Results 1 to 10 of about 2,650,464 (200)

A Novel Continuous-Flow PCR Microdevice Operated by a Single Heat Source [PDF]

Micromachines
This paper presents a constant-temperature, single-heat-source continuous-flow PCR (CF-PCR) microdevice that achieves stable thermal control for denaturation, annealing, and extension on a single platform. Key innovations include: (1) a metal-powder/PDMS
Weining Song, Di Wu, Yutong Xing, Wenming Wu   +3 more
doaj   +2 more sources

Unlocked nucleic acid (UNA) and UNA derivatives: Thermal denaturation studies [PDF]

Nucleic Acids Symposium Series, 2008
A study on the thermal stability of duplexes formed between unlocked nucleic acid (UNA) modified DNA and RNA oligonucleotides and complementary DNA and RNA is presented. The acyclic UNA monomers are shown to induce a decrease in duplex thermal stability.
Jesper Wengel
exaly   +4 more sources

Fast and non-toxic in situ hybridization without blocking of repetitive sequences. [PDF]

PLoS ONE, 2012
Formamide is the preferred solvent to lower the melting point and annealing temperature of nucleic acid strands in in situ hybridization (ISH). A key benefit of formamide is better preservation of morphology due to a lower incubation temperature. However,
Steen H Matthiesen, Charles M Hansen
doaj   +1 more source

Sequence-Specific Recognition of Double-Stranded DNA by Peptide Nucleic Acid Forming Double-Duplex Invasion Complex

Applied Sciences, 2022
Peptide nucleic acid (PNA) is an analog of natural nucleic acids, where the sugar-phosphate backbone of DNA is replaced by an electrostatically neutral N-(2-aminoethyl)glycine backbone.
Yuichiro Aiba, Masanari Shibata, Osami Shoji   +2 more
doaj   +1 more source

Ultrafast Microfluidic PCR Thermocycler for Nucleic Acid Amplification

Micromachines, 2023
The polymerase chain reaction (PCR) is essential in nucleic acid amplification tests and is widely used in many applications such as infectious disease detection, tumor screening, and food safety testing; however, most PCR devices have inefficient ...
Yi-Quan An, Shao-Lei Huang, Bang-Chao Xi, Xiang-Lian Gong, Jun-Hao Ji, You Hu, Yi-Jie Ding, Dong-Xu Zhang, Sheng-Xiang Ge, Jun Zhang, Ning-Shao Xia   +10 more
doaj   +1 more source

Regulatory effect of apoptosis on morphological changes in cell mass of porcine blastocyst through supplementation of rapamycin during in vitro culture

Journal of Advanced Veterinary and Animal Research, 2020
Objective: The study of an in vitro embryosis is crucial in genetics for breed improvement and reproduction in livestock, identifying the causes of infertility, and stem cell application.
Dae Seung Kim, Sang Hwan Kim, Jong Taek Yoon   +2 more
doaj   +1 more source

Investigation of the Characteristics of NLS-PNA: Influence of NLS Location on Invasion Efficiency

Applied Sciences, 2020
Peptide nucleic acid can recognise sequences in double-stranded DNA (dsDNA) through the formation of a double-duplex invasion complex. This double-duplex invasion is a promising method for the recognition of dsDNA in cellula because peptide nucleic acid (
Yuichiro Aiba, Gerardo Urbina, Masanari Shibata, Osami Shoji   +3 more
doaj   +1 more source

Individual Case Analysis of Postmortem Interval Time on Brain Tissue Preservation. [PDF]

PLoS ONE, 2016
At autopsy, the time that has elapsed since the time of death is routinely documented and noted as the postmortem interval (PMI). The PMI of human tissue samples is a parameter often reported in research studies and comparable PMI is preferred when ...
Jeffrey A Blair, Chunyu Wang, Damarys Hernandez, Sandra L Siedlak, Mark S Rodgers, Rojan K Achar, Lara M Fahmy, Sandy L Torres, Robert B Petersen, Xiongwei Zhu, Gemma Casadesus, Hyoung-Gon Lee   +11 more
doaj   +1 more source

Physiological and biochemical responses of Yarrowia lipolytica to dehydration induced by air-drying and freezing. [PDF]

PLoS ONE, 2014
Organisms that can withstand anhydrobiosis possess the unique ability to temporarily and reversibly suspend their metabolism for the periods when they live in a dehydrated state.
Caroline Pénicaud, Sophie Landaud, Frédéric Jamme, Pauline Talbot, Marielle Bouix, Sarrah Ghorbal, Fernanda Fonseca   +6 more
doaj   +1 more source

Single-cell analysis reveals microbial spore responses to microwave radiation

Journal of Innovative Optical Health Sciences, 2023
To determine the effects of microwave radiation at the molecular level as well as on the germination, growth and morphology of dry spores at the single-cell level.
Siyi Qiu, Haihua Fan, Lin He
doaj   +1 more source