Results 21 to 30 of about 67,468 (297)
Microgels and apparatus for PAGE of nucleic acids in one or two dimensions
BioTechniques, 2018 We describe a system for horizontal 1D or 2D PAGE comprising an apparatus and microgels. There is no buffer outside the gel, making handling and sample loading easy.
Hans G Thormar +5 more
doaj +1 more source
Emergent Properties and Functions of Nanoconfined Nucleic Acid Architectures [PDF]
, 2015 The facile ability of DNA to self-assemble has enabled the creation of complex architectures with diverse functions on surfaces or in solution. This approach provides a powerful design tool for the development of nanoscale devices with transformative ...
Lucia Coral +11 more
core +1 more source
Cold Denaturation of the Hammerhead Ribozyme [PDF]
, 2016 Cold denaturation is a thermodynamic phenomenon resulting from a difference in the heat capacities, ΔCp, of the folded and unfolded states of a macromolecule.
Peter J. Mikulecky (2716048) +1 more
core +3 more sources
Synergistic effects between analogs of DNA and RNA improve the potency of siRNA-mediated gene silencing [PDF]
, 2010 We report that combining a DNA analog (2?F-ANA) with rigid RNA analogs [2?F-RNA and/or locked nucleic acid (LNA)] in siRNA duplexes can produce gene silencing agents with enhanced potency. The favored conformations of these two analogs are different, and
Alain, Tommy +9 more
core +1 more source
Molecules, 2014 A new photoisomeric nucleoside dUAz bearing an azobenzene group at the C5-position of 2'-deoxyuridine was designed and synthesized. Photoisomerization of dUAz in oligodeoxynucleotides can be achieved rapidly and selectively with 365 nm (forward) and 450
Shohei Mori +2 more
doaj +1 more source
A Widely Applicable Urea-based Fluorescent/Colorimetric mRNA in situ Hybridization Protocol
Bio-Protocol, 2019 In situ hybridization methods are routinely employed to detect nucleic acid sequences, allowing to localize gene expression or to study chromosomal organization in their native context. These methods rely on the pairwise binding of a labeled probe to the
Chiara Sinigaglia
doaj +1 more source
Novel insights into the use of Glowing LNA as nucleic acid detection probes—Influence of labeling density and nucleobases [PDF]
, 2010 Appropriately designed 2′-N-(pyren-1-yl)carbonyl-2′-amino-LNA (locked nucleic acid) display large increases in fluorescence intensity and remarkably high quantum yields upon hybridization with nucleic acid targets.
Jesper Wengel +9 more
core +1 more source
Orthopaedic Surgery, 2022 Objective Pre‐implantation sterilization procedures for tendons are important measures to reduce the risk of disease transmission, however these procedures may compromise tendon microarchitecture and biomechanical properties to varying degrees.
Hao‐ran Zhang +7 more
doaj +1 more source
Biosensors, 2012 Although real-time PCR (RT-PCR) has become a diagnostic standard for rapid identification of bacterial species, typical methods remain time-intensive due to sample preparation and amplification cycle times.
Maxim Shusteff +2 more
doaj +1 more source
An improved nucleic acid sequence-based amplification method mediated by T4 gene 32 protein.
PLoS ONE, 2022 The uptake of Nucleic Acid Sequence-Based Amplification (NASBA) for point of care testing may be hindered by a complexity in the workflow due the requirement of a thermal denaturation step to initiate the cyclic isothermal amplification before the ...
Yi Heng Nai +2 more
doaj +1 more source