Results 71 to 80 of about 295,466 (267)

Automated FRAP microscopy for high‐throughput analysis of protein dynamics in chromatin organization and transcription

FEBS Open Bio, EarlyView.
RoboMic is an automated confocal microscopy pipeline for high‐throughput functional imaging in living cells. Demonstrated with fluorescence recovery after photobleaching (FRAP), it integrates AI‐driven nuclear segmentation, ROI selection, bleaching, and analysis.
Selçuk Yavuz, Bart Geverts, Johan A. Slotman, Andrea Sacchetti, Stefan Prekovic, Martin E. van Royen, Adriaan B. Houtsmuller   +6 more
wiley   +1 more source

Computer Simulations of Charged Systems [PDF]

, 2001
In this brief contribution to the Proceedings of the NATO-ASI on ``Electrostatic Effects in Soft Matter and Biophysics'', which took place in Les Houches from Oct. 1-13, 2000, we summarize in short aspects of the simulations methods to study charged systems.
Holm, C., Kremer, K.
openaire   +3 more sources

Polarization‐resolved femtosecond Vis/IR spectroscopy tailored for resolving weak signals in biological samples using minimal sample volume

FEBS Open Bio, EarlyView.
Unique biological samples, such as site‐specific mutant proteins, are available only in limited quantities. Here, we present a polarization‐resolved transient infrared spectroscopy setup with referencing to improve signal‐to‐noise tailored towards tracing small signals. We provide an overview of characterizing the excitation conditions for polarization‐
Clark Zahn, Karsten Heyne
wiley   +1 more source

5G High Density Demand Dataset in Liverpool City Region, UK

Scientific Data
The wireless network data are a feasible way to understand the user behavior in a given environment and may be utilized for analysis, prediction and optimization. On the other hand, datasets from wireless service providers are not publicly available, and
Mukesh Kumar Maheshwari, Alessandro Raschellà, Michael Mackay, Max Hashem Eiza, Jon Wetherall, Jen Laing   +5 more
doaj   +1 more source

A light‐triggered Time‐Resolved X‐ray Solution Scattering (TR‐XSS) workflow with application to protein conformational dynamics

FEBS Open Bio, EarlyView.
Time‐resolved X‐ray solution scattering captures how proteins change shape in real time under near‐native conditions. This article presents a practical workflow for light‐triggered TR‐XSS experiments, from data collection to structural refinement. Using a calcium‐transporting membrane protein as an example, the approach can be broadly applied to study ...
Fatemeh Sabzian‐Molaei, Fredrik Orädd, Konstantinos Magkakis, Magnus Andersson   +3 more
wiley   +1 more source

Regional impacts of solar radiation modification on surface temperature and precipitation in Mainland Southeast Asia and the adjacent oceans

Scientific Reports
Solar radiation modification (SRM) has been proposed to temporarily reduce anthropogenic warming. This study presents an assessment of the regional impacts of SRM via solar dimming and stratospheric aerosol injection (SAI) on temperature and ...
Pornampai Narenpitak, Siriwat Kongkulsiri, Saifhon Tomkratoke, Sirod Sirisup   +3 more
doaj   +1 more source

Analysing the significance of small conformational changes and low occupancy states in serial crystallographic data

FEBS Open Bio, EarlyView.
This protocol paper outlines methods to establish the success of a time‐resolved serial crystallographic experiment, by means of statistical analysis of timepoint data in reciprocal space and models in real space. We show how to amplify the signal from excited states to visualise structural changes in successful experiments.
Jake Hill, Yelyzaveta Pulnova, Elke De Zitter, Helen M. Ginn, Briony A. Yorke   +4 more
wiley   +1 more source

Molecular dynamics simulations of positively selected codons in FcγRI reveal novel biochemical binding properties

FEBS Open Bio, EarlyView.
Evolutionary analysis across 32 placental mammals identified positive selection at residues H148 and W149 in the immune receptor FcγR1. Ancestral reconstruction combined with molecular dynamics simulations reveals how these mutations may influence receptor structure and dynamics, providing insight into the evolution of antibody recognition and immune ...
David A. Young, Omar Arias‐Gaguancela, Fiona Gibson, Morgan Grainger, Jodie Score, Amit Nathubhai, Mauricio Cafiero, Lee Richard Machado   +7 more
wiley   +1 more source

Analysis of the Effect of the Reliability of the NB-Iot Network on the Intelligent System

IEEE Access, 2019
With the rapid development of the Internet of Things (IoT) in recent years, the popularization of different segments of IoT has emerged. The IoT technology is slowly evolving toward intelligence, convenience, low power consumption, large connectivity ...
Gangyong Jia, Yujie Zhu, Youhuizi Li, Zongwei Zhu, Li Zhou   +4 more
doaj   +1 more source

THE ETHICS OF COMPUTER MODELING AND SIMULATION

2018 Winter Simulation Conference (WSC), 2018
Information and Communication ...
F. LeRon Shults, Wesley J. Wildman, Virginia Dignum   +2 more
openaire   +3 more sources