Results 31 to 40 of about 99,383 (266)

Calpain small subunit homodimerization is robust and calcium‐independent

FEBS Letters, EarlyView.
Calpains dimerize via penta‐EF‐hand (PEF) domains. Using single‐molecule force spectroscopy, we measured the strength and kinetics of PEF–PEF homodimer binding. The interaction is robust, shows a transient conformational step before dissociation, and remains largely insensitive to Ca2+.
Nesha May O. Andoy, Trina Dykstra‐MacPherson, Mathias A. Bell, Peter L. Davies, Ruby May A. Sullan   +4 more
wiley   +1 more source

Failure Assessment of Steel-Concrete Composite Column Under Blast Loading

Engineering Transactions, 2014
Composite column as a key structural member can be subjected to a blast load as a result of an accident or a terrorist threat. In this paper, a method for assessing the blast resistance of a composite concrete-filled column is proposed.
Marcin P. BUDZIAK, Tomasz GARBOWSKI
doaj   +1 more source

Structural insights into an engineered feruloyl esterase with improved MHET degrading properties

FEBS Letters, EarlyView.
A feruloyl esterase was engineered to mimic key features of MHETase, enhancing the degradation of PET oligomers. Structural and computational analysis reveal how a point mutation stabilizes the active site and reshapes the binding cleft, expading substrate scope.
Panagiota Karampa, Konstantinos Makryniotis, Theofani‐Iosifina Sousani, Evangelos Topakas, Vangelis Daskalakis, Maria Dimarogona   +5 more
wiley   +1 more source

Valosin‐containing protein counteracts ATP‐driven dissolution of FUS condensates through its ATPase activity in vitro

FEBS Letters, EarlyView.
Biomolecular condensates formed by fused in sarcoma (FUS) are dissolved by high ATP concentrations yet persist in cells. Using a reconstituted system, we demonstrate that valosin‐containing protein (VCP), an AAA+ ATPase, counteracts ATP‐driven dissolution of FUS condensates through its D2 ATPase activity.
Hitomi Kimura, Shin‐ichi Tate, Kyota Yasuda   +2 more
wiley   +1 more source

Çelik Gömmeli Kompozit Kolonların Sonlu Elemanlar Analizi ile Sayısal Karşılaştırılması

Düzce Üniversitesi Bilim ve Teknoloji Dergisi, 2021
Bu çalışma, çelik gömmeli kompozit kolonların, beton dayanımı ile boyuna donatı oranının değişimine göre eksenel basınç kuvveti etkisindeki davranışını açıklamaktadır.
Fethullah Uslu, Kıvanç Taşkın
doaj   +1 more source

Degradation mechanism of the von Willebrand factor A2 domain by nattokinase

FEBS Letters, EarlyView.
Nattokinase, a natto‐derived protease, exhibits potent antithrombotic effects. This study demonstrates that nattokinase directly cleaves the von Willebrand factor (vWF) A2 domain in vitro. Unlike the native regulator ADAMTS13, nattokinase degrades folded vWF independently of shear stress.
Ryuichi Hyakumoto, Masatomo So, Ayako Furukawa, Kenji Sugase   +3 more
wiley   +1 more source

Modulation of Homer1 EVH1 domain internal dynamics by putative autism‐associated mutations

FEBS Letters, EarlyView.
The putative autism‐associated M65I and S97L variants of the EVH1 domain of the postsynaptic scaffold protein Homer1 do not exhibit substantial changes in their overall structure or partner binding. Both of them, but especially the M65I variant, show altered internal dynamics relative to the wild‐type domain on the μs‐ms timescale, indicated by the ...
Fanni Farkas, Brigitta Maruzs, Zsófia E. Kálmán, Tomas Klumpler, Gyula Batta, Bálint Péterfia, Zoltán Gáspári   +6 more
wiley   +1 more source

Investigation into the towing performance of single-column composite bucket foundations

Shuili Shuiyun Gongcheng Xuebao
The single-column composite bucket foundation is a promising solution that combines the benefits of single-column and bucket foundations. It offers advantages such as reduced construction time and cost, as well as environmental friendliness. Towing plays
ZHANG Puyang, WANG Yiting, XIAO Jiandong, GAO Yang   +3 more
doaj   +1 more source

Salmonella lipopolysaccharide‐containing supported lipid bilayers as platforms to study bacteriophage interactions

FEBS Letters, EarlyView.
We present robust protocols for the preparation of supported lipid bilayers (SLBs) incorporating either Salmonella smooth LPS or outer membrane vesicles (OMVs). We use a combination of quartz crystal microbalance with dissipation (QCM‐D) and fluorescence microscopy to both characterize the SLBs of various compositions and to probe their interactions ...
Hudson P. Pace, Alyssa Borges, Charles Arnaud, Tove Karlsson, Nina K. Bröker, Stefanie Barbirz, Marta Bally   +6 more
wiley   +1 more source

Research on the Theoretical Models of FRP-Confined Gangue Aggregate Concrete Partially Filled Steel Tube Columns

Buildings
FRP-confined gangue aggregate concrete partially filled steel tubes (CGCPFTs) can not only effectively enhance the performance of coal gangue concrete, but also fully exploit the elastic-plastic mechanical behavior of the steel tubes.
Jian Wang, Junwu Xia, Chuanzhi Sun, Jinsheng Cheng, Shengbo Zhou, Jibing Deng   +5 more
doaj   +1 more source