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Analyzing competitive binding data
2004Abstract Competitive binding experiments measure the binding of a single concentration of labeled ligand in the presence of various concentrations of unlabeled ligand. Ideally, the concentration of unlabeled ligand varies over at least six orders of magnitude.
Harvey Motulsky, Arthur Christopoulos
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Competitive protein-binding radioassay for retinoic acid
Analytical Biochemistry, 1980Rat testes cytosol treated by Blue Sepharose was employed in a simple and sensitive method for the determination of retinoic acid in the rat serum, liver, and intestine. The method permits the detection of as little as 3 ng of retinoic acid. The mean concentrations of retinoic acid in normal male rats were 33.5 ng/ml of serum, 624.9 ng/g wet wt of ...
Y, Shidoji, N, Hosoya
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Homologous competitive binding curves
2004Abstract The most common way to determine receptor number ,, nd affinity is to perform a saturation binding experiment, where you vary the concentration of radioligand. An alternative is to keep the radioligand concentration constant, and compete for binding with the same ligand, but not radioactively labeled.
Harvey Motulsky, Arthur Christopoulos
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Competitive Binding Among Oral Streptococci to Hydroxyapatite
Journal of Dental Research, 1977The relative affinity of various oral streptococci for hydroxyapatite (HA) differed widely. Streptococcus mutans affinity for dextran-coated HA was the highest of all the streptococci to any HA coating. S salivarius had the lowest affinity, and S sanguis affinity was higher than S miteor for saliva-coated HA.
W F, Liljemark, S V, Schauer
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A direct competitive binding radioimmunoassay for carcinoembryonic antigen
Journal of Immunological Methods, 1980We have incorporated commercially available CEA standard and antiserum into the triple isotope double antibody radioimmunoassay and we have evaluated this assay for the routine determination of CEA. The competitive protein binding (CPB) assay for CEA can be performed directly on serum or plasma without perchloric acid extraction.
H A, Fritsche +4 more
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Adrenocorticotropin Measurement by Competitive Binding Receptor Assay
The Journal of Clinical Endocrinology & Metabolism, 1972A competitive binding assay using the cortical receptor from normal adrenal glands has been developed for ACTH. In this assay porcine, human or rabbit ACTH has been shown to compete with labeled porcine ACTH for the cortical receptor from ovine, rabbit or rat adrenal glands.
A R, Wolfsen, H B, McIntyre, W D, Odell
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Zona Binding: Competitive Sperm-Binding Assay
2021De Yi Liu, Ying Zhong, Zi-Na Wen
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Potential misinterpretation of the competitive binding assays
Trends in Biochemical Sciences, 1989E, Presecan, H, Porumb, I, Lascu
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