Results 121 to 130 of about 394,134 (317)

Using real options to select stable Middleware-induced software architectures

, 2005
The requirements that force decisions towards building distributed system architectures are usually of a non-functional nature. Scalability, openness, heterogeneity, and fault-tolerance are examples of such non-functional requirements.
W. Emmerich, J. Macke, R. Bahsoon, Bahsoon, R., Macke, J., Emmerich, W.   +5 more
core   +1 more source

PERSPECTIVE ARCHITECTURE AND COMPONENTS OF COMPUTER NETWORKS

International Journal of Computing, 2004
This article is about using perspective architecture and components of computer networks will permit to increase productivity and reliability of the specialized computer networks not at the expense of escalating elements of system.
Yaroslav Nykolaychuk, Nazar Krutskevych, Oleg Zastavniy, Taras Grinchyshyn   +3 more
openaire   +2 more sources

Single‐molecule DNA flow‐stretch assays for high‐throughput DNA–protein interaction studies

FEBS Open Bio, EarlyView.
We describe an optimised single‐molecule DNA flow‐stretch assay that visualises DNA–protein interactions in real time. Linear DNA fragments are tethered to a surface and stretched by buffer flow for fluorescence imaging. Using λ and φX174 DNA, this protocol enhances reproducibility and accessibility, providing a versatile approach for studying diverse ...
Ayush Kumar Ganguli, Mohammad Nour Alsamsam, Ugnė Bagdonaitė, Van Truc Vu, Chun‐Jen Huang, Polina Kuzhir, Mindaugas Zaremba, Aurimas Kopūstas, Marijonas Tutkus   +8 more
wiley   +1 more source

Screening and epitope characterization of Nidogen‐2‐specific nanobodies

FEBS Open Bio, EarlyView.
Camel immunization and phage display were employed to generate high‐affinity VHH nanobodies against Nidogen‐2. After library construction, biopanning, ELISA screening, sequencing, and recombinant expression, selected nanobodies were purified and characterized, leading to the preliminary exploration of a nanobody‐based sandwich ELISA for specific ...
Jianchuan Wen, Qianqian Cui, Zhongyun Lan, Yingjun Wang, Shuaiying Zhao, Wenxuan Feng, Yunfeng Liu, Qiting Huang, Dongna Zhang, Jianfeng Xu   +9 more
wiley   +1 more source

Evolutionarily divergent DUF4465 domains have a common vitamin B12‐binding function

FEBS Open Bio, EarlyView.
We show that DUF4465 family proteins, widespread across bacteria from gut microbiomes, hydrothermal vents, and soil, share a common vitamin B12‐binding function. These augmented β‐jellyroll proteins bind vitamin B12 via extended loops. Our findings establish sequence‐diverse DUF4465 proteins as a widespread class of B12‐binding proteins, highlighting ...
Charlea Clarke, Michal Banasik, Rokas Juodeikis, Martin J. Warren, Richard W. Pickersgill   +4 more
wiley   +1 more source

A Web Services Component Discovery and Deployment Architecture for Simulation Model Reuse

, 2006
CSPs are widely used in industry, although have yet to operate across organizational boundaries. Reuse across organizations is restricted by the same semantic issues that restrict the inter-organization use of web services. The current representations of
Lycett, M, Bell, D, Mustafee, N, de Cesare, S, Taylor, S J E   +4 more
core  

TINA as a virtual market place for telecommunication and information services: the VITAL experiment

, 1999
The VITAL (Validation of Integrated Telecommunication Architectures for the Long-Term) project has defined, implemented and demonstrated an open distributed telecommunication architecture (ODTA) for deploying, managing and using a set of heterogeneous ...
Lago, Patricia, Canal, G., T. Mota, T. Tiropanis, P. Lago, MOTA T., G. Canal, Telma, Mota, Hellemans, Patrick, Tiropanis, Thanassis, P. Hellemans, Hellemans, P., Canal, Gianni, Tiropanis, T., Mota, T., TIROPANIS T., CANAL G., HELLEMANS P.   +17 more
core   +1 more source

Architecture, component, and microbiome of biofilm involved in the fouling of membrane bioreactors

npj Biofilms and Microbiomes, 2017
Bioreactors: Films affecting filtration A study of factors affecting the accumulation of biofilms on filtration membranes in bioreactors should aid efforts to combat the problem.
Tomohiro Inaba, Tomoyuki Hori, Hidenobu Aizawa, Atsushi Ogata, Hiroshi Habe   +4 more
doaj   +1 more source

Mass spectrometry based identification of AMP‐O‐Tris generated by Thermococcus onnurineus Cas10

FEBS Open Bio, EarlyView.
Isolated Thermococcus onnurineus Cas10 generates the noncanonical ATP‐derived product AMP‐O‐Tris while in Tris‐containing buffer as identified via mass spectrometry, revealing relaxed nucleophile selectivity under isolated conditions. These findings suggest that multiprotein Csm complex assembly restricts Cas10 reactivity toward canonical cyclic ...
Su‐Jin Lee, Ga Seul Lee, JinHan Kim, Kwang‐Hyun Park, Seong‐Ryung Go, Jeong Hee Moon, Eui‐Jeon Woo   +6 more
wiley   +1 more source

Transforming Platform-Independent to Platform-Specific Component and Connector Software Architecture Models

, 2022
3035Combining component & connector architecture description languages with component behavior modeling languages enables modeling great parts of software architectures platform independently.
Rumpe, B., Ringert, J.O., Wortmann, A.
core