Results 241 to 250 of about 2,770,096 (297)

Competitive hapten-antibody binding: Solution of the binding equation

Journal of Theoretical Biology, 1965
Abstract The competitive binding equation which describes the reactions between two univalent homogeneous cross-reacting haptens and antibody heterogeneous with respect to equilibrium constants has been solved. The joint probability density function of equilibrium constants can be computed directly from binding measurements; no assumption about the ...
J, Wong, F, Aladjem
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TESTOSTERONE ASSAYS BY COMPETITIVE PROTEIN BINDING

Acta Endocrinologica, 1970
ABSTRACT A review of competitive protein binding methods for the determination of testosterone in plasma is given. The different steps discussed are: Choice of the binding protein, dilution of the protein solution, separation of the free from the bound fraction, purification of the extract and factors determining the blank.
A, Vermeulen, L, Verdonck
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Competitive protein binding assay of corticosterone

Journal of Steroid Biochemistry, 1972
Abstract Details of a competitive protein binding assay of corticosterone are described. The preliminary TLC separation permits simultaneous estimation of corticosterone, aldosterone and progesterone. “Unstressed” rat plasma corticosterone values were obtained between 0.3 and 9 μg/ 100 ml.
A, Spät, S, Józan
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Competitive Binding Study Revealing the Influence of Fluorophore Labels on Biomolecular Interactions

Nano Letters, 2019
Dietz MS, Wehrheim SS, Harwardt M-LIE, Niemann H, Heilemann M. Competitive Binding Study Revealing the Influence of Fluorophore Labels on Biomolecular Interactions. NANO LETTERS.
Hartmut H Niemann, Mike Heilemann, Marina S Dietz   +2 more
exaly   +2 more sources

Effect of transport and competition on ligand binding

Biophysical Chemistry, 1999
We present a model to describe the physics of chemoreception in processes determined by competitive ligand binding. Our model describes the competition between various populations, such as ligands vs. blockers and receptors vs. decoys, in protein activation when diffusion is rate-determining.
C. A. CONDAT, DELSANTO, PIER PAOLO, E. RUFFINO, G. PEREGO   +3 more
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Cefmenoxime and Bilirubin: Competition for Albumin Binding

Pharmacology & Toxicology, 1991
Abstract:Certain drugs are known to compete with bilirubin for albumin binding; therefore, all drugs administered to neonates should be tested to determine the degree of competition. The effect of cefmenoxime on bilirubin‐albumin binding was determined by comparing the oxidation rate of free bilirubin in the presence and absence of drug.
D L, Onks, J F, Harris, A F, Robertson
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ASSAY OF ALDOSTERONE BY COMPETITIVE PROTEIN BINDING

Acta Endocrinologica, 1970
ABSTRACT We have applied a competitive protein binding technique to the assay of aldosterone, utilizing the aldosterone binding protein (ABP) in kidney as the specific receptor molecule. Intracellular ABP was obtained from the homogenization of adrenalectomized rat kidneys in 0.25 M sucrose.
R G, Robinson, D D, Fanestil
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Competitive Protein Binding Assay for Piritrexim

Journal of Pharmaceutical Sciences, 1989
A competitive protein binding assay for piritrexim (PTX, 1) that makes use of a commercially available radioassay kit for methotrexate has been developed. After it is selectively extracted from plasma, PTX competes with [125l]methotrexate for binding to dihydrofolate reductase isolated from Lactobacillus casei. Free drug is separated from bound drug by
J L, Woolley, J L, Ringstad, C W, Sigel
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Determination of calmodulin by competitive binding assay

Analytical Biochemistry, 1986
Calmodulin levels in tissue or cellular extracts can be determined by competition with 125I-calmodulin in a filtration-based direct binding assay. The method is rapid, uses readily available stable components, and possesses a selectivity and sensitivity comparable to that observed with immunoassay and phosphodiesterase activation. This assay provides a
H, LeVine, N, Sahyoun, P, Cuatrecasas
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Measurement of thyroxine by competitive protein binding

Clinica Chimica Acta, 1972
Abstract A simplified method is described for the measurement of thyroxine by competitive protein binding, using Sephadex columns for separation of bound from unbound thyroxine. Conditions for the various steps in the method were studied. The results compare well with PBI determinations done on the same subjects.
H, Seligson, D, Seligson
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