Results 11 to 20 of about 67,253 (293)
Unlocked nucleic acid (UNA) and UNA derivatives: Thermal denaturation studies [PDF]
Nucleic Acids Symposium Series, 2008 A study on the thermal stability of duplexes formed between unlocked nucleic acid (UNA) modified DNA and RNA oligonucleotides and complementary DNA and RNA is presented. The acyclic UNA monomers are shown to induce a decrease in duplex thermal stability.
Jensen, Troels Bundgaard +2 more
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Cytofluorometric studies on conformation of nucleic acids in situ. II. Denaturation of deoxyribonucleic acid. [PDF]
Journal of Histochemistry & Cytochemistry, 1976 Thermal denaturation of deoxyribonucleic acid (DNA) in situ in individual unbroken cells is studied by a cytofluorometric method. This method allows us to investigate DNA denaturation in the presence of divalent cations at concentrations reported to be necessary to maintain native structure of nuclear chromatin.
Z, Darzynkiewicz +4 more
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Nucleic acid binding and other biomedical properties of artificial oligolysines
International Journal of Nanomedicine, 2016 Giovanni N Roviello,1 Caterina Vicidomini,1 Vincenzo Costanzo,1 Valentina Roviello2 1CNR Istituto di Biostrutture e Bioimmagini, Via Mezzocannone site and Headquarters, 2Centro Regionale di Competenza (CRdC) Tecnologie, Via Nuova Agnano, Napoli, Italy ...
Roviello GN +3 more
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Synthesis and Evaluation of Artificial Nucleic Acid Bearing an Oxanorbornane Scaffold
Molecules, 2020 Natural oligonucleotides have many rotatable single bonds, and thus their structures are inherently flexible. Structural flexibility leads to an entropic loss when unwound oligonucleotides form a duplex with single-stranded DNA or RNA.
Hibiki Komine +8 more
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Fabrication of Silver Nanoparticles Against Fungal Pathogens
Frontiers in Nanotechnology, 2021 The use of silver nanoparticles (AgNPs) against various pathogens is now being well recognized in the agriculture and health sector. Nanoparticles have been shown to exhibit various novel properties and these properties, on other hand, rely upon the size,
Sheikh Mansoor +6 more
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Atomic scissors: a new method of tracking the 5-bromo-2'-deoxyuridine-labeled DNA in situ. [PDF]
PLoS ONE, 2012 A new method of the light microscopy detection of BrdU-labeled DNA in situ is described. It is based on the oxidative attack at the deoxyribose moiety by copper(I) in the presence of oxygen, which leads to the abstraction of hydrogen atom from ...
Anna Ligasová +4 more
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TNOA Journal of Ophthalmic Science and Research, 2021 Polymerase chain reaction (PCR) is a revolutionary innovation involving enzymatic amplification of nucleic acid sequences in repeated cycles of denaturation, oligonucleotide annealing, and DNA polymerase extension.
Arkaprava Pradhan +2 more
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Probing structural heterogeneities and fluctuations of nucleic acids and denatured proteins [PDF]
Proceedings of the National Academy of Sciences, 2005 We study protein and nucleic acid structure and dynamics using single-molecule FRET and alternating-laser excitation. Freely diffusing molecules are sorted into subpopulations based on single-molecule signals detected within 100 μs to 1 ms.
Laurence, T A +3 more
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Microgels and apparatus for PAGE of nucleic acids in one or two dimensions
BioTechniques, 2018 We describe a system for horizontal 1D or 2D PAGE comprising an apparatus and microgels. There is no buffer outside the gel, making handling and sample loading easy.
Hans G Thormar +5 more
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Molecules, 2014 A new photoisomeric nucleoside dUAz bearing an azobenzene group at the C5-position of 2'-deoxyuridine was designed and synthesized. Photoisomerization of dUAz in oligodeoxynucleotides can be achieved rapidly and selectively with 365 nm (forward) and 450
Shohei Mori +2 more
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