SmallTalk: a novel small‐sized fusion tag for peptide expression and purification
The SmallTalk fusion tag allows for the efficient expression and purification of soluble recombinant proteins or peptides in Escherichia coli. Testing with SmallTalk‐GFP confirmed that the proteins were soluble and folded correctly, while SmallTalk‐Bin1b maintained its antimicrobial activity against various bacterial isolates. This streamlined workflow
Atika Tariq +3 more
wiley +1 more source
Branchpoint Expansion in a Fully Complementary Three-Way DNA Junction
Tara Sabir +6 more
openalex +2 more sources
DDX3X induces mesenchymal transition of endothelial cells by disrupting BMPR2 signaling
Elevated DDX3X expression led to downregulation of BMPR2, a key regulator of endothelial homeostasis and function. Our co‐immunoprecipitation assays further demonstrated a molecular interaction between DDX3X and BMPR2. Notably, DDX3X promoted lysosomal degradation of BMPR2, thereby impairing its downstream signaling and facilitating endothelial‐to ...
Yu Zhang +7 more
wiley +1 more source
Regulation of Duplex Formation in Response to pH and Complementary RNA: Development of Oligonucleotides Modified with <i>S</i>‑Methylthioimidate-Bridged Nucleic Acid and Evaluation of Their Function. [PDF]
Osawa T +4 more
europepmc +1 more source
Deoxyribonucleic Acid (DNA) Computing using Two-by-Six Complementary and Color Code Cipher
B. Adithya, G. Santhi
openalex +1 more source
ATP13A2 is involved in intracellular polyamine transport in lung epithelial cells
Spermidine transport in lung epithelial cells involves the polyamine transporter ATP13A2. Cell proliferation is associated with the upregulation of ATP13A2. Polyamines are present in all living cells and are implicated in various crucial cellular processes such as proliferation, apoptosis and autophagy.
Yuta Hatori +8 more
wiley +1 more source
Activation of enzymatic catalysis via nucleic acid hybridization affords synergistic coupling of specificity, potency, and signal amplification. [PDF]
Montasell MC +4 more
europepmc +1 more source
RoboMic is an automated confocal microscopy pipeline for high‐throughput functional imaging in living cells. Demonstrated with fluorescence recovery after photobleaching (FRAP), it integrates AI‐driven nuclear segmentation, ROI selection, bleaching, and analysis.
Selçuk Yavuz +6 more
wiley +1 more source
Gamma-FIT-PNAs as sensitive RNA probes. [PDF]
Gupta MK, Maree S, Yavin E.
europepmc +1 more source

