Results 61 to 70 of about 497,883 (326)

Use of a distant reporter group as evidence for a conformational change in a sensory receptor [PDF]

, 1977
A highly sensitive method for demonstrating ligand-induced conformational changes in protein molecules in solution is described. The method utilizes an environmentally sensitive reporter group that is known to be distant from the active site.
Hartig, P. R., Koshland, D. E., Jr., Zukin, R. S.   +2 more
core  

Inherent flexibility and protein function: the open/closed conformational transition of the N-terminal domain of calmodulin

, 2008
The key to understanding a protein's function often lies in its conformational dynamics. We develop a coarse-grained variational model to investigate the interplay between structural transitions, conformational flexibility and function of N-terminal ...
Portman, John J., Tripathi, Swarnendu
core   +1 more source

Minimal Aggregate Size and Minimal Fusion Unit for the First Fusion Pore of Influenza Hemagglutinin-Mediated Membrane Fusion [PDF]

, 2000
The data of Melikyan et al. (J. Gen. Physiol. 106:783, 1995) for the time required for the first measurable step of fusion, the formation of the first flickering conductivity pore between influenza hemagglutinin (HA) expressing cells and planar bilayers,
Joe Bentz, Alford, Bentz, Bentz, Bentz, Bentz, Bentz, Bentz, Bentz, Blumenthal, Brandon, Brunner, Bullough, Carr, Carr, Chen, Chernomordik, Chernomordik, Chernomordik, Danieli, Dieckmann, Durell, Ellens, Epand, Gaudin, Godley, Gray, Gray, Hernandez, Körte, Körte, Kozlov, Markovic, Melikyan, Molski, Pak, Plonsky, Puri, Qiao, Razinkov, Razinkov, Ruigrok, Shangguan, Shangguan, Skehel, Skehel, Sosnick, Stegmann, Stegmann, Steinhauer, Torney, Tse, Weissenhorn, Wharton, White, Wilson, Yu, Zimmerberg   +57 more
core   +4 more sources

Structural insights into lacto‐N‐biose I recognition by a family 32 carbohydrate‐binding module from Bifidobacterium bifidum

FEBS Letters, EarlyView.
Bifidobacterium bifidum establishes symbiosis with infants by metabolizing lacto‐N‐biose I (LNB) from human milk oligosaccharides (HMOs). The extracellular multidomain enzyme LnbB drives this process, releasing LNB via its catalytic glycoside hydrolase family 20 (GH20) lacto‐N‐biosidase domain.
Xinzhe Zhang, Naoki Sunagawa, Toma Kashima, Kiyohiko Igarashi, Akimasa Miyanaga, Shinya Fushinobu   +5 more
wiley   +1 more source

Allo-network drugs: Extension of the allosteric drug concept to protein-protein interaction and signaling networks [PDF]

, 2013
Allosteric drugs are usually more specific and have fewer side effects than orthosteric drugs targeting the same protein. Here, we overview the current knowledge on allosteric signal transmission from the network point of view, and show that most ...
Csermely, Péter, Nussinov, Ruth, Szilágyi, András   +2 more
core   +2 more sources

Peptide‐based ligand antagonists block a Vibrio cholerae adhesin

FEBS Letters, EarlyView.
The structure of a peptide‐binding domain of the Vibrio cholerae adhesin FrhA was solved by X‐ray crystallography, revealing how the inhibitory peptide AGYTD binds tightly at its Ca2+‐coordinated pocket. Structure‐guided design incorporating D‐amino acids enhanced binding affinity, providing a foundation for developing anti‐adhesion therapeutics ...
Mingyu Wang, Grace Du, Charity Yongo‐Luwawa, Angelina Lu, Brett Kinrade, Kim Munro, Karl E. Klose, William D. Lubell, Peter Davies, Shuaiqi Guo   +9 more
wiley   +1 more source

Conformational Changes of Calmodulin on Calcium and Peptide Binding Monitored by Film Bulk Acoustic Resonators

Biosensors, 2011
Film bulk acoustic resonators (FBAR) are mass sensitive, label-free biosensors that allow monitoring of the interaction between biomolecules. In this paper we use the FBAR to measure the binding of calcium and the CaMKII peptide to calmodulin.
Janos Vörös, Johannes Ottl, Martin Nirschl   +2 more
doaj   +1 more source

Anion transport inhibitor binding to band 3 in red blood cell membranes. [PDF]

, 1983
The inhibitor of anion exchange 4,4'-dibenzoamido-2,2'-disulfonic stilbene (DBDS) binds to band 3, the anion transport protein in human red cell ghost membranes, and undergoes a large increase in fluorescence intensity when bound to band 3.
Dix, JA, Solomon, AK, Verkman, AS
core  

Specific lid-base contacts in the 26s proteasome control the conformational switching required for substrate degradation. [PDF]

, 2019
The 26S proteasome is essential for proteostasis and the regulation of vital processes through ATP-dependent degradation of ubiquitinated substrates. To accomplish the multi-step degradation process, the proteasomes regulatory particle, consisting of lid
Aufderheide, Bard, Bard, Bard, Bashore, Beckwith, Dambacher, de la Peña, Ding, Dong, Eisele, Elsasser, Finley, Glickman, Glickman, Goldstein, Groll, Hershko, Kachroo, Lander, Lasker, Longtine, Matyskiela, Myers, Nemec, Peth, Prakash, Rubin, Smith, Sone, Tomko, Unverdorben, Verma, VerPlank, VerPlank, Wehmer, Wendler, Wenzel, Worden, Worden, Yao, Zhu, Śledź   +42 more
core   +1 more source

Interplay between circadian and other transcription factors—Implications for cycling transcriptome reprogramming

FEBS Letters, EarlyView.
This perspective highlights emerging insights into how the circadian transcription factor CLOCK:BMAL1 regulates chromatin architecture, cooperates with other transcription factors, and coordinates enhancer dynamics. We propose an updated framework for how circadian transcription factors operate within dynamic and multifactorial chromatin landscapes ...
Xinyu Y. Nie, Jerome S. Menet
wiley   +1 more source