Results 111 to 120 of about 25,476 (265)

From tumor‐centric to ecosystem‐based hypotheses in brain tumor research and care

Molecular Oncology, EarlyView.
Primary brain tumors, whether in adults or children, present a major challenge because of their dramatic prognosis and the ongoing lack of efficient therapeutic approaches. In recent years, a shift has occurred from tumor‐centric concepts to a more holistic view of these tumors as dynamic ecosystems.
Julie Gavard, Flavie Bénard, Eva Billat, Margaux Le Guyon, Victoria Maltret, Laura Merlet, Chloé Astrid Walter, Tony Avril, Eric Chevet   +8 more
wiley   +1 more source

Temperature and Stretching Effects on Complementarity Determining Regions (CDRs) Conformation and Stability of Nimotuzumab F(ab)-Fragment

Atom Indonesia, 2015
Nimotuzumab is a humanized monoclonal antibody (mAb), a potential anticancer against epidermal growth factor receptor (EGFR)overexpressed by glioma, head and neck, lung, ovarium, and colon cancers.
T.S. Humani , M.A. Martoprawiro , A. Mutalib   +2 more
doaj  

Automated FRAP microscopy for high‐throughput analysis of protein dynamics in chromatin organization and transcription

FEBS Open Bio, EarlyView.
RoboMic is an automated confocal microscopy pipeline for high‐throughput functional imaging in living cells. Demonstrated with fluorescence recovery after photobleaching (FRAP), it integrates AI‐driven nuclear segmentation, ROI selection, bleaching, and analysis.
Selçuk Yavuz, Bart Geverts, Johan A. Slotman, Andrea Sacchetti, Stefan Prekovic, Martin E. van Royen, Adriaan B. Houtsmuller   +6 more
wiley   +1 more source

Role of complementarity-determining regions 1 and 3 in pathologic amyloid formation by human immunoglobulin κ1 light chains. [PDF]

Amyloid, 2023
Klimtchuk ES, Peterle D, Bullitt E, Connors LH, Engen JR, Gursky O.   +5 more
europepmc   +1 more source

Antibody complementarity-determining region design using AlphaFold2 and DDG predictor

The Journal of Supercomputing
AbstractThe constraints imposed by natural antibody affinity maturation often culminate in antibodies with suboptimal binding affinities, thereby limiting their therapeutic efficacy. As such, the augmentation of antibody binding affinity is pivotal for the advancement of efficacious antibody-based therapies.
Takafumi Ueki, Masahito Ohue
openaire   +1 more source

A combinatorial approach for the design of complementarity determining regions (CDR)-derived peptidomimetics with in vitro anti-tumoral activity [PDF]

, 2009
9 páginas, 6 figuras, 1 tabla -- PAGS nros. 34126-34134The great success of therapeutic monoclonal antibodies has fueled research toward mimicry of their binding sites and the development of new strategies for peptide-based mimetics production.
Barderas, Rodrigo, Altschuh, Danièle, Hollestelle, Martine J., Monasterio, Alberto, Desmet, Johan, Höppener, Jo W.M., Timmerman, Peter, Casal, J. Ignacio, Shochat, Susana Geifman, Meloen, Rob H.   +9 more
core   +1 more source

Transitions of CDR-L3 Loop Canonical Cluster Conformations on the Micro-to-Millisecond Timescale

Frontiers in Immunology, 2019
Sequence and structural diversity of antibodies are concentrated on six hypervariable loops, also known as the complementarity determining regions (CDRs).
Monica L. Fernández-Quintero, Barbara A. Math, Johannes R. Loeffler, Klaus R. Liedl   +3 more
doaj   +1 more source

Improving antibody affinity through in vitro mutagenesis in complementarity determining regions. [PDF]

J Biomed Res, 2022
Ye W, Liu X, He R, Gou L, Lu M, Yang G, Wen J, Wang X, Liu F, Ma S, Qian W, Jia S, Ding T, Sun L, Gao W.   +14 more
europepmc   +1 more source

A light‐triggered Time‐Resolved X‐ray Solution Scattering (TR‐XSS) workflow with application to protein conformational dynamics

FEBS Open Bio, EarlyView.
Time‐resolved X‐ray solution scattering captures how proteins change shape in real time under near‐native conditions. This article presents a practical workflow for light‐triggered TR‐XSS experiments, from data collection to structural refinement. Using a calcium‐transporting membrane protein as an example, the approach can be broadly applied to study ...
Fatemeh Sabzian‐Molaei, Fredrik Orädd, Konstantinos Magkakis, Magnus Andersson   +3 more
wiley   +1 more source

Extending Native Top-Down Electron Capture Dissociation to MDa Immunoglobulin Complexes Provides Useful Sequence Tags Covering Their Critical Variable Complementarity-Determining Regions. [PDF]

Anal Chem, 2021
Greisch JF, den Boer MA, Lai SH, Gallagher K, Bondt A, Commandeur J, Heck AJR.   +6 more
europepmc   +1 more source