Results 21 to 30 of about 806,056 (297)

The Enhanced Sensitivity of a Porous Silicon Microcavity Biosensor Based on an Angular Spectrum Using CdSe/ZnS Quantum Dots

Sensors, 2019
To improve the detection sensitivity of porous silicon microcavity biosensors, CdSe/ZnS quantum dots are used to label complementary DNA molecules for the refractive index amplification and angular spectrum method for detection.
Rui Zhou, Zhenhong Jia, Xiaoyi Lv, Xiaohui Huang   +3 more
doaj   +1 more source

Structural basis for CRISPR RNA-guided DNA recognition by Cascade [PDF]

, 2011
The CRISPR (clustered regularly interspaced short palindromic repeats) immune system in prokaryotes uses small guide RNAs to neutralize invading viruses and plasmids.
A Bolotin, A Noy, AJR Heck, AL Gnatt, Albert J R Heck, AM Maxam, Ambrosius P L Snijders, Arjan Barendregt, B Wiedenheft, Blake Wiedenheft, C Hale, C Pourcel, CR Hale, D Han, DH Haft, DY Lee, Edze R Westra, Egbert J Boekema, Esther van Duijn, FJ Mojica, FJ Mojica, FV Karginov, GT Oostergetel, H Deveau, H Deveau, J Carte, J Li, J van der Oost, JB Ma, JE Garneau, Jelle B Bultema, Jennifer A Doudna, John van der Oost, K Lorenzen, K Yu, Kaihong Zhou, KS Makarova, LA Marraffini, LA Marraffini, LA Marraffini, M Thomas, M van Heel, M Zhou, Magnus Lundgren, Marieke R Beijer, Mark J Dickman, Matthijs M Jore, MJ Dickman, MR Singleton, N Tahallah, P Horvath, P Horvath, R Barrangou, R Jansen, RE Haurwitz, Reinhild Wurm, RH van den Heuvel, RK Lillestøl, Rolf Wagner, S Houwing, Sakharam P Waghmare, SC Raghavan, SJ Brouns, SP Waghmare, Stan J J Brouns, T Itoh, T Wilson-Sali, TA Baker, TH Tang, V Kunin, Y Agari, Ümit Pul   +71 more
core   +5 more sources

Mechanical separation of the complementary strands of DNA [PDF]

Proceedings of the National Academy of Sciences, 1997
We describe the mechanical separation of the two complementary strands of a single molecule of bacteriophage λ DNA. The 3′ and 5′ extremities on one end of the molecule are pulled progressively apart, and this leads to the opening of the double helix. The typical forces along the opening are in the range of 10–15 pN. The separation force signal is
B, Essevaz-Roulet, U, Bockelmann, F, Heslot   +2 more
openaire   +2 more sources

Fabrication and functionalization of PCB gold electrodes suitable for DNA-based electrochemical sensing [PDF]

, 2014
The request of high specificity and selectivity sensors suitable for mass production is a constant demand in medical research. For applications in point-of-care diagnostics and therapy, there is a high demand for low cost and rapid sensing platforms ...
Dhaenens, Kristof, Gielen, An, Henry, OYF, Lundeberg, J, O'Sullivan, CK, Salvo, Pietro, Sanchez, JLA, Solnestam, BW, Vanfleteren, Jan   +8 more
core   +1 more source

Inhibition of BRCA2 and Thymidylate Synthase Creates Multidrug Sensitive Tumor Cells via the Induction of Combined “Complementary Lethality”

Molecular Therapy: Nucleic Acids, 2013
A high mutation rate leading to tumor cell heterogeneity is a driver of malignancy in human cancers. Paradoxically, however, genomic instability can also render tumors vulnerable to therapeutic attack. Thus, targeting DNA repair may induce an intolerable
Mateusz Rytelewski, Peter J Ferguson, Saman Maleki Vareki, Rene Figueredo, Mark Vincent, James Koropatnick   +5 more
doaj   +1 more source

Complementary recognition in condensed DNA: Accelerated DNA renaturation [PDF]

Journal of Molecular Biology, 1991
The functional consequences of DNA condensation are investigated. The recognition of complementary strands is profoundly modified by this critical phenomenon. (1) Condensation of denatured DNA greatly accelerates the kinetics of DNA renaturation. We propose a unifying explanation for the effects of several accelerating solvents studied here including ...
J L, Sikorav, G M, Church
openaire   +2 more sources

Parallel DNA polymerase chain reaction: Synthesis of two different PCR products from a DNA template [v1; ref status: indexed, http://f1000r.es/4sm]

F1000Research, 2014
Conventionally, in a polymerase chain reaction (PCR), oligonucleotide primers bind to the template DNA in an antiparallel complementary way and the template DNA is amplified as it is.
Vikash Bhardwaj, Kulbhushan Sharma
doaj   +1 more source

Structural assessment of the full-length wild-type tumor suppressor protein p53 by mass spectrometry-guided computational modeling

Scientific Reports, 2023
The tetrameric tumor suppressor p53 represents a great challenge for 3D-structural analysis due to its high degree of intrinsic disorder (ca. 40%). We aim to shed light on the structural and functional roles of p53’s C-terminal region in full-length ...
Alessio Di Ianni, Christian Tüting, Marc Kipping, Christian H. Ihling, Janett Köppen, Claudio Iacobucci, Christian Arlt, Panagiotis L. Kastritis, Andrea Sinz   +8 more
doaj   +1 more source

Method of identifying inhibitors of glutathione S-transferase (GST) gene expression [PDF]

, 1999
Complementary DNA and genomic clones for three variants of GST-.pi. are disclosed. It is demonstrated that certain of these variants are overexpressed in gliomas, thereby indicating an involvement with that form of cancer.
Akande, Olanike, Ali-Osman, Francis, Antoun, Gamil, Buolamwini, John K., Keller, Charles W., Lo, Hui-Wen, Lopez-Berestein, Gabriel   +6 more
core   +1 more source

Efficiency of peptide nucleic acid-directed PCR clamping and its application in the investigation of natural diets of the Japanese eel leptocephali. [PDF]

PLoS ONE, 2011
Polymerase chain reaction (PCR)-clamping using blocking primer and DNA-analogs, such as peptide nucleotide acid (PNA), may be used to selectively amplify target DNA for molecular diet analysis.
Takeshi Terahara, Seinen Chow, Hiroaki Kurogi, Sun-Hee Lee, Katsumi Tsukamoto, Noritaka Mochioka, Hideki Tanaka, Haruko Takeyama   +7 more
doaj   +1 more source