Results 141 to 150 of about 69,612 (292)
“The Conquest of the Air” [PDF]
openaire +1 more source
Optimizing photoactivation of PA‐mCherry for optical pooled CRISPR screens
Photoactivatable PA‐mCherry finds widespread use to optically tag individual cells. However, confocal 405 nm UV laser‐scanning (normal scan) is much less efficient than widefield UV illumination, limiting the use of PA‐mCherry on confocal instruments. We remedy this limitation by reporting that rapid and repeated confocal scanning with a low‐intensity,
Sravasti Mukherjee +3 more
wiley +1 more source
Born in England, John Conquest followed the piper to Austin and launched his journal Music City Texas in 1989 to document the burgeoning live performance industry—especially the alt-country and singer-songwriter genres.
core
Strategy Selection and Outcomes of Tip-Detection in CTO-PCI: Intraplaque Tracking vs Antegrade Dissection and Re-Entry. [PDF]
Yamaji K +12 more
europepmc +1 more source
Versatile vector tools for efficient protein screening across multiple expression systems
A unified vector toolkit enables rapid protein expression screening across E. coli, insect, and mammalian cells. A single primer pair amplifies the target gene, which is inserted into any vector via a standardized interface. This streamlined workflow eliminates repeated cloning steps, accelerating the identification of optimal expression conditions for
Zhimin Zhu +5 more
wiley +1 more source
Enozertinib in <i>EGFR</i> Exon 20ins mutants: a game changer for CNS metastases, or a temporary conquest? [PDF]
Furuya N.
europepmc +1 more source
Directed evolution of enzymes at the crossroads of tradition and innovation
An iterative cycle of data‐driven enzyme optimization comprising four stages: genetic diversification of a template enzyme, expression of protein variants, high‐throughput evaluation, and machine‐learning‐guided redesign of the next variant library.
Maria Tomkova +2 more
wiley +1 more source
Intravascular Ultrasound-Guided "Move the Cap" Technique for Heavily Calcified Ostial Chronic Total Occlusion. [PDF]
Sudo K +9 more
europepmc +1 more source
TisIBP8, a fungal‐derived hyperactive ice‐binding protein, helps Caenorhabditis elegans survive dehydration. It localizes near cell membranes, reduces cell damage, and helps maintain membrane structure during drying. These results suggest that ice‐binding proteins can protect cells from dehydration stress as well as freezing stress.
Daiki Shimose +9 more
wiley +1 more source
Angiography- and IVUS-tip-detection-guided 3D wiring for CTO and non-CTO percutaneous coronary interventions. [PDF]
Okamura A +14 more
europepmc +1 more source

