Scientific Reports, 2017 Both function and dysfunction of serine protease inhibitors (serpins) involve massive conformational change in their tertiary structure but the dynamics facilitating these events remain poorly understood.
Michael Petersen +3 more
doaj +1 more source
A putative ATPase mediates RNA transcription and capping in a dsRNA virus
eLife, 2015 mRNA transcription in dsRNA viruses is a highly regulated process but the mechanism of this regulation is not known. Here, by nucleoside triphosphatase (NTPase) assay and comparisons of six high-resolution (2.9–3.1 Å) cryo-electron microscopy structures ...
Xuekui Yu +3 more
doaj +1 more source
Calmodulin: The switch button of calcium signaling
Tzu Chi Medical Journal, 2022 Calmodulin (CaM), a calcium sensor, decodes the critical calcium-dependent signals and converts them into the driving force to control various important cellular functions, such as ion transport.
Chiu-Fen Yang, Wen-Chin Tsai
doaj +1 more source
Conformational selection in protein binding and function
, 2014 Protein binding and function often involves conformational changes. Advanced NMR experiments indicate that these conformational changes can occur in the absence of ligand molecules (or with bound ligands), and that the ligands may 'select' protein ...
Paul, Fabian, Weikl, Thomas R.
core +1 more source
The Carboxyl-Terminal Segment of Apolipoprotein A-V Undergoes a Lipid-Induced Conformational Change [PDF]
, 2010 Apolipoprotein (apo) A-V is a 343-residue, multidomain protein that plays an important role in regulation of plasma triglyceride homeostasis. Primary sequence analysis revealed a unique tetraproline sequence (Pro293-Pro296) near the carboxyl terminus of ...
Lee, B. L. +4 more
core +2 more sources
Global conformational dynamics of a Y-family DNA polymerase during catalysis. [PDF]
PLoS Biology, 2009 Replicative DNA polymerases are stalled by damaged DNA while the newly discovered Y-family DNA polymerases are recruited to rescue these stalled replication forks, thereby enhancing cell survival.
Cuiling Xu +4 more
doaj +1 more source
Maximum Flux Transition Paths of Conformational Change
, 2010 Given two metastable states A and B of a biomolecular system, the problem is to calculate the likely paths of the transition from A to B. Such a calculation is more informative and more manageable if done for a reduced set of collective variables chosen ...
Berkowitz M. +40 more
core +1 more source
Kinetic Characterization and X-ray Structure of a Mutant of Haloalkane Dehalogenase with Higher Catalytic Activity and Modified Substrate Range [PDF]
, 1996 Conversion of halogenated aliphatics by haloalkane dehalogenase proceeds via the formation of a covalent alkyl-enzyme intermediate which is subsequently hydrolyzed by water.
Dijkstra, Bauke W., +7 more
core +3 more sources
High temperature sensitivity is intrinsic to voltage-gated potassium channels
eLife, 2014 Temperature-sensitive transient receptor potential (TRP) ion channels are members of the large tetrameric cation channels superfamily but are considered to be uniquely sensitive to heat, which has been presumed to be due to the existence of an ...
Fan Yang, Jie Zheng
doaj +1 more source
A conformational switch high-throughput screening assay and allosteric inhibition of the flavivirus NS2B-NS3 protease. [PDF]
PLoS Pathogens, 2017 The flavivirus genome encodes a single polyprotein precursor requiring multiple cleavages by host and viral proteases in order to produce the individual proteins that constitute an infectious virion.
Matthew Brecher +9 more
doaj +1 more source