Results 151 to 160 of about 1,527,554 (269)

Dual‐line Genome‐scale CRISPR Screening Enables Robust Target Gene Discovery

open access: yesAdvanced Science, EarlyView.
A species‐optimized CRISPR platform integrates efficient piggyBac delivery, genome‐scale sgRNA libraries, and parallel screening in two independently engineered Bactrocera dorsalis Cas9 cell lines. Cross‐line consensus analysis filters line‐specific effects, enriches candidates with reproducible in vivo phenotypes, and reveals conserved, species ...
Ziniu Li   +9 more
wiley   +1 more source

Chromosomal-level reference genome assembly of the North American wolverine (Gulo gulo luscus): a resource for conservation genomics. [PDF]

open access: yesG3 (Bethesda), 2022
Lok S   +9 more
europepmc   +1 more source

BOULE is Essential for the Dynamic Disassembly of Heat Shock Granules in Male Germ Cells

open access: yesAdvanced Science, EarlyView.
BOULE orchestrates stress granule disassembly in germ cells via a two‐pronged mechanism: it promotes G3BP1 ubiquitination by upregulating TRIM27, generating a signal for VCP/FAF2 recruitment, and it maintains G3BP1 and FAF2 protein levels. BOULE deficiency disrupts disassembly complex formation, leading to impaired heat shock granule clearance ...
Xin Li   +8 more
wiley   +1 more source

Saving the locals: a conservation genomics approach to the endangered Spanish Toothcarp, Aphanius iberus (Valenciennes, 1846). [PDF]

open access: yesSci Rep
Estarellas M   +13 more
europepmc   +1 more source

Conservation genomics of Agave tequilana Weber var. azul: low genetic differentiation and heterozygote excess in the tequila agave from Jalisco, Mexico. [PDF]

open access: yesPeerJ, 2022
Ruiz Mondragon KY   +9 more
europepmc   +1 more source

Asymmetric Evolution of Antennal Cell Types Underlies a Derived Ammonia‐Sensing Logic for Ecological Adaptation in Bactrocera dorsalis

open access: yesAdvanced Science, EarlyView.
Comparative antennal single‐nucleus transcriptomics reveals cell‐type‐dependent transcriptomic divergence in Bactrocera dorsalis, with similar structural cells but divergent sensory neurons. This neuronal diversification is associated with a distinct ammonia‐sensing pathway, linking female attraction to bird‐dropping‐associated cues with nutritional ...
Wei Liu   +7 more
wiley   +1 more source

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