Conjugation-based genetic manipulation of Fusobacterium animalis [PDF]
mBioFusobacterium is an oncobacterium associated with colorectal cancer (CRC) development. Despite that F. animalis (Fa) dominates the CRC niche in humans, studies on the pathogenesis of Fusobacterium have been limited by the paucity of genetically tractable
Duhyun Ko, Wendy S. Garrett
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Genetic basis of I-complex plasmid stability and conjugation
PLoS Genetics, 2023 Plasmids are major drivers of increasing antibiotic resistance, necessitating an urgent need to understand their biology. Here we describe a detailed dissection of the molecular components controlling the genetics of I-complex plasmids, a group of ...
Zheng Jie Lian +5 more
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High-throughput conjugation reveals strain specific recombination patterns enabling precise trait mapping in Escherichia coli. [PDF]
PLoS GeneticsGenetic exchange is a cornerstone of evolutionary biology and genomics, driving adaptation and enabling the identification of genetic determinants underlying phenotypic traits.
Thibault Corneloup +12 more
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Enhancing conjugation from E. coli to Streptomyces coelicolor by incorporating traJ into mobilizable plasmids [PDF]
Applied Microbiology and BiotechnologyStreptomyces species are prolific producers of bioactive natural products, yet their genetic manipulation remains constrained by inefficient DNA delivery methods in many strains.
Paula Valdés-Chiara +4 more
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Host genetics regulate bacterial plasmid conjugation differentially in avian ceca explant co-culture through secretory miRNA [PDF]
npj Antimicrobials and ResistanceThe rapid emergence of multidrug-resistant virulent bacteria poses a global health concern. It is enabled through the lateral transfer of plasmid-carrying antibiotic resistance and virulence genes between bacteria.
Caleb Skow +3 more
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Analysis of two pheromone-responsive conjugative multiresistance plasmids carrying the novel mobile optrA locus from Enterococcus faecalis [PDF]
Infection and Drug Resistance, 2019 Yanhong Shang,1,2,* Dexi Li,2,* Xinxin Shan,2 Stefan Schwarz,3 Su-Mei Zhang,2 Yu-Xia Chen,2 Wuqing Ouyang,1 Xiang-Dang Du21Department of Basic Veterinary Medicine, College of Veterinary Medicine, Northwest A&F University, Shaanxi 712100, People’
Shang Y +7 more
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A complex genetic switch involving overlapping divergent promoters and DNA looping regulates expression of conjugation genes of a gram-positive plasmid. [PDF]
PLoS Genetics, 2014 Plasmid conjugation plays a significant role in the dissemination of antibiotic resistance and pathogenicity determinants. Understanding how conjugation is regulated is important to gain insights into these features.
Gayetri Ramachandran +7 more
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Multiple Layered Control of the Conjugation Process of the Bacillus subtilis Plasmid pLS20
Frontiers in Molecular Biosciences, 2021 Bacterial conjugation is the main horizontal gene transfer route responsible for the spread of antibiotic resistance, virulence and toxin genes.
Wilfried J. J. Meijer +6 more
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GENETIC ASPECTS OF INDIRECT HYPERBILIRUBINAEMIA IN NEWBORNS
Slovenska pediatrija, 2022 Indirect hyperbilirubinaemia is a common finding in the neonatal period. There are numerous causes of neonatal indirect hyperbilirubinemia, from excessive bilirubin production, impaired hepatic bilirubin uptake or bilirubin conjugation and increased ...
Katja Jarc Georgiev, Manca Velkavrh
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Communications Biology, 2023 Bacterial conjugation is a major horizontal gene transfer mechanism. While the functions encoded by many conjugative plasmids have been intensively studied, the contribution of recipient chromosome-encoded genes remains largely unknown. Here, we analyzed
Nancy Allard +4 more
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