Results 1 to 10 of about 2,891,675 (191)

Rescue of tomato spotted wilt virus entirely from complementary DNA clones. [PDF]

open access: yesProc Natl Acad Sci U S A, 2020
Significance Although reverse genetics (RG) systems were first developed for animal-infecting viruses with segmented negative-stranded/ambisense RNA genomes over 20 years ago, such a system for plant viruses with segmented negative-stranded/ambisense RNA
Feng M   +11 more
europepmc   +2 more sources

Validation of the nearest-neighbor model for Watson-Crick self-complementary DNA duplexes in molecular crowding condition. [PDF]

open access: yesNucleic Acids Res, 2019
Recent advancement in nucleic acid techniques inside cells demands the knowledge of the stability of nucleic acid structures in molecular crowding.
Ghosh S   +5 more
europepmc   +2 more sources

Novel codons in rat Pdx-1 complementary DNA [PDF]

open access: yesBMC Research Notes, 2018
Objectives Pancreatic and duodenal homeobox-1 (Pdx-1) is a homeodomain-containing transcription factor essential for pancreatic development, beta-cell differentiation and the maintenance of mature beta cell function.
Takayoshi Kiba
doaj   +2 more sources

Yeast Pif1 accelerates annealing of complementary DNA strands. [PDF]

open access: yesBiochemistry, 2014
Pif1 is a helicase involved in the maintenance of nuclear and mitochondrial genomes in eukaryotes. Here we report a new activity of Saccharomyces cerevisiae Pif1, annealing of complementary DNA strands. We identified preferred substrates for annealing as
Ramanagoudr-Bhojappa R   +3 more
europepmc   +2 more sources

HLA genotyping by next-generation sequencing of complementary DNA [PDF]

open access: yesBMC Genomics, 2017
Background Genotyping of the human leucocyte antigen (HLA) is indispensable for various medical treatments. However, unambiguous genotyping is technically challenging due to high polymorphism of the corresponding genomic region.
Hidenobu Segawa   +2 more
doaj   +2 more sources

Studies on the expression of the microtubule-associated protein, tau, during mouse brain development, with newly isolated complementary DNA probes. [PDF]

open access: bronzeJournal of Cell Biology, 1984
Tau protein is a collection of closely related polypeptides that associate with microtubules in vivo and stimulate their assembly in vitro. Using an affinity-purified antiserum against bovine brain tau protein, we found that the number and amount of tau ...
David G. Drubin   +2 more
openalex   +2 more sources

Rapid and Sensitive Detection of Severe Acute Respiratory Syndrome Coronavirus 2 in Label-Free Manner Using Micromechanical Sensors

open access: yesSensors, 2021
Coronavirus (COVID-19), caused by the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), has been identified as a deadly pandemic. The genomic analysis of SARS-CoV-2 is performed using a reverse transcription-polymerase chain reaction (RT-PCR)
Dalal A. Aloraini   +4 more
doaj   +1 more source

Synthesis and Evaluation of MGB Polyamide-Oligonucleotide Conjugates as Gene Expression Control Compounds

open access: yesJournal of Nucleic Acids, 2023
MGB polyamide-oligonucleotide conjugates ON 1-4 with linked MGB polyamides at the 2-exocyclic amino group of a guanine base using aminoalkyl linkers were synthesized and evaluated in terms of binding affinity for complementary DNA containing the MGB ...
Kazuo Kamaike   +13 more
doaj   +1 more source

A Brief Review of Graphene-Based Biosensors Developed for Rapid Detection of COVID-19 Biomarkers

open access: yesBiosensors, 2023
The prevalence of mutated species of COVID-19 antigens has provided a strong impetus for identifying a cost-effective, rapid and facile strategy for identifying the viral loads in public places.
Narendhar Chandrasekar   +7 more
doaj   +1 more source

Design strategy of antidote sequence for bivalent aptamer: Rapid neutralization of high‐anticoagulant thrombin‐binding bivalent DNA aptamer‐linked M08 with HD22

open access: yesResearch and Practice in Thrombosis and Haemostasis, 2021
Background Bivalent thrombin‐binding aptamers (TBAs) have great potential for the treatment of thrombosis because they exhibit high anticoagulant activity, and their complementary single‐stranded DNA (ssDNA) sequences work as an antidote.
Toru Yoshitomi   +3 more
doaj   +1 more source

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