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Probing Chirality of a Lipid Tubular by Confocal Raman Microscopy
Journal of Nanoscience and Nanotechnology, 2010The chiral phospholipids 1,2-bis-(10,12-tricosadiynoyl)-sn-glycero-3-phosphocholine (DC8,9 PC) can self assemble into lipid nanotubules. This hollow cylindrical supramolecular structure shows promise in a number of biotechnological applications. The mechanism of lipid tubule formation was initiated by assembling of lipid bilayer sheets from amphiphilic
Jarinee, Kiang-ia +8 more
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Confocal Raman microscopy: common errors and artefacts
The Analyst, 2010Confocal Raman microscopy is a powerful tool for research and analysis in the chemical, materials and life sciences, particularly for non-destructive depth profiling of transparent systems. Unfortunately, many Raman microscopes are not optimally configured for this purpose, and so yield unnecessarily low signal-to-noise spectra with poor spatial ...
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New Twists and Turns for Confocal Raman Microscopy
Frontiers in Optics, 2006Confocal Raman microscopes usually reject elastically scattered light. This light, however, carries information about the target’s angular scattering properties. Imaging this rejected beam creates a multimodal microscope with chemical (Raman) and structural (elastic scatter) capabilities.
Andrew J. Berger, Zachary J. Smith
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Materials analysis using confocal Raman microscopy
Macromolecular Symposia, 1999AbstractThe recent development of Raman microscopes with high optical throughput and very sensitive CCD cameras has led to Raman spectroscopy again competing effectively with FTIR methods for materials analysis. Modern Raman instruments, designed to operate confocally without serious alignment or energy trade‐off problems, allow depth profiling of ...
Chris Sammon +4 more
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Raman Instrumentation for Confocal Raman Microscopy
2010Raman spectroscopy is a weak scattering technique in which typically less than one in a million excitation photons give rise to a single Raman photon. The situation is even more difficult in confocal Raman microscopy, as the detection volume is reduced by the small pinhole and thousands of spectra must be collected in a short period of time. Therefore,
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Confocal fluorescence and Raman microscopy in industrial research
Colloid & Polymer Science, 1998Modern chemical and pharmaceutical industrial research benefits from improved spectroscopic tools. New developments in confocal fluorescence and Raman microscopy lead to an increase in sensitivity, selectivity and speed of microscopic imaging and fluctuation analysis resulting in a better understanding of structure–property relationships essential for ...
W. Schrof +3 more
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Confocal Raman Microscopy of Streptococcus Sanguis and Mutans
Frontiers in Optics 2008/Laser Science XXIV/Plasmonics and Metamaterials/Optical Fabrication and Testing, 2008Confocal Raman microscopy has been used to differentiate the spectra from biofilms of oral bacterial species Streptococcus sanguis and mutans. Improvements in processing algorithms allow this study to be performed using glass as a substrate.
Brooke D. Beier +2 more
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[Technique of confocal Raman microscopy on erythrocytes].
Guang pu xue yu guang pu fen xi = Guang pu, 2010The technique of confocal Raman scanning microscopy (point scanning, line scanning and 2D scanning) and bright field imaging of living erythrocytes was investigated as a function of different scanning conditions at the excitation wavelength of 514 nm. The biological effect of the 514 nm laser radiation on the erythrocytes was also evaluated, so that a ...
Li-li, Kang, Yao-xiong, Huang, Man, Luo
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Imaging theory of nonlinear Raman confocal microscopy
Journal of Modern Optics, 2008A novel nonlinear Raman confocal microscopy utilizing Raman induced Kerr effect spectroscopy (RIKES) is presented in this paper. The imaging theory of RIKES confocal microscopy with Gaussian beam is derived. The imaging properties of RIKES confocal microscopy and the impact of different beam waist widths of Gaussian beam on the lateral and axial ...
L.N. Guo, Z.L. Tang, D. Xing
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Three-Dimensional Digital Confocal Raman Microscopy
Applied Spectroscopy, 1993We describe an iterative image restoration technique which functions as digital confocal microscopy for Raman images. We deconvolute the lateral and axial components of the microscope point spread function from a series of optical sections, to generate a stack of well-resolved Raman images which describe the three-dimensional topology of a sample.
Anurag Govil +2 more
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