Results 21 to 30 of about 2,650,464 (200)
Design, synthesis, conformational analysis and nucleic acid hybridisation properties of thymidyl pyrrolidine-amide oligonucleotide mimics (POM) [PDF]
, 2003 Pyrrolidine-amide oligonucleotide mimics (POM) 1 were designed to be stereochemically and conformationally similar to natural nucleic acids, but with an oppositely charged, cationic backbone.
Tan, T. H. Samuel +14 more
core +1 more source
Molecules, 2014 A new photoisomeric nucleoside dUAz bearing an azobenzene group at the C5-position of 2'-deoxyuridine was designed and synthesized. Photoisomerization of dUAz in oligodeoxynucleotides can be achieved rapidly and selectively with 365 nm (forward) and 450
Shohei Mori +2 more
doaj +1 more source
Denaturation of proteins and nucleic acids by thermal-gradient electrophoresis [PDF]
Biochemical Journal, 1981 A polyacrylamide-gel-electrophoresis method has been developed that permits the analysis of conformational changes that occur during the thermal denaturation of macromolecules. A stable transverse temperature gradient was produced in an aluminium heating jacket clamped around a vertical polyacrylamide slab gel.
D R, Thatcher, B, Hodson
openaire +2 more sources
A Widely Applicable Urea-based Fluorescent/Colorimetric mRNA in situ Hybridization Protocol
Bio-Protocol, 2019 In situ hybridization methods are routinely employed to detect nucleic acid sequences, allowing to localize gene expression or to study chromosomal organization in their native context. These methods rely on the pairwise binding of a labeled probe to the
Chiara Sinigaglia
doaj +1 more source
Biosensors, 2012 Although real-time PCR (RT-PCR) has become a diagnostic standard for rapid identification of bacterial species, typical methods remain time-intensive due to sample preparation and amplification cycle times.
Maxim Shusteff +2 more
doaj +1 more source
Synthesis and Evaluation of Artificial Nucleic Acid Bearing an Oxanorbornane Scaffold
Molecules, 2020 Natural oligonucleotides have many rotatable single bonds, and thus their structures are inherently flexible. Structural flexibility leads to an entropic loss when unwound oligonucleotides form a duplex with single-stranded DNA or RNA.
Hibiki Komine +8 more
doaj +1 more source
Orthopaedic Surgery, 2022 Objective Pre‐implantation sterilization procedures for tendons are important measures to reduce the risk of disease transmission, however these procedures may compromise tendon microarchitecture and biomechanical properties to varying degrees.
Hao‐ran Zhang +7 more
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Crystal structure of a DNA containing the planar, phenoxazine-derived bi-functional spectroscopic probe Ç [PDF]
, 2011 Electron Paramagnetic Resonance (EPR) spectroscopy and fluorescence spectroscopy are complementary biophysical techniques used to examine the structure and dynamics of macromolecules.
Reginsson, Gunnar Widtfeldt +13 more
core +1 more source
High-Resolution Detection of Loss of Heterozygosity of Dinucleotide Microsatellite Markers
BioTechniques, 2001 Dinucleotide microsatellite markers are frequently investigated to study inheritance, genetic stability, and allele frequency distribution in a wide variety of genetic disorders. Previous studies have encountered significant problems regarding resolution
Rebecca N. Hourihan +2 more
doaj +1 more source
An improved nucleic acid sequence-based amplification method mediated by T4 gene 32 protein.
PLoS ONE, 2022 The uptake of Nucleic Acid Sequence-Based Amplification (NASBA) for point of care testing may be hindered by a complexity in the workflow due the requirement of a thermal denaturation step to initiate the cyclic isothermal amplification before the ...
Yi Heng Nai +2 more
doaj +1 more source