Results 61 to 70 of about 2,348,563 (303)
Degradation mechanism of the von Willebrand factor A2 domain by nattokinase
FEBS Letters, EarlyView.Nattokinase, a natto‐derived protease, exhibits potent antithrombotic effects. This study demonstrates that nattokinase directly cleaves the von Willebrand factor (vWF) A2 domain in vitro. Unlike the native regulator ADAMTS13, nattokinase degrades folded vWF independently of shear stress.
Ryuichi Hyakumoto +3 more
wiley +1 more source
4T1- and HC11-conditioned media contain extracellular vesicles.
, 2015 Conditioned culture media were absorbed onto carbon-coated grids, negatively stained with 1% uranyl acetate, and visualized by electron microscopy. The displayed images are representative images of (A) fresh medium, (B) 4T1-conditioned medium, (C) HC11 ...
Laurence Madera (104683) +3 more
core +1 more source
Beneficial Effect of Resveratrol on Conditioned Medium Human Adipose-derived Mesenchymal Stem Cell for Cutaneous Wound Healing Related Growth Factors [PDF]
Introduction: Cutaneous wound healing, a type of soft tissue injury, needs the interaction between multiple growth factors (epidermal growth factor [EGF], transforming growth factor [TGF], platelet-derived growth factor [PDGF], and vascular endothelial ...
Bagus Haryo Kusumaputra, - +10 more
core +1 more source
From mice to humans—divergent strategies for intestinal homeostasis and regeneration
FEBS Letters, EarlyView.Recent advances such as organoid genome editing, xenotransplantation, imaging, and whole‐genome sequencing have enabled direct studies of human intestinal stem cells (ISCs). These studies reveal species‐specific features, including slower ISC proliferation, distinct injury responses, slower somatic mutation accumulation in humans, and an inverse ...
Keiko Ishikawa +2 more
wiley +1 more source
Sterile B. thailandensis-conditioned medium kills flies.
, 2012 (A) Oregon-R males injected with sterile-filtered B. thailandensis-conditioned medium (CM) died after injection. Mock-infected (LB) and uninjected (NI) controls continued to live at least for the duration of this experiment; data is based on 3 ...
Martina Pilátová (117587) +1 more
core +1 more source
Design and analysis strategies for robust microbiome ageing research
FEBS Letters, EarlyView.The gut microbiome changes with age and associates with age‐related morbidity and mortality, establishing it as a potential biomarker and intervention target for ageing. Realising this potential requires methodological rigour, yet distinguishing biological signals from methodological artefacts remains challenging across cohorts. This review provides an
Mark Olenik +5 more
wiley +1 more source
FEBS Letters, EarlyView.CT10 regulator of kinase (CRK) and CRK‐Like (CRKL) are signaling adaptors driving cell adhesion, motility, differentiation, and proliferation. SH2‐domain containing (SH) proteins are enriched in YXXP motifs which when phosphorylated create preferred binding sites for CRK family SH2 domains.
Phoebe M. Cousens +8 more
wiley +1 more source
Inhibition of apoE proteolysis in presence of glia conditioned medium.
, 2014 A) rE3 and rE4 proteins were incubated with conditioned media obtained from primary cortical neurons (DIV22) for indicated times. Full length apoE and its proteolytic products were analyzed by western immunoblotting. Longer exposure of same blot is shown
Irfan Y. Tamboli (543932) +2 more
core +1 more source
Reconstructing enzyme evolution by protein engineering
FEBS Letters, EarlyView.Natural enzyme evolution can be retraced by protein engineering methods such as directed evolution, rational design, and ancestral sequence reconstruction. These approaches reveal how enzymes emerged from ligand‐binding scaffolds, developed varying substrate preferences, formed oligomeric complexes, adapted to environmental changes, and evolved novel ...
Lukas Drexler +2 more
wiley +1 more source
In vitro development of bovine embryos in Buffalo rat liver- or bovine oviduct-conditioned medium.
, 1994 A culture system for bovine embryos was developed using Buffalo rat liver cell (BRL) line-conditioned medium without serum. Zygotes, obtained by in vitro maturation and fertilization of oocytes, were cultured either in unconditioned medium (TCM 199 or ...
Dessy, Franz +4 more
core +1 more source