High-Resolution Detection of Loss of Heterozygosity of Dinucleotide Microsatellite Markers
BioTechniques, 2001 Dinucleotide microsatellite markers are frequently investigated to study inheritance, genetic stability, and allele frequency distribution in a wide variety of genetic disorders. Previous studies have encountered significant problems regarding resolution
Rebecca N. Hourihan +2 more
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Partial Denaturation of Double-Stranded DNA on Pristine Graphene under Physiological-like Conditions
Liquids, 2023 Interactions between DNA and graphene are paramount for a wide range of applications, such as biosensing and nanoelectronics; nonetheless, the molecular details of such interactions remain largely unexplored.
Fernando J. A. L. Cruz, José P. B. Mota
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Nucleic acid induced unfolding of recombinant prion protein globular fragment is pH dependent [PDF]
, 2014 Nucleic acid can catalyze the conversion of α-helical cellular prion protein to β-sheet rich Proteinase K resistant prion protein oligomers and amyloid polymers in vitro and in solution.
Bera, Alakesh, Nandi, Pradip
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Cover Picture: Locked Nucleic Acids and Intercalating Nucleic Acids in the Design of Easily Denaturing Nucleic Acids: Thermal Stability Studies (ChemBioChem 12/2004) [PDF]
ChemBioChem, 2004 The cover picture shows fully modified locked nucleic acids (LNAs, orange) and intercalating nucleic acids (INAs®, red) with several insertions of (R)‐1‐O‐(1‐pyrenylmethyl)glycerol, both displaying higher binding affinity to native DNA (white) than to each other. In their full paper on p. 1673 ff, E. B. Pedersen et al.
Vyacheslav V. Filichev +4 more
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PCR performance of a thermostable heterodimeric archaeal DNA polymerase
Frontiers in Microbiology, 2014 DNA polymerases are versatile tools used in numerous important molecular biological core technologies like the ubiquitous polymerase chain reaction (PCR), cDNA cloning, genome sequencing and nucleic acid based diagnostics.
Tom eKillelea +3 more
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Development of a combined diphtheria vaccine based on diphtheria anatoxin with microbial adjuvant in the light of modern strategies of vaccines development [PDF]
Annals of Mechnikov's Institute, 2020 The review article is devoted to the consideration of approaches to the practical implementation of the main provisions of modern ideas about the role and mechanisms of innate immunity for the development of vaccines.
Yelyseyeva, I.V. +4 more
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Journal of Functional Biomaterials, 2012 Biopolymers are easily denatured by heating, a change in pH or chemical substances when they are immobilized on a substrate. To prevent denaturation of biopolymers, we developed a method to trap a polynucleotide on a substrate by hydrogen bonding using ...
R. Maeda, T. Isoda
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Negative Electrospray Supercharging Mechanisms of Nucleic Acid Structures [PDF]
, 2022 International audienceWhen sprayed from physiological ionic strength, nucleic acids typically end up with low levels of charging and in compact conformations.
Valérie Gabelica +6 more
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A Rapid and Low-Cost PCR Thermal Cycler for Low Resource Settings. [PDF]
PLoS ONE, 2015 Many modern molecular diagnostic assays targeting nucleic acids are typically confined to developed countries or to the national reference laboratories of developing-world countries.
Grace Wong +4 more
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Partial molar heat capacities and volumes of some nucleic acid bases, nucleosides and nucleotides in aqueous urea solutions at 298.15 K [PDF]
, 1990 Partial molar heat capacities (C0p2) and volumes (V02) of some nucleic acid bases, nucleosides and nucleotides in 1, 3 and 6 mol kg-1 aqueous urea solutions have been measured using a dynamic-flow microcalorimeter and a vibrating-tube digital densimeter,
Ahluwalia, Jagdish C., Kishore, Nand
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