIN BINDING
Acta Endocrinologica, 1970ABSTRACT A review of competitive protein binding methods for the determination of testosterone in plasma is given. The different steps discussed are: Choice of the binding protein, dilution of the protein solution, separation of the free from the bound fraction, purification of the extract and factors determining the blank.
A, Vermeulen, L, Verdonck
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Competitive protein binding assay of corticosterone
Journal of Steroid Biochemistry, 1972Abstract Details of a competitive protein binding assay of corticosterone are described. The preliminary TLC separation permits simultaneous estimation of corticosterone, aldosterone and progesterone. “Unstressed” rat plasma corticosterone values were obtained between 0.3 and 9 μg/ 100 ml.
A, Spät, S, Józan
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Competitive Protein Binding Assay for Piritrexim
Journal of Pharmaceutical Sciences, 1989A competitive protein binding assay for piritrexim (PTX, 1) that makes use of a commercially available radioassay kit for methotrexate has been developed. After it is selectively extracted from plasma, PTX competes with [125l]methotrexate for binding to dihydrofolate reductase isolated from Lactobacillus casei. Free drug is separated from bound drug by
J L, Woolley, J L, Ringstad, C W, Sigel
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Competitive protein binding assay for 24,25-dihydroxycholecalciferol
Biochemical and Biophysical Research Communications, 1976Abstract A competitive protein binding assay which measures 24,25-dihydroxycholecalciferol in human serum has been developed using the binding protein from vitamin D-deficient rat kidney. As 25-hydroxycholecalciferol and 25,26-dihydroxycholecalciferol also interact with the binding protein, possible interference by these compounds in the assay has ...
C M, Taylor, S E, Hughes, P, de Silva
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Competitive binding assays for riboflavin and riboflavin-binding protein
Analytical Biochemistry, 1982Abstract A competitive binding procedure that can be used to determine either riboflavin or riboflavin-binding protein has been developed. Riboflavin-binding protein from chicken egg white binds tightly to DEAE-cellulose while free riboflavin does not.
S E, Lotter +3 more
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Competitive hapten-antibody binding: Solution of the binding equation
Journal of Theoretical Biology, 1965Abstract The competitive binding equation which describes the reactions between two univalent homogeneous cross-reacting haptens and antibody heterogeneous with respect to equilibrium constants has been solved. The joint probability density function of equilibrium constants can be computed directly from binding measurements; no assumption about the ...
J, Wong, F, Aladjem
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Analyzing competitive binding data
2004Abstract Competitive binding experiments measure the binding of a single concentration of labeled ligand in the presence of various concentrations of unlabeled ligand. Ideally, the concentration of unlabeled ligand varies over at least six orders of magnitude.
Harvey Motulsky, Arthur Christopoulos
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Homologous competitive binding curves
2004Abstract The most common way to determine receptor number ,, nd affinity is to perform a saturation binding experiment, where you vary the concentration of radioligand. An alternative is to keep the radioligand concentration constant, and compete for binding with the same ligand, but not radioactively labeled. Since the hot (radiolabeled)
Harvey Motulsky, Arthur Christopoulos
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