Results 31 to 40 of about 2,650,464 (200)

Partial Denaturation of Double-Stranded DNA on Pristine Graphene under Physiological-like Conditions

Liquids, 2023
Interactions between DNA and graphene are paramount for a wide range of applications, such as biosensing and nanoelectronics; nonetheless, the molecular details of such interactions remain largely unexplored.
Fernando J. A. L. Cruz, José P. B. Mota
doaj   +1 more source

PCR performance of a thermostable heterodimeric archaeal DNA polymerase

Frontiers in Microbiology, 2014
DNA polymerases are versatile tools used in numerous important molecular biological core technologies like the ubiquitous polymerase chain reaction (PCR), cDNA cloning, genome sequencing and nucleic acid based diagnostics.
Tom eKillelea, Celine eRalec, Audrey eBosse, Ghislaine eHenneke   +3 more
doaj   +1 more source

Probing structural heterogeneities and fluctuations of nucleic acids and denatured proteins [PDF]

Proceedings of the National Academy of Sciences, 2005
We study protein and nucleic acid structure and dynamics using single-molecule FRET and alternating-laser excitation. Freely diffusing molecules are sorted into subpopulations based on single-molecule signals detected within 100 μs to 1 ms.
Laurence, T A, Kong, X X, Jager, M, Weiss, S   +3 more
openaire   +3 more sources

Development of an Interaction Assay between Single-Stranded Nucleic Acids Trapped with Silica Particles and Fluorescent Compounds

Journal of Functional Biomaterials, 2012
Biopolymers are easily denatured by heating, a change in pH or chemical substances when they are immobilized on a substrate. To prevent denaturation of biopolymers, we developed a method to trap a polynucleotide on a substrate by hydrogen bonding using ...
R. Maeda, T. Isoda
doaj   +1 more source

A Rapid and Low-Cost PCR Thermal Cycler for Low Resource Settings. [PDF]

PLoS ONE, 2015
Many modern molecular diagnostic assays targeting nucleic acids are typically confined to developed countries or to the national reference laboratories of developing-world countries.
Grace Wong, Isaac Wong, Kamfai Chan, Yicheng Hsieh, Season Wong   +4 more
doaj   +1 more source

A new approach for detecting HPV DNA in cervical swabs: comparison of nucleic acid extraction with direct PCR

Virology Journal
Background Almost all cases of cervical cancer are associated with persistent high-risk HPV infection. WHO prioritizes primary HPV testing for cervical cancer screening.
Fatih Çubuk, Muhammet Çağrı Yıldız, Fikrican Balcı, Ahmet İrgin, Savaş Aşkın, Ekrem Sağtaş, Sedat kaygusuz   +6 more
doaj   +1 more source

Simplified Paper Format for Detecting HIV Drug Resistance in Clinical Specimens by Oligonucleotide Ligation. [PDF]

PLoS ONE, 2016
Human immunodeficiency virus (HIV) is a chronic infection that can be managed by antiretroviral treatment (ART). However, periods of suboptimal viral suppression during lifelong ART can select for HIV drug resistant (DR) variants.
Nuttada Panpradist, Ingrid A Beck, Michael H Chung, James N Kiarie, Lisa M Frenkel, Barry R Lutz   +5 more
doaj   +1 more source

Novel Selenium-modified Nucleic Acids For Structural and Functional Studies [PDF]

, 2015
Nucleic acids, as one of the most important macromolecules in living systems, play critical roles in storing, transferring, regulating genetic information, directing proteins synthesis, and catalysis. Understanding the structure of nucleic acid can bring
Jiang, Sibo
core   +1 more source

Hypoxanthine-Guanine Phosphoribosyltransferase/adenylate Kinase From Zobellia galactanivorans: A Bifunctional Catalyst for the Synthesis of Nucleoside-5′-Mono-, Di- and Triphosphates

Frontiers in Bioengineering and Biotechnology, 2020
In our search for novel biocatalysts for the synthesis of nucleic acid derivatives, we found a good candidate in a putative dual-domain hypoxanthine-guanine phosphoribosyltransferase (HGPRT)/adenylate kinase (AMPK) from Zobellia galactanivorans (ZgHGPRT ...
Javier Acosta, Jon Del Arco, Maria Luisa Del Pozo, Beliña Herrera-Tapias, Vicente Javier Clemente-Suárez, Vicente Javier Clemente-Suárez, José Berenguer, Aurelio Hidalgo, Jesús Fernández-Lucas, Jesús Fernández-Lucas   +9 more
doaj   +1 more source

Effects of osmolytes and macromolecular crowders on stable GAAA tetraloops and their preference for a CG closing base pair [PDF]

PeerJ, 2018
Osmolytes and macromolecular crowders have the potential to influence the stability of secondary structure motifs and alter preferences for conserved nucleic acid sequences in vivo.
Kaethe N. Leonard, Joshua M. Blose
doaj   +2 more sources