Conjugation-based genetic manipulation of Fusobacterium animalis [PDF]
mBioFusobacterium is an oncobacterium associated with colorectal cancer (CRC) development. Despite that F. animalis (Fa) dominates the CRC niche in humans, studies on the pathogenesis of Fusobacterium have been limited by the paucity of genetically tractable
Duhyun Ko, Wendy S. Garrett
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Genetic basis of I-complex plasmid stability and conjugation
PLoS Genetics, 2023 Plasmids are major drivers of increasing antibiotic resistance, necessitating an urgent need to understand their biology. Here we describe a detailed dissection of the molecular components controlling the genetics of I-complex plasmids, a group of ...
Zheng Jie Lian +5 more
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Enhancing conjugation from E. coli to Streptomyces coelicolor by incorporating traJ into mobilizable plasmids [PDF]
Applied Microbiology and BiotechnologyStreptomyces species are prolific producers of bioactive natural products, yet their genetic manipulation remains constrained by inefficient DNA delivery methods in many strains.
Paula Valdés-Chiara +4 more
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High-throughput conjugation reveals strain specific recombination patterns enabling precise trait mapping in Escherichia coli. [PDF]
PLoS GeneticsGenetic exchange is a cornerstone of evolutionary biology and genomics, driving adaptation and enabling the identification of genetic determinants underlying phenotypic traits.
Thibault Corneloup +12 more
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Host genetics regulate bacterial plasmid conjugation differentially in avian ceca explant co-culture through secretory miRNA [PDF]
npj Antimicrobials and ResistanceThe rapid emergence of multidrug-resistant virulent bacteria poses a global health concern. It is enabled through the lateral transfer of plasmid-carrying antibiotic resistance and virulence genes between bacteria.
Caleb Skow +3 more
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Analysis of two pheromone-responsive conjugative multiresistance plasmids carrying the novel mobile optrA locus from Enterococcus faecalis [PDF]
Infection and Drug Resistance, 2019 Yanhong Shang,1,2,* Dexi Li,2,* Xinxin Shan,2 Stefan Schwarz,3 Su-Mei Zhang,2 Yu-Xia Chen,2 Wuqing Ouyang,1 Xiang-Dang Du21Department of Basic Veterinary Medicine, College of Veterinary Medicine, Northwest A&F University, Shaanxi 712100, People’
Shang Y +7 more
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Dye‐Conjugated Spinach RNA by Genetic Alphabet Expansion
Chemistry – A European Journal, 2022 AbstractLight‐emitting systems using an RNA aptamer‐dye pair, such as Spinach RNA, are an attractive method for imaging and tracing RNA expression in vitro and in vivo. We present an alternative Spinach method by genetic alphabet expansion using an unnatural base pair system, in which a dye‐conjugated unnatural base substrate is site‐specifically ...
Kyung Hyun Lee +5 more
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A complex genetic switch involving overlapping divergent promoters and DNA looping regulates expression of conjugation genes of a gram-positive plasmid. [PDF]
PLoS Genetics, 2014 Plasmid conjugation plays a significant role in the dissemination of antibiotic resistance and pathogenicity determinants. Understanding how conjugation is regulated is important to gain insights into these features.
Gayetri Ramachandran +7 more
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Conjugation-mediated genetic exchange in Legionella pneumophila [PDF]
Journal of Bacteriology, 1985 Genetic exchange mechanisms, to our knowledge, have not been reported for Legionella pneumophila, and consequently, studies on the genetic organization of L. pneumophila have not appeared in the literature. Here, we describe gene transfer mediated by broad host range conjugative plasmids in Legionella spp. Escherichia coli strains carrying plasmids RP1
L A, Dreyfus, B H, Iglewski
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Oligonucleotide-conjugated beads for transdominant genetic experiments [PDF]
Nucleic Acids Research, 2000 Transdominant genetics using expression libraries can identify proteins and peptides that affect cell division. In conjunction with these libraries, oligo-nucleotide-conjugated beads and flow cytometry were used to test a strategy that potentially expands the range of such genetic studies.
M J, Feldhaus +4 more
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