Human PrimPol mutation associated with high myopia has a DNA replication defect [PDF]
, 2014 PrimPol is a primase-polymerase found in humans, and other eukaryotes, involved in bypassing lesions encountered during DNA replication. PrimPol employs both translesion synthesis and repriming mechanisms to facilitate lesion bypass by the replisome ...
Aguilera +27 more
core +1 more source
Cover Picture: Locked Nucleic Acids and Intercalating Nucleic Acids in the Design of Easily Denaturing Nucleic Acids: Thermal Stability Studies (ChemBioChem 12/2004) [PDF]
ChemBioChem, 2004 The cover picture shows fully modified locked nucleic acids (LNAs, orange) and intercalating nucleic acids (INAs®, red) with several insertions of (R)‐1‐O‐(1‐pyrenylmethyl)glycerol, both displaying higher binding affinity to native DNA (white) than to each other. In their full paper on p. 1673 ff, E. B. Pedersen et al.
Vyacheslav V. Filichev +4 more
openaire +1 more source
High-Resolution Detection of Loss of Heterozygosity of Dinucleotide Microsatellite Markers
BioTechniques, 2001 Dinucleotide microsatellite markers are frequently investigated to study inheritance, genetic stability, and allele frequency distribution in a wide variety of genetic disorders. Previous studies have encountered significant problems regarding resolution
Rebecca N. Hourihan +2 more
doaj +1 more source
Development of a nucleic acid lateral flow immunoassay for simultaneous detection of Listeria spp. and Listeriamonocytogenes in food [PDF]
, 2009 We present a new nucleic acid lateral flow immunoassay (NALFIA) for the assessment of listeria contamination. The detection procedure starts with enrichment of sample in Half Fraser broth (24 h). Following isolation of DNA, a duplex PCR is performed with
Amerongen, A., van +3 more
core +2 more sources
A conformational RNA zipper promotes intron ejection during non-conventional XBP1 mRNA splicing. [PDF]
, 2015 The kinase/endonuclease IRE1 is the most conserved signal transducer of the unfolded protein response (UPR), an intracellular signaling network that monitors and regulates the protein folding capacity of the endoplasmic reticulum (ER).
Acosta-Alvear, Diego +3 more
core +1 more source
Easily denaturing nucleic acids derived from intercalating nucleic acids: thermal stability studies, dual duplex invasion and inhibition of transcription start [PDF]
Nucleic Acids Research, 2005 The bulged insertions of (R)-1-O-(pyren-1-ylmethyl)glycerol (monomer P) in two complementary 8mer DNA strands (intercalating nucleic acids) opposite to each other resulted in the formation of an easily denaturing duplex, which had lower thermal stability (21.0 degrees C) than the wild-type double-stranded DNA (dsDNA, 26.0 degrees C), but both modified ...
Filichev, Vyacheslav V +3 more
openaire +3 more sources
Microbiota of Tayohounta, a fermented baobab flavour food of Benin [PDF]
, 2011 The present work provides data on the microbial composition of Tayohounta, a product of natural fermentation of baobab seed kernels. Samples were collected from 3 different small scale producers from Benin at the end of the fermentation process ...
Chadare, F.J. +5 more
core +2 more sources
Development of a combined diphtheria vaccine based on diphtheria anatoxin with microbial adjuvant in the light of modern strategies of vaccines development [PDF]
Annals of Mechnikov's Institute, 2020 The review article is devoted to the consideration of approaches to the practical implementation of the main provisions of modern ideas about the role and mechanisms of innate immunity for the development of vaccines.
Yelyseyeva, I.V. +4 more
doaj +3 more sources
Can we model DNA at the mesoscale ? Comment on: Fluctuations in the DNA double helix: A critical review [PDF]
, 2014 Comment on "Fluctuations in the DNA double helix: A critical review" by Frank-Kamenetskii and ...
Dauxois, Thierry, Peyrard, Michel
core +5 more sources
PCR performance of a thermostable heterodimeric archaeal DNA polymerase
Frontiers in Microbiology, 2014 DNA polymerases are versatile tools used in numerous important molecular biological core technologies like the ubiquitous polymerase chain reaction (PCR), cDNA cloning, genome sequencing and nucleic acid based diagnostics.
Tom eKillelea +3 more
doaj +1 more source