Results 81 to 90 of about 27,432 (257)

Recent trends and developments in pyrolysis-gas chromatography: review [PDF]

, 2008
Pyrolysis-gas chromatography (Py-GC) has become well established as a simple, quick and reliable analytical technique for a range of applications including the analysis of polymeric materials.
Arias, Armitage, Aslan, Asperger, Asperger, Asperger, Asperger, Bertini, Beyer, Blackledge, Blokker, Bonaduce, Bruck, Buckley, Buco, Campbell, Casas-Catalán, Causin, Challinor, Challinor, Challinor, Chang, Chiavari, Choi, Choi, Colombini, De Koeijer, De la Cruz-Canizares, Dignac, Doménech-Carbó, Doménech-Carbó, Dunn, Dzierzega-Lecznar, Dzierzega-Lecznar, Egan, Erickson, Evans, Evans, Fabbri, Fabbri, Fanter, Fifield, Flodin, Garcette-Lepecq, Gleixner, González-Vila, Goodacre, Greenwood, Greenwood, Greenwood, Greenwood, Guo, Gupta, Göbbels, Gözet, Halket, Halket, Heffenden, Herrera, Horrocks, Ikeya, Ishikawa, Islas, Jakab, Jamin, Jansson, Jarde, Jia, Jin, Jose Gonzalez-Rodriguez, Karina L. Sobeih, Kechang, Kochanowski, Kumooka, Lattimer, Lazaro, Learner, Levy, Li, Ling, Liu, Mao, Marinari, Marinari, Mark Baron, Marques, Maynard, Meruva, Metz, Moldoveanu, Mowry, Munson, Mäkinen, Nierop, Odermatt, Odermatt, Oguz, Ohshima, Osete-Cortina, Osete-Cortina, Osete-Cortina, Park, Parsi, Parsi, Parsi, Parsi, Partouche, Pasch, Peacock, Pel, Pel, Perng, Perng, Petsch, Poerschmann, Poirier, Prasad, Prati, Raghavan, Raulin, Ristau, Ristolainen, Rodriguez, Ross, Räisänen, Sakayanagi, Scalarone, Schmidt, Schwarzinger, Sephton, Sessions, Snyder, Stauffer, Stout, Stout, Stout, Suelves, Sugden, Sun, Sundarrajan, Suuronen, Takayasu, Takayasu, Tanczos, Thomsen, Tsai, Tsuge, Tsuge, Tzamtzis, Urakami, Uyar, Uyar, Vanderborgh, Volkman, Wampler, Wampler, Wampler, Wang, Wang, Wang, Wang, Wang, Wang, Wang, Wang, Wang, White, Wilkes, Wilkes, Yamamoto, Yang, Yaylayan, Yinon, Zhang, Zhou, Zhu, Zimmerman, Zlotnick, Çoban-Yildiz, Łakomy   +179 more
core   +1 more source

Promiscuous stimulation of HSP70 ATPase activity by parasite‐derived J‐domains

FEBS Open Bio, EarlyView.
The malaria parasite Plasmodium falciparum exports three highly homologous yet functionally divergent J‐domain proteins into human erythrocytes. Here, we show that J‐domains isolated from all three proteins effectively stimulate the ATPase activity of both endogenous host and exported parasite HSP70 chaperones.
Julian Barth, Moritz Koch, Le‐Han Rössner, Johanna Eichhorn, Denys Pogoryelov, Matthias P. Mayer, Jude M. Przyborski   +6 more
wiley   +1 more source

Seven Golden Rules for heuristic filtering of molecular formulas obtained by accurate mass spectrometry [PDF]

, 2007
Background Structure elucidation of unknown small molecules by mass spectrometry is a challenge despite advances in instrumentation. The first crucial step is to obtain correct elemental compositions.
Tobias Kind, Oliver Fiehn, C Djerassi, S Omura, V Wray, BG Buchanan, DL Olson, JC Lindon, ME Elyashberg, JM Halket, JF Zhang, T Kind, B Seebass, S Heuerding, MP Balogh, L Sleno, A Makarov, AJ Dempster, H Budzikiewicz, JR De Laeter, J Lederberg, AL Rockwood, O Fiehn, SE Stein, W Windig, DR Scott, N Huang, AA Pontet, MD Soffer, DA Laws, HE Dayringer, M Badertscher, S Noury, JK Senior, T Morikawa, J Braun, C Steinbeck, AW Jensen, DL Wheeler, T Fink, JL Faulon, DS Wishart, QY Wu, O Fiehn, RG Dromey, C Steinbeck, AM Richard, P Murray-Rust, WD Ihlenfeldt, SR Heller, SR Heller, J Chen, JJ Irwin, F Csizmadia   +53 more
core   +2 more sources

