Results 31 to 40 of about 59,283 (218)
Single‐molecule DNA flow‐stretch assays for high‐throughput DNA–protein interaction studies
FEBS Open Bio, EarlyView.We describe an optimised single‐molecule DNA flow‐stretch assay that visualises DNA–protein interactions in real time. Linear DNA fragments are tethered to a surface and stretched by buffer flow for fluorescence imaging. Using λ and φX174 DNA, this protocol enhances reproducibility and accessibility, providing a versatile approach for studying diverse ...
Ayush Kumar Ganguli +8 more
wiley +1 more source
Engenharia Agrícola, 2009 Com os crescimentos da população e da demanda por alimentos, novas tecnologias têm surgido visando ao aumento da produtividade e à redução nos custos de produção. Se por um lado essas tecnologias elevam a produção e reduzem os custos, por outro o tráfego
Renato L. de Assis +3 more
doaj +1 more source
Instrumented Cone Penetrometer for Dense Layer Characterization
Sensors, 2020 Subsurface characterization is essential for a successful infrastructure design and construction. This paper demonstrates the use of an instrumented cone penetrometer (ICP) for a dense layer characterization at two sites.
Jong-Sub Lee, Yong-Hoon Byun
doaj +1 more source
Importin 7 mediates the nuclear import of HIV‐1 integrase via a specific interacting interface
FEBS Open Bio, EarlyView.HIV‐1 integrase enables viral DNA integration into the host genome. By binding to the core domain of the host protein Importin 7 via its C‐terminal domain, the integrase is transported across the nuclear membrane into the nucleus, where integration of the viral genome into host DNA takes place. This translocation is a critical step for subsequent viral
Juana Bana +5 more
wiley +1 more source
FEBS Open Bio, EarlyView.We describe detailed protocols for the purification and preparation of Marchantia polymorpha Auxin Response Factor 2 (MpARF2). This protein is fused to an MBP solubility tag and an mNG fluorescent tag and is purified from Escherichia coli. The presented procedures make it possible to study MpARF2 assemblies, which could arise from phase separation ...
Bas Janssen +5 more
wiley +1 more source
Dirac-like cone-based electromagnetic zero-index metamaterials
Light: Science & Applications, 2021 Metamaterials with a Dirac-like cone dispersion at the center of the Brillouin zone behave like an isotropic and impedance-matched zero refractive index material at the Dirac-point frequency.
Yang Li, C. T. Chan, Eric Mazur
doaj +1 more source
Protocol for quantifying miRNA trafficking across the endosomal membrane
FEBS Open Bio, EarlyView.An in vitro protocol measures miRNA uptake into endosomes isolated from mammalian cell extracts, which are free of subcellular contaminants. Performed at 37 °C in the presence of ATP, it ensures the import of single‐stranded miRNA into the endosomal lumen.
Syamantak Ghosh +2 more
wiley +1 more source
Engenharia Agrícola, 2004 Este trabalho apresenta duas metodologias para avaliar os níveis de resistência mecânica à penetração e auxiliar na tomada de decisão quanto à necessidade de se proceder à operação de subsolagem.
Gilmar da Silva +2 more
doaj +1 more source
Investigation of Adaptive Optics Imaging Biomarkers for Detecting Pathological Changes of the Cone Mosaic in Patients with Type 1 Diabetes Mellitus. [PDF]
PLoS ONE, 2016 PURPOSE:To investigate a set of adaptive optics (AO) imaging biomarkers for the assessment of changes of the cone mosaic spatial arrangement in patients with type 1 diabetes mellitus (DM1). METHODS:16 patients with ≥20/20 visual acuity and a diagnosis of
Marco Lombardo +5 more
doaj +1 more source
FEBS Open Bio, EarlyView.This minireview explores fluorination as a key strategy to optimize therapeutic molecules and nanocarriers. By incorporating different fluorinated motifs—from single atoms to linear and branched chains—drug delivery, cellular uptake, and colloidal stability can be improved and fine‐tuned.
Martina Beccalli +2 more
wiley +1 more source