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Measurement in the Confocal Microscope
2003All measurements require that the microscope must be aligned as accurately as possible, and the gain (or PMT voltage) and black level must be set to avoid any overflow or underflow. Measuring surface profiles and relative depths is straightforward and can be carried out to a higher accuracy than the depth resolution of the microscopes, even though the ...
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Applied Optics, 1993
A confocal image slicer for use in high-resolution spectrography in astrophysics is presented. The deviceimproves the light transmission of a high-resolution spectrograph by an (unprecedented) order of magnitude. The production of a prototype is described, and the first astronomical results obtained with the Anglo-Australian Telescope are presented ...
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A confocal image slicer for use in high-resolution spectrography in astrophysics is presented. The deviceimproves the light transmission of a high-resolution spectrograph by an (unprecedented) order of magnitude. The production of a prototype is described, and the first astronomical results obtained with the Anglo-Australian Telescope are presented ...
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Confocal spectroscopy in microstructures
Biophysical Chemistry, 1997Confocal instrumentation makes it possible to carry out spectroscopic measurements with a very high signal-to-background ratio. The transit of a single fluorescent molecule through the focal point of the light can be monitored with this method. The particle transport can be observed in transparent microchannels.
Brinkmeier, M. +3 more
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Overview of Confocal Microscopy
2009Born out of the need to overcome an imaging problem in the 1950s, confocal microscopes today allow researchers to go beyond simple imaging and ask biochemical questions. This chapter provides background information on the development of modern confocal microscopes, their uses and applications.
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Reflectance Confocal Microscopy
2015The ultimate goal of early melanoma diagnosis is the detection of all melanomas, while minimizing the number of excisions of nevi. To achieve this goal requires the clinician to not only be able to identify specific melanoma features but to also recognize the distinct aspects of nevi. To complicate the matter, several pigmented and nonpigmented lesions
LONGO, Caterina, PELLACANI, Giovanni
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Current Protocols in Cytometry, 2005
AbstractThis unit re‐examines confocal microscopy from a current perspective. It outlines many of the most modern applications of confocal microscopy and the issues surrounding them. The expanding applications of confocal microscopy demand both minor and major modifications of the technology to enhance imaging capabilities for a growing variety of ...
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AbstractThis unit re‐examines confocal microscopy from a current perspective. It outlines many of the most modern applications of confocal microscopy and the issues surrounding them. The expanding applications of confocal microscopy demand both minor and major modifications of the technology to enhance imaging capabilities for a growing variety of ...
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Artificial confocal microscopy for deep label-free imaging
Nature Photonics, 2023Xi Chen, Shenghua He, Chenfei Hu
exaly
2011
Imaging confocal microscopy is a well-known technology for the 3D measurement of surface topography. A confocal microscope is used for the acquisition of a sequence of confocal images through the depth of focus of the objective. The highest signal within the images of the sequence for each pixel correlates with the height position of the topography ...
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Imaging confocal microscopy is a well-known technology for the 3D measurement of surface topography. A confocal microscope is used for the acquisition of a sequence of confocal images through the depth of focus of the objective. The highest signal within the images of the sequence for each pixel correlates with the height position of the topography ...
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Tutorial: guidance for quantitative confocal microscopy
Nature Protocols, 2020James Jonkman +2 more
exaly