Development of human monoclonal antibodies against TARM1 by yeast display

FEBS Open Bio, EarlyView.
Human monoclonal antibodies against TARM1 are generated by yeast display‐guided selection. These antibodies bind to soluble and cell‐surface forms of TARM1. Also, these antibodies exhibit agonistic activity in the NFAT‐GFP reporter assay, indicating that TARM1 signaling can be functionally modulated by antibodies and suggesting TARM1 as a potential ...
Rikio Yabe, Mayumi Saeki, Masaaki Hashiguchi, Sayaka Ono, Kazuhisa Aoki, Hidetaka Tanno   +5 more
wiley   +1 more source

Analysing the significance of small conformational changes and low occupancy states in serial crystallographic data

FEBS Open Bio, EarlyView.
This protocol paper outlines methods to establish the success of a time‐resolved serial crystallographic experiment, by means of statistical analysis of timepoint data in reciprocal space and models in real space. We show how to amplify the signal from excited states to visualise structural changes in successful experiments.
Jake Hill, Yelyzaveta Pulnova, Elke De Zitter, Helen M. Ginn, Briony A. Yorke   +4 more
wiley   +1 more source

Screening and epitope characterization of Nidogen‐2‐specific nanobodies

FEBS Open Bio, EarlyView.
Camel immunization and phage display were employed to generate high‐affinity VHH nanobodies against Nidogen‐2. After library construction, biopanning, ELISA screening, sequencing, and recombinant expression, selected nanobodies were purified and characterized, leading to the preliminary exploration of a nanobody‐based sandwich ELISA for specific ...
Jianchuan Wen, Qianqian Cui, Zhongyun Lan, Yingjun Wang, Shuaiying Zhao, Wenxuan Feng, Yunfeng Liu, Qiting Huang, Dongna Zhang, Jianfeng Xu   +9 more
wiley   +1 more source

Digital twins to accelerate target identification and drug development for immune‐mediated disorders

FEBS Open Bio, EarlyView.
Digital twins integrate patient‐derived molecular and clinical data into personalised computational models that simulate disease mechanisms. They enable rapid identification and validation of therapeutic targets, prediction of drug responses, and prioritisation of candidate interventions.
Anna Niarakis, Philippe Moingeon
wiley   +1 more source

Evolutionarily divergent DUF4465 domains have a common vitamin B12‐binding function

FEBS Open Bio, EarlyView.
We show that DUF4465 family proteins, widespread across bacteria from gut microbiomes, hydrothermal vents, and soil, share a common vitamin B12‐binding function. These augmented β‐jellyroll proteins bind vitamin B12 via extended loops. Our findings establish sequence‐diverse DUF4465 proteins as a widespread class of B12‐binding proteins, highlighting ...
Charlea Clarke, Michal Banasik, Rokas Juodeikis, Martin J. Warren, Richard W. Pickersgill   +4 more
wiley   +1 more source

Systemic dysregulation of apolipoproteins in amyotrophic lateral sclerosis serum

FEBS Open Bio, EarlyView.
Amyotrophic lateral sclerosis (ALS) is a fatal disease that damages motor neurons. This study found that people with ALS show significant changes in blood fats and the proteins that carry them. Several apolipoproteins were higher, lipid balances were altered, and normal protein–lipid relationships were disrupted.
Finula I. Isik, Russell Pickford, Hannah C. Timmins, Olivier Piguet, Glenda M. Halliday, Matthew C. Kiernan, Woojin Scott Kim   +6 more
wiley   +1 more source

Large‐scale bidirectional arrayed genetic screens identify OXR1 and EMC4 as modifiers of αSynuclein aggregation

FEBS Open Bio, EarlyView.
Activation of the mitochondrial protein OXR1 increases pSyn129 αSynuclein aggregation by lowering ATP levels and altering mitochondrial membrane potential, particularly in response to MSA‐derived fibrils. In contrast, ablation of the ER protein EMC4 enhances autophagic flux and lysosomal clearance, broadly reducing α‐synuclein aggregates.
Sandesh Neupane, Lea Nikolić, Lorenzo Maraio, Thomas Goiran, Nathan Karpilovsky, Stefano Sellitto, Vangelis Bouris, Jiang‐An Yin, Ronald Melki, Edward A Fon, Adriano Aguzzi, Elena De Cecco   +11 more
wiley   +1 more source