Results 91 to 100 of about 10,462,770 (299)
FEBS Open Bio, EarlyView.Biophysical characterisation shows that NanX, a membrane transport protein from the major facilitator superfamily (MFS), forms both monomers and dimers after purification. AlphaFold modelling and substrate docking provide information on residues likely involved in substrate recognition for NanX and another MFS member, NanT.
Michael C. Newton‐Vesty +13 more
wiley +1 more source
FEBS Open Bio, EarlyView.We describe detailed protocols for the purification and preparation of Marchantia polymorpha Auxin Response Factor 2 (MpARF2). This protein is fused to an MBP solubility tag and an mNG fluorescent tag and is purified from Escherichia coli. The presented procedures make it possible to study MpARF2 assemblies, which could arise from phase separation ...
Bas Janssen +5 more
wiley +1 more source
Spray and Combustion Characteristics of CTL-Diesel Fuel Blends in a Constant-Volume Combustion Chamber. [PDF]
ACS Omega, 2023 Liu S +5 more
europepmc +1 more source
FEBS Open Bio, EarlyView.In this study, a novel esterase from the thermoacidophilic archaeon Thermoplasma acidophilum was biochemically and structurally characterized. Our results demonstrate that Ta0887 is a highly thermostable esterase that preferentially hydrolyzes p‐nitrophenyl hexanoate and possesses an α‐helical cap domain that likely contributes to its substrate ...
Alejandro Delgado‐Rey +4 more
wiley +1 more source
Small Corrections to 1989 NIST Constant-Volume Gas Thermometry Data. [PDF]
Metrologia, 2023 Harvey AH.
europepmc +1 more source
Recent insights into the molecular mechanism of ubiquinol oxidation by cytochrome bc1
FEBS Open Bio, EarlyView.The review reflects on the mechanism of the catalytic reaction in cytochrome bc1: the electron bifurcation that involves the separation of two electrons derived from the quinol oxidation to opposite sides of the enzyme across the membrane. This review summarizes the long‐standing effort to understand the mechanism of quinol oxidation catalyzed by ...
Anna Wójcik‐Augustyn +2 more
wiley +1 more source
Relationship of nuclear genome size, cell volume and nuclei volume in endosperm of Sorghum bicolor
Acta Biologica Slovenica, 2015 Endosperm cells of Sorghum bicolor undergo several rounds of endoreplication during seed development, resulting in somatic endopolyploidy with cells containing 3 C to 96 C nuclei (1 C represents the amount of DNA in an unreplicated haploid genome ...
Aleš Kladnik
doaj +1 more source
Using fluorescence fluctuation spectroscopy to quantify molecular dynamics within cells
FEBS Open Bio, EarlyView.Microscopy techniques like fluorescence correlation spectroscopy (FCS) and number and brightness analysis (N&B) allow to quantify concentrations, diffusion rates, and oligomerization of fluorescently labeled molecules in the living cell. In this protocol the principles of how to set up these types of experiments are described including calibration ...
Mark A. Hink
wiley +1 more source
Indian Journal of Anaesthesia, 2008 Use of spinal anaesthesia is gradually increasing in ambulatory setting. With the continuing controversy over the use of lidocaine for spinal anaesthesia, other local anaesthetics are being explored to achieve reliable spinal anaesthesia with rapid ...
Veena R Shah +2 more
doaj
, 1990 La sottoscritta ha ideato e curato il volume ,facendolo precedere da una prefazione: "Une tradition qui se perpétue en se renouvelant". Verte sull'importanza della critica italiana contemporanea circa i principali rappresentanti del Gruppo di Coppet che ...
OMACINI L., A.A.V.V, Omacini, Lucia
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