A Widely Applicable Urea-based Fluorescent/Colorimetric mRNA in situ Hybridization Protocol
Bio-Protocol, 2019 In situ hybridization methods are routinely employed to detect nucleic acid sequences, allowing to localize gene expression or to study chromosomal organization in their native context. These methods rely on the pairwise binding of a labeled probe to the
Chiara Sinigaglia
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Orthopaedic Surgery, 2022 Objective Pre‐implantation sterilization procedures for tendons are important measures to reduce the risk of disease transmission, however these procedures may compromise tendon microarchitecture and biomechanical properties to varying degrees.
Hao‐ran Zhang +7 more
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Biosensors, 2012 Although real-time PCR (RT-PCR) has become a diagnostic standard for rapid identification of bacterial species, typical methods remain time-intensive due to sample preparation and amplification cycle times.
Maxim Shusteff +2 more
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An improved nucleic acid sequence-based amplification method mediated by T4 gene 32 protein.
PLoS ONE, 2022 The uptake of Nucleic Acid Sequence-Based Amplification (NASBA) for point of care testing may be hindered by a complexity in the workflow due the requirement of a thermal denaturation step to initiate the cyclic isothermal amplification before the ...
Yi Heng Nai +2 more
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High-Resolution Detection of Loss of Heterozygosity of Dinucleotide Microsatellite Markers
BioTechniques, 2001 Dinucleotide microsatellite markers are frequently investigated to study inheritance, genetic stability, and allele frequency distribution in a wide variety of genetic disorders. Previous studies have encountered significant problems regarding resolution
Rebecca N. Hourihan +2 more
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Partial Denaturation of Double-Stranded DNA on Pristine Graphene under Physiological-like Conditions
Liquids, 2023 Interactions between DNA and graphene are paramount for a wide range of applications, such as biosensing and nanoelectronics; nonetheless, the molecular details of such interactions remain largely unexplored.
Fernando J. A. L. Cruz, José P. B. Mota
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Cover Picture: Locked Nucleic Acids and Intercalating Nucleic Acids in the Design of Easily Denaturing Nucleic Acids: Thermal Stability Studies (ChemBioChem 12/2004) [PDF]
ChemBioChem, 2004 The cover picture shows fully modified locked nucleic acids (LNAs, orange) and intercalating nucleic acids (INAs®, red) with several insertions of (R)‐1‐O‐(1‐pyrenylmethyl)glycerol, both displaying higher binding affinity to native DNA (white) than to each other. In their full paper on p. 1673 ff, E. B. Pedersen et al.
Vyacheslav V. Filichev +4 more
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Single-molecule chemical denaturation of riboswitches
, 2013 To date, single-molecule RNA science has been developed almost exclusively around the effect of metal ions as folding promoters and stabilizers of the RNA structure.
St-Pierre, Patrick +14 more
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Development of a combined diphtheria vaccine based on diphtheria anatoxin with microbial adjuvant in the light of modern strategies of vaccines development [PDF]
Annals of Mechnikov's Institute, 2020 The review article is devoted to the consideration of approaches to the practical implementation of the main provisions of modern ideas about the role and mechanisms of innate immunity for the development of vaccines.
Yelyseyeva, I.V. +4 more
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PCR performance of a thermostable heterodimeric archaeal DNA polymerase
Frontiers in Microbiology, 2014 DNA polymerases are versatile tools used in numerous important molecular biological core technologies like the ubiquitous polymerase chain reaction (PCR), cDNA cloning, genome sequencing and nucleic acid based diagnostics.
Tom eKillelea +3 more
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