Results 91 to 100 of about 279,015 (269)

Spatiotemporal and quantitative analyses of phosphoinositides – fluorescent probe—and mass spectrometry‐based approaches

FEBS Letters, EarlyView.
Fluorescent probes allow dynamic visualization of phosphoinositides in living cells (left), whereas mass spectrometry provides high‐sensitivity, isomer‐resolved quantitation (right). Their synergistic use captures complementary aspects of lipid signaling. This review illustrates how these approaches reveal the spatiotemporal regulation and quantitative
Hiroaki Kajiho, Shin Morioka, Junko Sasaki, Takehiko Sasaki   +3 more
wiley   +1 more source

Phosphatidylinositol 4‐kinase as a target of pathogens—friend or foe?

FEBS Letters, EarlyView.
This graphical summary illustrates the roles of phosphatidylinositol 4‐kinases (PI4Ks). PI4Ks regulate key cellular processes and can be hijacked by pathogens, such as viruses, bacteria and parasites, to support their intracellular replication. Their dual role as essential host enzymes and pathogen cofactors makes them promising drug targets.
Ana C. Mendes, Guilherme M. Azevedo, Amanda P. Barcellos, Diana Bahia   +3 more
wiley   +1 more source

Three-Dimensional Discrete Element Analysis of Bearing Characteristics of Concrete–Cored Sand–Gravel Pile Composite Foundation under Cyclic Dynamic Load

Buildings
Concrete-cored sand–gravel piles are a kind of composite pile formed by wrapping a concrete-cored pile with a sand–gravel shell, which has the advantages of both a rigid pile and bulk-material pile.
Binhui Ma, Wenbiao Tang, Tian Lan, Xu Deng, Bicheng Du   +4 more
doaj   +1 more source

Protein pyrophosphorylation by inositol pyrophosphates — detection, function, and regulation

FEBS Letters, EarlyView.
Protein pyrophosphorylation is an unusual signaling mechanism that was discovered two decades ago. It can be driven by inositol pyrophosphate messengers and influences various cellular processes. Herein, we summarize the research progress and challenges of this field, covering pathways found to be regulated by this posttranslational modification as ...
Sarah Lampe, Tanmay Kumar Mohanty, Rashna Bhandari, Dorothea Fiedler   +3 more
wiley   +1 more source

Settlement Calculation of Semi-Rigid Pile Composite Foundation on Ultra-Soft Soil under Embankment Load

Buildings
Ultra-soft soil is distributed in coastal areas around the world and has poor engineering properties. There is a significant difference in settlement between semi-rigid pile and surrounding soil under embankment load.
Fengxu Cao, Chaoliang Ye, Zhenxu Wu, Zitong Zhao, Hao Sun   +4 more
doaj   +1 more source

The planar cell polarity protein Vangl2 interacts with the PDZ‐domains of Scribble but not with a unique PDZ‐like domain in Inturned

FEBS Letters, EarlyView.
Structural and biochemical characterisations show that the planar cell polarity (PCP) protein Inturned harbours a unique PDZ‐like domain that does not bind canonical PDZ‐binding motifs (PBMs) like that of another PCP protein Vangl2. In contrast, the apical‐basal polarity protein Scribble contains four PDZ domains that bind Vangl2, but one PDZ domain ...
Stephan Wilmes, Jan Brysch, Carmen Gelze, Lilli Meier, Daniel Kümmel   +4 more
wiley   +1 more source

Calpain small subunit homodimerization is robust and calcium‐independent

FEBS Letters, EarlyView.
Calpains dimerize via penta‐EF‐hand (PEF) domains. Using single‐molecule force spectroscopy, we measured the strength and kinetics of PEF–PEF homodimer binding. The interaction is robust, shows a transient conformational step before dissociation, and remains largely insensitive to Ca2+.
Nesha May O. Andoy, Trina Dykstra‐MacPherson, Mathias A. Bell, Peter L. Davies, Ruby May A. Sullan   +4 more
wiley   +1 more source

Experimental study on mechanism and engineering characteristics of composite foundation with timber piles

Yantu gongcheng xuebao
As a traditional green low-carbon foundation reinforcement technology, timber piles have been widely used in soft foundation treatment of buildings in China and Abroad since ancient times.
SONG Yinkai 1, 2, 3, TONG Jianxing 1, 2, 3, ZHOU Shengbin 1, 2, 3, TANG Qiufeng 1, 2, 3, SUN Xunhai 1, 2, 3, LI Zhao 1, 2, 3
doaj   +1 more source

Structural insights into an engineered feruloyl esterase with improved MHET degrading properties

FEBS Letters, EarlyView.
A feruloyl esterase was engineered to mimic key features of MHETase, enhancing the degradation of PET oligomers. Structural and computational analysis reveal how a point mutation stabilizes the active site and reshapes the binding cleft, expading substrate scope.
Panagiota Karampa, Konstantinos Makryniotis, Theofani‐Iosifina Sousani, Evangelos Topakas, Vangelis Daskalakis, Maria Dimarogona   +5 more
wiley   +1 more source

Deformation mechanism and design method of embankment supported by composite foundation in soft soil regions

Yantu gongcheng xuebao
To clarify the settlement mechanisms of rigid-pile composite ground supporting embankments in soft soil regions during both construction and long-term service, and to propose an optimal pile arrangement strategy for effectively controlling pavement ...
WANG Hui 1, WANG Zhenyu 1, ZHENG Gang 2, WANG Haifeng 1, XU Zhengyi 2, ZHU Yujie 2, XIA Boyang 2
doaj   +1 more source