Results 81 to 90 of about 3,270,422 (387)

Quantification of DNA by a Thermal-Durable Biosensor Modified with Conductive Poly(3,4-ethylenedioxythiophene)

Sensors, 2018
The general clinical procedure for viral DNA detection or gene mutation diagnosis following polymerase chain reaction (PCR) often involves gel electrophoresis and DNA sequencing, which is usually time-consuming.
Yesong Gu, Po-Yuan Tseng, Xiang Bi, Jason H. C. Yang   +3 more
doaj   +1 more source

Structural Landscape of the Transition from an ssDNA Dumbbell Plus Its Complementary Hairpin to a dsDNA Microcircle Via a Kissing Loop Intermediate

Molecules, 2021
The experimental construction of a double-stranded DNA microcircle of only 42 base pairs entailed a great deal of ingenuity and hard work. However, figuring out the three-dimensional structures of intermediates and the final product can be particularly ...
Alberto Mills, Federico Gago
doaj   +1 more source

The Escherichia coli RutR transcription factor binds at targets within genes as well as intergenic regions. [PDF]

, 2008
The Escherichia coli RutR protein is the master regulator of genes involved in pyrimidine catabolism. Here we have used chromatin immunoprecipitation in combination with DNA microarrays to measure the binding of RutR across the chromosome of ...
Akira Ishihama, Baba, Babu, Babu, Browning, Cho, Datsenko, David C. Grainger, Efromovich, Grainger, Grainger, Grainger, Grainger, Hiard, Li, Loh, Martinez-Antonio, Pavesi, Robison, Shimada, Stephen J. W. Busby, Tomohiro Shimada, Wade, Wade, Wei   +24 more
core   +5 more sources

Protonophore activity of short‐chain fatty acids induces their intracellular accumulation and acidification

FEBS Letters, EarlyView.
The protonated form of butyrate, as well as other short‐chain fatty acids (SCFAs), is membrane permeable. In acidic extracellular environments, this can lead to intracellular accumulation of SCFAs and cytosolic acidification. This phenomenon will be particularly relevant in acidic environments such as the large intestine or tumor microenvironments ...
Muwei Jiang, Frans Bianchi, Geert van den Bogaart   +2 more
wiley   +1 more source

A cellulose-based bioassay for the colorimetric detection of pathogen DNA [PDF]

, 2014
Cellulose-paper-based colorimetric bioassays may be used at the point of sampling without sophisticated equipment. This study reports the development of a colorimetric bioassay based on cellulose that can detect pathogen DNA.
Goyal, Madhu, Kukol, A., Rossiter, Sharon, Saikrishnan, Deepika   +3 more
core   +1 more source

A stepwise emergence of evolution in the RNA world

FEBS Letters, EarlyView.
How did biological evolution emerge from chemical reactions? This perspective proposes a gradual scenario of self‐organization among RNA molecules, where catalytic feedback on random mixtures plays the central role. Short oligomers cross‐ligate, and self‐assembly enables heritable variations. An event of template‐externalization marks the transition to
Philippe Nghe
wiley   +1 more source

Development of a Fluorescence Resonance Energy Transfer (FRET)-Based DNA Biosensor for Detection of Synthetic Oligonucleotide of Ganoderma boninense

Biosensors, 2013
An optical DNA biosensor based on fluorescence resonance energy transfer (FRET) utilizing synthesized quantum dot (QD) has been developed for the detection of specific-sequence of DNA for Ganoderma boninense, an oil palm pathogen.
Noremylia Mohd Bakhori, Nor Azah Yusof, Abdul Halim Abdullah, Mohd Zobir Hussein   +3 more
doaj   +1 more source

Deletion mutants of bacteriophage phiX174 [PDF]

, 1972
Mutants of bacteriophage phiX174 have been isolated that are less dense than wild-type phiX particles in CsCl. When mutant viral (+) strand DNA and wild-type complementary (-) strand DNA are hybridized, the resulting duplex molecules have single-stranded
Benbow, Robert M., Sinsheimer, Robert L., Zuccarelli, Anthony J.   +2 more
core  

Molecular Spiders with Memory [PDF]

, 2007
Synthetic bio-molecular spiders with "legs" made of single-stranded segments of DNA can move on a surface which is also covered by single-stranded segments of DNA complementary to the leg DNA.
G. H. Weiss, I. Benjamini, J. Howard, M. P. W. Zerner, N. G. Van Kampen, P. L. Krapivsky, S. Klumpp, S. Redner, Tibor Antal   +8 more
core   +1 more source

Modifications in FLAP's second cytosolic loop influence 5‐LOX interaction, inhibitor binding, and leukotriene formation

FEBS Letters, EarlyView.
The enzyme 5‐lipoxygenase (5‐LOX) catalyzes the first step in the biosynthesis of leukotrienes (LTs) involved in inflammatory pathophysiology. After cellular stimulation, 5‐LOX translocates to the nucleus, interacting with the 5‐LOX‐activating protein (FLAP) to form LTA4 from arachidonic acid (AA).
Erik Romp, Katharina Rataj, Stefanie König, Marcia E. Newcomer, Oliver Werz, Ulrike Garscha   +5 more
wiley   +1 more source