Results 41 to 50 of about 67,468 (297)

Simplified Paper Format for Detecting HIV Drug Resistance in Clinical Specimens by Oligonucleotide Ligation. [PDF]

PLoS ONE, 2016
Human immunodeficiency virus (HIV) is a chronic infection that can be managed by antiretroviral treatment (ART). However, periods of suboptimal viral suppression during lifelong ART can select for HIV drug resistant (DR) variants.
Nuttada Panpradist, Ingrid A Beck, Michael H Chung, James N Kiarie, Lisa M Frenkel, Barry R Lutz   +5 more
doaj   +1 more source

Hyperactive ice‐binding proteins stabilize cell membranes and improve resistance to dehydration stress in Caenorhabditis elegans

FEBS Open Bio, EarlyView.
TisIBP8, a fungal‐derived hyperactive ice‐binding protein, helps Caenorhabditis elegans survive dehydration. It localizes near cell membranes, reduces cell damage, and helps maintain membrane structure during drying. These results suggest that ice‐binding proteins can protect cells from dehydration stress as well as freezing stress.
Daiki Shimose, Kotaro Ozaki, Ryohei Kuriyama, Yuka Ikemoto, Kazuhiro Mio, Yuji C. Sasaki, Tatsuya Arai, Sakae Tsuda, Yoichi Shinkai, Masahiro Kuramochi   +9 more
wiley   +1 more source

Accurate quantification of nucleic acids using hypochromicity measurements in conjunction with UV spectrophotometry [PDF]

, 2017
UV absorbance spectrophotometry is widely used for the quantification of nucleic acids. For accurate quantification it is important to determine the hypochromocity of the oligonucleotide or complex nucleic acid structure.
David E. Portwood, Nwokeoji, A., Alison O. Nwokeoji, Peter M. Kilby, Kilby, P.M., Mark J. Dickman, Dickman, M.J., Portwood, D.   +7 more
core   +1 more source

A new approach for detecting HPV DNA in cervical swabs: comparison of nucleic acid extraction with direct PCR

Virology Journal
Background Almost all cases of cervical cancer are associated with persistent high-risk HPV infection. WHO prioritizes primary HPV testing for cervical cancer screening.
Fatih Çubuk, Muhammet Çağrı Yıldız, Fikrican Balcı, Ahmet İrgin, Savaş Aşkın, Ekrem Sağtaş, Sedat kaygusuz   +6 more
doaj   +1 more source

Hypoxanthine-Guanine Phosphoribosyltransferase/adenylate Kinase From Zobellia galactanivorans: A Bifunctional Catalyst for the Synthesis of Nucleoside-5′-Mono-, Di- and Triphosphates

Frontiers in Bioengineering and Biotechnology, 2020
In our search for novel biocatalysts for the synthesis of nucleic acid derivatives, we found a good candidate in a putative dual-domain hypoxanthine-guanine phosphoribosyltransferase (HGPRT)/adenylate kinase (AMPK) from Zobellia galactanivorans (ZgHGPRT ...
Javier Acosta, Jon Del Arco, Maria Luisa Del Pozo, Beliña Herrera-Tapias, Vicente Javier Clemente-Suárez, Vicente Javier Clemente-Suárez, José Berenguer, Aurelio Hidalgo, Jesús Fernández-Lucas, Jesús Fernández-Lucas   +9 more
doaj   +1 more source

Effects of osmolytes and macromolecular crowders on stable GAAA tetraloops and their preference for a CG closing base pair [PDF]

PeerJ, 2018
Osmolytes and macromolecular crowders have the potential to influence the stability of secondary structure motifs and alter preferences for conserved nucleic acid sequences in vivo.
Kaethe N. Leonard, Joshua M. Blose
doaj   +2 more sources

Blood RNA Biomarker Signatures for Early Diagnosis and Prognosis in Ischemic and Hemorrhagic Stroke: The IBIS‐CT1 Study

Annals of Clinical and Translational Neurology, EarlyView.
ABSTRACT Objective To evaluate the expression of nine blood RNA biomarkers in a clinical trial based on genes previously identified in an experimental monkey model of stroke for diagnosis feasibility and prognostication. Methods IBIS‐CT1 was a prospective longitudinal study enrolling patients with ischemic stroke (IS) or intracerebral hemorrhage (ICH ...
Salomé Retailleau, Marie Bruguet, Irina Viakhireva‐Dovganyuk, François‐Mathias Merrien, Denis Marechal, Aurore Jourdain, Philippe Goas, Jordan Coris, Julien Asselineau, Maëlys Consigny, Gérald Le Gac, Serge Timsit   +11 more
wiley   +1 more source

Methods to prevent PCR amplification of DNA from non-viable virus were not successful for infectious laryngotracheitis virus.

PLoS ONE, 2020
Molecular-based testing of poultry dust has been used as a fast, sensitive and specific way to monitor viruses in chicken flocks but it provides no information on viral viability.
Yugal Raj Bindari, Stephen W Walkden-Brown, Priscilla F Gerber   +2 more
doaj   +1 more source

Easily denaturing nucleic acids derived from intercalating nucleic acids: thermal stability studies, dual duplex invasion and inhibition of transcription start [PDF]

Nucleic Acids Research, 2005
The bulged insertions of (R)-1-O-(pyren-1-ylmethyl)glycerol (monomer P) in two complementary 8mer DNA strands (intercalating nucleic acids) opposite to each other resulted in the formation of an easily denaturing duplex, which had lower thermal stability (21.0 degrees C) than the wild-type double-stranded DNA (dsDNA, 26.0 degrees C), but both modified ...
Filichev, Vyacheslav V, Vester, Birte, Hansen, Lykke Haastrup, Pedersen, Erik B   +3 more
openaire   +3 more sources

Lonicera japonica‐Derived Nanovesicles Ameliorate Acute Lung Injury by Suppressing Neutrophil Extracellular Traps Formation in Mice

Advanced Healthcare Materials, EarlyView.
(A) Isolation and purification of LDNVs. (B) Establishment of the ALI model and LDNVs delivery. (C) Therapeutic mechanisms of LDNVs in treating ALI. This scheme figure was drawn by Figdraw, ID: IUIIO21222. ABSTRACT Acute lung injury (ALI) is a lethal respiratory disease.
Xi Lin, Jian Lin, Yezi Zhang, Xinyan Zhang, Xinghua Liu, Hongying Cao, Huan Shen, Jianming Liang, Zhenhua Dai, Siyuan Xu, Xingdong Lin   +10 more
wiley   +1 more source