Results 81 to 90 of about 132,626 (258)

Calpain small subunit homodimerization is robust and calcium‐independent

FEBS Letters, EarlyView.
Calpains dimerize via penta‐EF‐hand (PEF) domains. Using single‐molecule force spectroscopy, we measured the strength and kinetics of PEF–PEF homodimer binding. The interaction is robust, shows a transient conformational step before dissociation, and remains largely insensitive to Ca2+.
Nesha May O. Andoy, Trina Dykstra‐MacPherson, Mathias A. Bell, Peter L. Davies, Ruby May A. Sullan   +4 more
wiley   +1 more source

Structural insights into an engineered feruloyl esterase with improved MHET degrading properties

FEBS Letters, EarlyView.
A feruloyl esterase was engineered to mimic key features of MHETase, enhancing the degradation of PET oligomers. Structural and computational analysis reveal how a point mutation stabilizes the active site and reshapes the binding cleft, expading substrate scope.
Panagiota Karampa, Konstantinos Makryniotis, Theofani‐Iosifina Sousani, Evangelos Topakas, Vangelis Daskalakis, Maria Dimarogona   +5 more
wiley   +1 more source

A methionine‐lined active site governs carbocation stabilization and product specificity in a bacterial terpene synthase

FEBS Letters, EarlyView.
This study reveals a unique active site enriched in methionine residues and demonstrates that these residues play a critical role by stabilizing carbocation intermediates through novel sulfur–cation interactions. Structure‐guided mutagenesis further revealed variants with significantly altered product profiles, enhancing pseudopterosin formation. These
Marion Ringel, Carl P. O. Helmer, Shani Zev, Ronja Driller, Emily Buhr, Markus Reinbold, Renana Schwartz, Gabriel Foley, Mikael Boden, Daniel Garbe, Gerhard Schenk, Dan Thomas Major, Bernhard Loll, Thomas Brück   +13 more
wiley   +1 more source

Virial pressure in systems of active Brownian particles

, 2015
The pressure of suspensions of self-propelled objects is studied theoretically and by simulation of spherical active Brownian particles (ABP). We show that for certain geometries, the mechanical pressure as force/area of a confined systems can equally be
Gompper, Gerhard, Winkler, Roland G., Wysocki, Adam   +2 more
core   +2 more sources

Hyperosmotic stress induces PARP1‐mediated HPF1‐dependent mono(ADP‐ribosyl)ation

FEBS Letters, EarlyView.
Sorbitol‐induced hyperosmotic stress rapidly induces reversible mono(ADP‐ribosyl)ation (MARylation) on PARP1 without the signs of genotoxic signaling. We show that PARP1 autoMARylation is HPF1 dependent and forms hydroxylamine‐resistant O‐glycosidic linkages.
Anna Georgina Kopasz, Mihály Mérey, Rebeka Vásárhelyi, Ramóna Pék, Victor Imburchia, László Henn, Adrián Kószó, Nicholas D. Lakin, Ivan Ahel, Sébastien Huet, Ágnes Czibula, Gyula Timinszky   +11 more
wiley   +1 more source

Anisotropic Damage to Hard Brittle Shale with Stress and Hydration Coupling

Energies, 2018
Acoustic-wave velocities of shale rocks with different coring angles were tested by an acoustic-emission experiment under different confining pressures and soaking time of drilling fluid.
Junchuan Gui, Tianshou Ma, Ping Chen, Heyi Yuan, Zhaoxue Guo   +4 more
doaj   +1 more source

Leak detector [PDF]

, 1975
A detector for sensing a leaking of fluid pressures is reported. The detector is characterized by an hermetically sealed housing confining therewithin a first normalized pressure, a connector for coupling the housing in direct communication with a ...
Sawyer, J. T.
core   +1 more source

Organizing the interface—Plasma membrane architecture and receptor dynamics in virus‐cell interactions

FEBS Letters, EarlyView.
Plasma membranes contain dynamic nanoscale domains that organize lipids and receptors. Because viruses operate at similar scales, this architecture shapes early infection steps, including attachment, receptor engagement, and entry. Using influenza A virus and HIV‐1 as examples, we highlight how receptor nanoclusters, multivalent glycan interactions ...
Jan Schlegel, Christian Sieben
wiley   +1 more source

Experimental Study of Confining Pressure-Induced Fracture Network for Shale Gas Reservoir Under Triaxial Compression Conditions

Fractal and Fractional
The experimental study of shale fracture development is very important. As a channel of permeability, a fracture has a great influence on the development of shale gas.
Jinxuan Han, Ming Gao, Yubo Wu, Ali Raza, Pei He, Jianhui Li, Yanjun Lu, Manping Yang, Hongjian Zhu   +8 more
doaj   +1 more source

Creep Mechanics of the High-Stress Soft Rock under Grade Unloading

Advances in Civil Engineering, 2020
In order to study the creep behavior of deep soft rock, gritstone was chosen as the research subject, and a rock triaxial rheometer (Rock 600-50) and acoustic emission (AE) system (SH-II) were used to carry out the grade unloading confining pressure ...
Shunjie Huang, Xiangrui Meng, Guangming Zhao, Yingming Li, Gang Liu, Chunliang Dong, Xiang Cheng, Wensong Xu, Chongyan Liu, Jun Zhou   +9 more
doaj   +1 more source