Results 111 to 120 of about 164,481 (302)

Gac two-component system in Pseudomonas syringae pv. tabaci is required for virulence but not for hypersensitive reaction [PDF]

open access: yes, 2008
Pseudomonas syringae pv. tabaci 6605 causes wildfire disease on host tobacco plants. To investigate the regulatory mechanism of the expression of virulence, Gac two-Component system-defective mutants, Delta gacA and Delta gacS, and a double mutant, Delta
A Bultreys   +38 more
core   +1 more source

An isoform of 14‐3‐3 protein regulates transbilayer lipid movement at the plasma membrane

open access: yesFEBS Letters, EarlyView.
Loss of 14‐3‐3ζ in CHO cells confers resistance to exogenous phosphatidylserine (PS) and impairs endocytosis‐independent inward flip‐flop of fluorescent PS at the plasma membrane. RNAi‐mediated knockdown reproduces this defect, while no additive effect is seen in ATP11C‐deficient cells.
Akiko Yamaji‐Hasegawa   +3 more
wiley   +1 more source

Surface Exclusion Revisited: Function Related to Differential Expression of the Surface Exclusion System of Bacillus subtilis Plasmid pLS20

open access: yesFrontiers in Microbiology, 2019
During conjugation a genetic element is transferred from a bacterial donor to a recipient cell via a connecting channel. It is the major route responsible for the spread of antibiotic resistance.
César Gago-Córdoba   +7 more
doaj   +1 more source

Role of HIV RNA structure in recombination and speciation: romping in purine A, keeps HTLV away

open access: yes, 2013
Extreme enrichment of the human immunodeficiency virus (HIV-1) RNA genome for the purine A parallels the mild purine-loading of the RNAs of most organisms.
Forsdyke, Donald R.
core   +1 more source

Conjugal Transfer of Genetic Material in Lactococci: A Review

open access: yesJournal of Dairy Science, 1989
Abstract The ability to mediate conjugal transfer of DNA is widespread among the lactococci. In the 9 yr since conjugation was first demonstrated in lactococci, a wide variety of phenotypes have been shown to be conjugally transferred. Recently, conjugation was used in constructing lactococcal strains with increased industrial utility.
James L. Steele, Larry L. McKay
openaire   +1 more source

The ubiquitin ligase RNF115 is required for the clearance of damaged lysosomes

open access: yesFEBS Letters, EarlyView.
Upon lysosomal rupture, an E3 ubiquitin ligase RNF115 translocates from the cytosol to the damaged lysosomal membrane. Moreover, RNF115 depletion impairs the clearance of damaged lysosomes, identifying it as a key regulator of lysosomal quality control.
Sae Nakanaga   +3 more
wiley   +1 more source

Establishment of a highly efficient conjugation protocol for Streptomyces kanamyceticus ATCC12853

open access: yesMicrobiologyOpen, 2019
Kanamycin B as the secondary metabolite of wild‐type Streptomyces kanamyceticus (S. kanamyceticus) ATCC12853 is often used for the synthesis of dibekacin and arbekacin.
Shuman Zhang   +6 more
doaj   +1 more source

Characterization and comparative analysis of antibiotic resistance plasmids isolated from a wastewater treatment plant.

open access: yesFrontiers in Microbiology, 2014
A wastewater treatment plant (WWTP) is an environment high in nutrient concentration with diverse bacterial populations and can provide an ideal environment for the proliferation of mobile elements such as plasmids.
Teddie O Rahube   +3 more
doaj   +1 more source

Yeasts [PDF]

open access: yes, 2018
Yeasts are a group of eukaryotic microfungi with a well-defined cell wall whose growth is either entirely unicellular or a combination of hyphal and unicellular reproduction. The approximately 1500 known yeast species belong to two distinct fungal phyla,
Lachance, Marc-Andre, Walker, Graeme M.
core   +2 more sources

In vitro models of cancer‐associated fibroblast heterogeneity uncover subtype‐specific effects of CRISPR perturbations

open access: yesMolecular Oncology, EarlyView.
Development of therapies targeting cancer‐associated fibroblasts (CAFs) necessitates preclinical model systems that faithfully represent CAF–tumor biology. We established an in vitro coculture system of patient‐derived pancreatic CAFs and tumor cell lines and demonstrated its recapitulation of primary CAF–tumor biology with single‐cell transcriptomics ...
Elysia Saputra   +10 more
wiley   +1 more source

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