Results 51 to 60 of about 2,650,464 (200)

Cytofluorometric studies on conformation of nucleic acids in situ. II. Denaturation of deoxyribonucleic acid. [PDF]

Journal of Histochemistry & Cytochemistry, 1976
Thermal denaturation of deoxyribonucleic acid (DNA) in situ in individual unbroken cells is studied by a cytofluorometric method. This method allows us to investigate DNA denaturation in the presence of divalent cations at concentrations reported to be necessary to maintain native structure of nuclear chromatin.
Z, Darzynkiewicz, F, Traganos, Z A, Arlin, A T, Sharpless, M R, Melamed   +4 more
openaire   +2 more sources

Functionalized graphene-oxide grids enable high-resolution cryo-EM structures of the SNF2h-nucleosome complex without crosslinking

Nature Communications
Single-particle cryo-EM is widely used to determine enzyme-nucleosome complex structures. However, cryo-EM sample preparation remains challenging and inconsistent due to complex denaturation at the air-water interface (AWI).
Un Seng Chio, Eugene Palovcak, Anton A. A. Smith, Henriette Autzen, Elise N. Muñoz, Zanlin Yu, Feng Wang, David A. Agard, Jean-Paul Armache, Geeta J. Narlikar, Yifan Cheng   +10 more
doaj   +1 more source

Fluorinated peptide nucleic acid [PDF]

, 2003
The fluorinated olefinic peptide nucleic acid analogue (F-OPA) monomer containing the base thymine was synthesised in 13 steps. PNAs containing this unit were prepared and their pairing properties assessed by means of UV-melting ...
Marcel Hollenstein, Gautschi, Daniel, Daniel Gautschi, Leumann, Christian, Hollenstein, Marcel, Christian J. Leumann   +5 more
core   +1 more source

Cover Picture: Locked Nucleic Acids and Intercalating Nucleic Acids in the Design of Easily Denaturing Nucleic Acids: Thermal Stability Studies (ChemBioChem 12/2004) [PDF]

ChemBioChem, 2004
The cover picture shows fully modified locked nucleic acids (LNAs, orange) and intercalating nucleic acids (INAs®, red) with several insertions of (R)‐1‐O‐(1‐pyrenylmethyl)glycerol, both displaying higher binding affinity to native DNA (white) than to each other. In their full paper on p. 1673 ff, E. B. Pedersen et al.
Vyacheslav V. Filichev, Ulf B. Christensen, Erik B. Pedersen, B. Ravindra Babu, Jesper Wengel   +4 more
openaire   +1 more source

Mechanochemistry of nucleosides, nucleotides and related materials

Beilstein Journal of Organic Chemistry, 2018
The application of mechanical force to induce the formation and cleavage of covalent bonds is a rapidly developing field within organic chemistry which has particular value in reducing or eliminating solvent usage, enhancing reaction rates and also in ...
Olga Eguaogie, Joseph S. Vyle, Patrick F. Conlon, Manuela A. Gîlea, Yipei Liang   +4 more
doaj   +1 more source

Insights on the DNA Stability in Aqueous Solutions of Ionic Liquids

Frontiers in Bioengineering and Biotechnology, 2020
Deoxyribonucleic acid (DNA) carries the genetic information essential for the growth and functioning of living organisms, playing a significant role in life sciences research.
Teresa B. V. Dinis, Fani Sousa, Mara G. Freire   +2 more
doaj   +1 more source

Denaturation of HIV-1 Protease (PR) monomer by acetic acid: mechanistic and trajectory insights from molecular dynamics simulations and NMR

, 2012
Inside a living cell there can be a variety of interactions for any given protein, which serve to regulate denaturation and renaturation processes. Insights into some of them can be obtained by in vitro studies using various denaturing agents.
Rout, Manoj Kumar, Hosur, Ramakrishna V., Borkar, Aditi   +2 more
core   +2 more sources

An Extended Dot-Bracket-Notation for Functional Nucleic Acids

, 2008
Functional nucleic acids are an attractive substrate for molecular computing. A nucleic acid molecule is a linear chain of covalently bound building blocks assembled in arbitrary order from a set of typically four nucleotides.
Zauner, Klaus-Peter, Ramlan, Effirul I.
core   +1 more source

Drug-nucleic acid interactions /

, 2001
This volume consolidates the key methods for studying ligand-nucleic acid interactions into a convenient source. Techniques that are examined range from biophysical and chemical approaches to methods rooted in molecular and cell biology.Includes ...
Chaires, Jonathan B.,editor., Waring, Michael J.,editor.   +1 more
core  

The infectivity and inactivation of nucleic acid preparations from tobacco mosaic virus [PDF]

, 1959
The infectivity of nucleic acid preparations made by disrupting tobacco mosaic virus with phenol was increased, relative to that of intact virus, by keeping test plants in darkness or at 37° for some time before they were inoculated.
Pirie, N. W., Bawden, F. C.
core   +1 more source