Rescue of tomato spotted wilt virus entirely from complementary DNA clones. [PDF]
Significance Although reverse genetics (RG) systems were first developed for animal-infecting viruses with segmented negative-stranded/ambisense RNA genomes over 20 years ago, such a system for plant viruses with segmented negative-stranded/ambisense RNA
Feng M+11 more
europepmc +2 more sources
Validation of the nearest-neighbor model for Watson-Crick self-complementary DNA duplexes in molecular crowding condition. [PDF]
Recent advancement in nucleic acid techniques inside cells demands the knowledge of the stability of nucleic acid structures in molecular crowding.
Ghosh S+5 more
europepmc +2 more sources
Novel codons in rat Pdx-1 complementary DNA [PDF]
Objectives Pancreatic and duodenal homeobox-1 (Pdx-1) is a homeodomain-containing transcription factor essential for pancreatic development, beta-cell differentiation and the maintenance of mature beta cell function.
Takayoshi Kiba
doaj +2 more sources
Yeast Pif1 accelerates annealing of complementary DNA strands. [PDF]
Pif1 is a helicase involved in the maintenance of nuclear and mitochondrial genomes in eukaryotes. Here we report a new activity of Saccharomyces cerevisiae Pif1, annealing of complementary DNA strands. We identified preferred substrates for annealing as
Ramanagoudr-Bhojappa R+3 more
europepmc +2 more sources
HLA genotyping by next-generation sequencing of complementary DNA [PDF]
Background Genotyping of the human leucocyte antigen (HLA) is indispensable for various medical treatments. However, unambiguous genotyping is technically challenging due to high polymorphism of the corresponding genomic region.
Hidenobu Segawa+2 more
doaj +2 more sources
Bioelectronic DNA detection of human papillomaviruses using eSensor™: a model system for detection of multiple pathogens [PDF]
Background We used human papillomaviruses (HPV) as a model system to evaluate the utility of a nucleic acid, hybridization-based bioelectronic DNA detection platform (eSensor™) in identifying multiple pathogens.
Miller Donna L+7 more
doaj +4 more sources
Hyperchaotic color image encryption using eight-base DNA complementary rules and extended Zigzag transform. [PDF]
As an efficient technique to protect image content from unauthorized access, image encryption has been a hot spot in recent studies. Among various schemes of image encryption, those that utilize hyperchaotic systems and deoxyribonucleicacid (DNA ...
Bin Yang
doaj +2 more sources
Transcriptome analysis by strand-specific sequencing of complementary DNA [PDF]
High-throughput complementary DNA sequencing (RNA-Seq) is a powerful tool for whole-transcriptome analysis, supplying information about a transcript's expression level and structure.
D. Parkhomchuk+7 more
semanticscholar +5 more sources
Complementary recognition in condensed DNA: Accelerated DNA renaturation [PDF]
The functional consequences of DNA condensation are investigated. The recognition of complementary strands is profoundly modified by this critical phenomenon. (1) Condensation of denatured DNA greatly accelerates the kinetics of DNA renaturation. We propose a unifying explanation for the effects of several accelerating solvents studied here including ...
Jean‐Louis Sikorav, George M. Church
openalex +4 more sources
Studies on the expression of the microtubule-associated protein, tau, during mouse brain development, with newly isolated complementary DNA probes. [PDF]
Tau protein is a collection of closely related polypeptides that associate with microtubules in vivo and stimulate their assembly in vitro. Using an affinity-purified antiserum against bovine brain tau protein, we found that the number and amount of tau ...
David G. Drubin+2 more
openalex +2 more sources