A Widely Applicable Urea-based Fluorescent/Colorimetric mRNA in situ Hybridization Protocol
Bio-Protocol, 2019 In situ hybridization methods are routinely employed to detect nucleic acid sequences, allowing to localize gene expression or to study chromosomal organization in their native context. These methods rely on the pairwise binding of a labeled probe to the
Chiara Sinigaglia
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Structure, kinetic characterization and subcellular localization of the two ribulose 5-phosphate epimerase isoenzymes from Trypanosoma cruzi [PDF]
, 2017 The enzyme of the pentose phosphate pathway (PPP) ribulose-5-phosphate-epimerase (RPE) is encoded by two genes present in the genome of Trypanosoma cruzi CL Brener clone: TcRPE1 and TcRPE2. Despite high sequence similarity at the amino acid residue level,
Cazzulo, Juan Jose +4 more
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Orthopaedic Surgery, 2022 Objective Pre‐implantation sterilization procedures for tendons are important measures to reduce the risk of disease transmission, however these procedures may compromise tendon microarchitecture and biomechanical properties to varying degrees.
Hao‐ran Zhang +7 more
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Rubredoxin Variant Folds without Iron [PDF]
, 1999 Pyroccocus furiosus rubredoxin (PFRD), like most studied hyperthermophilic proteins, does not undergo reversible folding. The irreversibility of folding is thought to involve PFRD’s iron-binding site.
Mayo, Stephen L., Strop, Pavel
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A pipeline for targeted metagenomics of environmental bacteria. [PDF]
, 2020 BackgroundMetagenomics and single cell genomics provide a window into the genetic repertoire of yet uncultivated microorganisms, but both methods are usually taxonomically untargeted.
Bowers, Robert M +7 more
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Synthesis and Evaluation of Artificial Nucleic Acid Bearing an Oxanorbornane Scaffold
Molecules, 2020 Natural oligonucleotides have many rotatable single bonds, and thus their structures are inherently flexible. Structural flexibility leads to an entropic loss when unwound oligonucleotides form a duplex with single-stranded DNA or RNA.
Hibiki Komine +8 more
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An improved nucleic acid sequence-based amplification method mediated by T4 gene 32 protein.
PLoS ONE, 2022 The uptake of Nucleic Acid Sequence-Based Amplification (NASBA) for point of care testing may be hindered by a complexity in the workflow due the requirement of a thermal denaturation step to initiate the cyclic isothermal amplification before the ...
Yi Heng Nai +2 more
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Measurement of Epstein-Barr virus DNA load using a novel quantification standard containing two EBV DNA targets and SYBR Green I dye [PDF]
, 2016 BACKGROUND Reactivation of Epstein-Barr virus (EBV) infection may cause serious, life-threatening complications in immunocompromised individuals. EBV DNA is often detected in EBV-associated disease states, with viral load believed to be a reflection of ...
Ausimmune Investigator Group (AIG) +6 more
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Biosensors, 2012 Although real-time PCR (RT-PCR) has become a diagnostic standard for rapid identification of bacterial species, typical methods remain time-intensive due to sample preparation and amplification cycle times.
Maxim Shusteff +2 more
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Partial Denaturation of Double-Stranded DNA on Pristine Graphene under Physiological-like Conditions
Liquids, 2023 Interactions between DNA and graphene are paramount for a wide range of applications, such as biosensing and nanoelectronics; nonetheless, the molecular details of such interactions remain largely unexplored.
Fernando J. A. L. Cruz, José P. B. Mota
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