Results 41 to 50 of about 146,288 (301)
, 2011 Loops are essential secondary structure elements in folded DNA and RNA molecules and proliferate close to the melting transition. Using a theory for nucleic acid secondary structures that accounts for the logarithmic entropy c ln m for a loop of length m,
Einert, Thomas R. +2 more
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Journal of Functional Biomaterials, 2012 Biopolymers are easily denatured by heating, a change in pH or chemical substances when they are immobilized on a substrate. To prevent denaturation of biopolymers, we developed a method to trap a polynucleotide on a substrate by hydrogen bonding using ...
R. Maeda, T. Isoda
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A Rapid and Low-Cost PCR Thermal Cycler for Low Resource Settings. [PDF]
PLoS ONE, 2015 Many modern molecular diagnostic assays targeting nucleic acids are typically confined to developed countries or to the national reference laboratories of developing-world countries.
Grace Wong +4 more
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Cytofluorometric studies on conformation of nucleic acids in situ. II. Denaturation of deoxyribonucleic acid. [PDF]
Journal of Histochemistry & Cytochemistry, 1976 Thermal denaturation of deoxyribonucleic acid (DNA) in situ in individual unbroken cells is studied by a cytofluorometric method. This method allows us to investigate DNA denaturation in the presence of divalent cations at concentrations reported to be necessary to maintain native structure of nuclear chromatin.
Z, Darzynkiewicz +4 more
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Simplified Paper Format for Detecting HIV Drug Resistance in Clinical Specimens by Oligonucleotide Ligation. [PDF]
PLoS ONE, 2016 Human immunodeficiency virus (HIV) is a chronic infection that can be managed by antiretroviral treatment (ART). However, periods of suboptimal viral suppression during lifelong ART can select for HIV drug resistant (DR) variants.
Nuttada Panpradist +5 more
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Effects of osmolytes and macromolecular crowders on stable GAAA tetraloops and their preference for a CG closing base pair [PDF]
PeerJ, 2018 Osmolytes and macromolecular crowders have the potential to influence the stability of secondary structure motifs and alter preferences for conserved nucleic acid sequences in vivo.
Kaethe N. Leonard, Joshua M. Blose
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Twist versus Nonlinear Stacking in Short DNA Molecules
, 2014 The denaturation of the double helix is a template for fundamental biological functions such as replication and transcription involving the formation of local fluctuational openings.
Zoli, Marco
core +1 more source
Frontiers in Bioengineering and Biotechnology, 2020 In our search for novel biocatalysts for the synthesis of nucleic acid derivatives, we found a good candidate in a putative dual-domain hypoxanthine-guanine phosphoribosyltransferase (HGPRT)/adenylate kinase (AMPK) from Zobellia galactanivorans (ZgHGPRT ...
Javier Acosta +9 more
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Physics of base-pairing dynamics in DNA
, 2016 As a key molecule of Life, Deoxyribonucleic acid (DNA) is the focus of numbers of investigations with the help of biological, chemical and physical techniques.
Destainville, Nicolas, Manghi, Manoel
core +3 more sources
Computer programs for analysis of nucleic acid hybridization, thermal denaturation, and gel electrophoresis data [PDF]
Nucleic Acids Research, 1979 Computer programs for the analysis of data from techniques frequently used in nucleic acids research are described. In addition to calculating non-linear, least-squares solutions to equations describing these systems, the programs allow for data editing, normalization, plotting and storage, and are flexible and simple to use.
R F, Murphy, W R, Pearson, J, Bonner
openaire +2 more sources