Results 11 to 20 of about 163,659 (277)

Dye‐Conjugated Spinach RNA by Genetic Alphabet Expansion

Chemistry – A European Journal, 2022
AbstractLight‐emitting systems using an RNA aptamer‐dye pair, such as Spinach RNA, are an attractive method for imaging and tracing RNA expression in vitro and in vivo. We present an alternative Spinach method by genetic alphabet expansion using an unnatural base pair system, in which a dye‐conjugated unnatural base substrate is site‐specifically ...
Kyung Hyun Lee, Michiko Kimoto, Gota Kawai, Itaru Okamoto, Andrea Fin, Ichiro Hirao   +5 more
openaire   +3 more sources

Conjugation-mediated genetic exchange in Legionella pneumophila [PDF]

Journal of Bacteriology, 1985
Genetic exchange mechanisms, to our knowledge, have not been reported for Legionella pneumophila, and consequently, studies on the genetic organization of L. pneumophila have not appeared in the literature. Here, we describe gene transfer mediated by broad host range conjugative plasmids in Legionella spp. Escherichia coli strains carrying plasmids RP1
L A, Dreyfus, B H, Iglewski
openaire   +2 more sources

Oligonucleotide-conjugated beads for transdominant genetic experiments [PDF]

Nucleic Acids Research, 2000
Transdominant genetics using expression libraries can identify proteins and peptides that affect cell division. In conjunction with these libraries, oligo-nucleotide-conjugated beads and flow cytometry were used to test a strategy that potentially expands the range of such genetic studies.
M J, Feldhaus, M, Lualhati, K, Cardon, B, Roth, A, Kamb   +4 more
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Genetic organization of the bacterial conjugative transposon Tn916 [PDF]

Journal of Bacteriology, 1988
Tn916, which encodes resistance to tetracycline, is a 16.4-kilobase conjugative transposon originally identified on the chromosome of Streptococcus faecalis DS16. The transposon has been cloned in Escherichia coli on plasmid vectors, where it expresses tetracycline resistance; it can be reintroduced into S. faecalis via protoplast transformation.
E, Senghas, J M, Jones, M, Yamamoto, C, Gawron-Burke, D B, Clewell   +4 more
openaire   +2 more sources

Protein conjugation with genetically encoded unnatural amino acids [PDF]

Current Opinion in Chemical Biology, 2013
The site-specific incorporation of unnatural amino acids with orthogonal chemical reactivity into proteins enables the synthesis of structurally defined protein conjugates. Amino acids containing ketone, azide, alkyne, alkene, and tetrazine side chains can be genetically encoded in response to nonsense and frameshift codons.
Chan Hyuk, Kim, Jun Y, Axup, Peter G, Schultz   +2 more
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Genetic drift suppresses bacterial conjugation in spatially structured populations [PDF]

Biophysical Journal, 2014
ABSTRACTConjugation is the primary mechanism of horizontal gene transfer that spreads antibiotic resistance among bacteria. Although conjugation normally occurs in surface-associated growth (e.g., biofilms), it has been traditionally studied in well-mixed liquid cultures lacking spatial structure, which is known to affect many evolutionary and ...
Freese, PD, Korolev, KS, Jimenez, JI, Chen, IA   +3 more
openaire   +5 more sources

Genome-Wide Association Study Reveals Host Factors Affecting Conjugation in Escherichia coli

Microorganisms, 2022
The emergence and dissemination of antibiotic resistance threaten the treatment of common bacterial infections. Resistance genes are often encoded on conjugative elements, which can be horizontally transferred to diverse bacteria.
Laetitia Van Wonterghem, Matteo De Chiara, Gianni Liti, Jonas Warringer, Anne Farewell, Natalie Verstraeten, Jan Michiels   +6 more
doaj   +1 more source

A High-Throughput Method for Screening for Genes Controlling Bacterial Conjugation of Antibiotic Resistance

mSystems, 2020
The rapid horizontal transmission of antibiotic resistance genes on conjugative plasmids between bacterial host cells is a major cause of the accelerating antibiotic resistance crisis.
Hanna Alalam, Fabrice E. Graf, Martin Palm, Marie Abadikhah, Martin Zackrisson, Jonas Boström, Alfred Fransson, Chris Hadjineophytou, Linnéa Persson, Simon Stenberg, Matilda Mattsson, Payam Ghiaci, Per Sunnerhagen, Jonas Warringer, Anne Farewell   +14 more
doaj   +1 more source

Transfer of antibiotic resistance in Staphylococcus aureus [PDF]

, 2017
Staphylococcus aureus is a serious human pathogen with remarkable adaptive powers. Antibiotic-resistant clones rapidly emerge mainly by acquisition of antibiotic-resistance genes from other S. aureus strains or even from other genera.
Haaber, Jakob, Ingmer, Hanne, Penadés, José R.   +2 more
core   +1 more source

Ordering the bestiary of genetic elements transmissible by conjugation [PDF]

Mobile Genetic Elements, 2013
Phylogenetic reconstruction of three highly conserved proteins involved in bacterial conjugation (relaxase, coupling protein and a type IV secretion system ATPase) allowed the classification of transmissible elements in relaxase MOB families and mating pair formation MPF groups.
Maria Pilar Garcillán-Barcia, Fernando de la Cruz   +1 more
openaire   +2 more sources