Results 51 to 60 of about 146,288 (301)

Clinical performance of the urine‐based TERT promoter AbsoluteQ Digital PCR for non‐invasive detection of bladder cancer

Molecular Oncology, EarlyView.
A urine‐based digital PCR assay targeting two hotspot TERT promoter variants detected bladder cancer with high sensitivity and no false positives in this case–control cohort. The streamlined AbsoluteQ workflow outperformed Sanger sequencing and supports non‐invasive molecular testing for bladder cancer detection.
Anna Nykel, Izabela Kubiak, Lena Rutkowska, Żaneta Kasprzyk, Łukasz Kępczyński, Michał Bednarek, Dariusz Sobieraj, Jacek Wilkosz, Piotr Kania, Adam Jędrzejczyk, Bogdan Kałużewski, Agnieszka Gach, Tadeusz Kałużewski   +12 more
wiley   +1 more source

A new approach for detecting HPV DNA in cervical swabs: comparison of nucleic acid extraction with direct PCR

Virology Journal
Background Almost all cases of cervical cancer are associated with persistent high-risk HPV infection. WHO prioritizes primary HPV testing for cervical cancer screening.
Fatih Çubuk, Muhammet Çağrı Yıldız, Fikrican Balcı, Ahmet İrgin, Savaş Aşkın, Ekrem Sağtaş, Sedat kaygusuz   +6 more
doaj   +1 more source

Evidence for conservation in antigen gene sequences combined with extensive polymorphism at VNTR loci [PDF]

, 2020
Theileria parva is a tick‐transmitted apicomplexan protozoan parasite that infects lymphocytes of cattle and African Cape buffalo (Syncerus caffer), causing a frequently fatal disease of cattle in eastern, central and southern Africa.
Ahmed, Jabbar, Bishop, Richard P., Clausen, Peter‐Henning, Kabi, Fredrick, Kasaija, Paul, Lubega, George W., Mwega, Elisa, Nanteza, Anne, Njahira, Moses, Njuguna, Joyce, Obara, Isaiah, Odongo, David, Salih, Diaeldin A., Skilton, Rob A.   +13 more
core   +1 more source

VIVID: A qPCR‐Based Platform for Sensitive and Quantitative In Vivo Tracking of Extracellular Vesicles

Advanced Functional Materials, EarlyView.
This study introduces VIVID (Vesicle In Vivo Identification using DNA), a qPCR‐based platform that tracks PCR‐amplifiable DNA tags loaded in the EVs for accurate and quantifiable EV biodistribution in vivo. ABSTRACT Extracellular vesicles (EVs) represent promising carriers for nucleic acid therapeutics, offering advantages over synthetic nanoparticles ...
Oscar Boyadjian, Talin Ebrahimian, Jonathan Miranda O'Connor, Janusz Rak, Stephanie Lehoux, Maryam Tabrizian   +5 more
wiley   +1 more source

Methods to prevent PCR amplification of DNA from non-viable virus were not successful for infectious laryngotracheitis virus.

PLoS ONE, 2020
Molecular-based testing of poultry dust has been used as a fast, sensitive and specific way to monitor viruses in chicken flocks but it provides no information on viral viability.
Yugal Raj Bindari, Stephen W Walkden-Brown, Priscilla F Gerber   +2 more
doaj   +1 more source

Twist/Writhe Partitioning in a Coarse-Grained DNA Minicircle Model

, 2009
Here we present a systematic study of supercoil formation in DNA minicircles under varying linking number by using molecular dynamics simulations of a two-bead coarse-grained model. Our model is designed with the purpose of simulating long chains without
Alkan Kabakçıoğlu, Barış Avşaroğlu, K. Luger, Mehmet Sayar   +3 more
core   +1 more source

Engineering Extracellular Vesicle Production Through Magnetic Ion Channel Activation for Bone Regeneration

Advanced Healthcare Materials, EarlyView.
Magnetic Ion Channel Activation (MICA) enables remote stimulation of mechanosensitive ion channels using functionalised magnetic nanoparticles, enhancing extracellular vesicle (EV) biogenesis is pre‐osteoblasts. MICA desrived EVs exhibit typical nano‐vesicular characteristics but display superior pro‐osteogeneic activity, promoting mesenchymal stem ...
Afeesh Rajan Unnithan, Kenny Man, Kritika, Lee A. Gethings, Christopher J. Hughes, Alicia Keenan, Liam Heaney, Sophie C. Cox, Owen G. Davies, Alicia J. El Haj   +9 more
wiley   +1 more source

Human plasmacytoid dendritic cells at the crossroad of type I interferon-regulated B cell differentiation and antiviral response to tick-borne encephalitis virus.

PLoS Pathogens, 2021
The Tick-borne encephalitis virus (TBEV) causes different disease symptoms varying from asymptomatic infection to severe encephalitis and meningitis suggesting a crucial role of the human host immune system in determining the fate of the infection. There
Marilena P Etna, Aurora Signorazzi, Daniela Ricci, Martina Severa, Fabiana Rizzo, Elena Giacomini, Andrea Gaggioli, Isabelle Bekeredjian-Ding, Anke Huckriede, Eliana M Coccia   +9 more
doaj   +1 more source

Nanotherapies for Atherosclerosis: Targeting, Catalysis, and Energy Transduction

Advanced Healthcare Materials, EarlyView.
Atherosclerosis management is hindered by poor drug targeting and plaque heterogeneity. Nanotechnology overcomes these barriers via three core strategies: (1) target‐engineered nanocarriers that achieve lesion‐specific precision via ligand modification, biomimetic camouflage, stimuli‐responsive release, and self‐propelling nanomotors; (2) catalytic ...
Yuqi Yang, Jingguo Qu, Tonghuan Xiao, Xinyang Han, Michael Tin‐Cheung Ying   +4 more
wiley   +1 more source

The effects of lidocaine and procaine on microRNA expression of adipocyte-derived adult stem cells [PDF]

Korean Journal of Anesthesiology, 2012
BackgroundThe microRNA (miRNA) pathway has emerged as one of the biologic pathways implicated in stem cell regulation. miRNA is a noncoding, single-stranded RNA consisting of 20-25 nucleotides that inhibits the protein production at the step of ...
Sang Hoon Sung, Jeong Gil Lee, Soo Bong Yu, Hee Kyung Chang, Sie Jeong Ryu   +4 more
doaj   +1 more source