Results 61 to 70 of about 40,811 (265)

Screening and epitope characterization of Nidogen‐2‐specific nanobodies

FEBS Open Bio, EarlyView.
Camel immunization and phage display were employed to generate high‐affinity VHH nanobodies against Nidogen‐2. After library construction, biopanning, ELISA screening, sequencing, and recombinant expression, selected nanobodies were purified and characterized, leading to the preliminary exploration of a nanobody‐based sandwich ELISA for specific ...
Jianchuan Wen, Qianqian Cui, Zhongyun Lan, Yingjun Wang, Shuaiying Zhao, Wenxuan Feng, Yunfeng Liu, Qiting Huang, Dongna Zhang, Jianfeng Xu   +9 more
wiley   +1 more source

Promoter Methylation of HIV Coreceptor-Related Genes CCR5 and CXCR4: Original Research

Viruses
The persistence of human immunodeficiency virus (HIV) within viral reservoirs poses significant challenges to eradication efforts. Epigenetic alterations, including DNA methylation, are potential factors influencing the latency and persistence of HIV ...
Anna Esman, Svetlana Salamaikina, Alina Kirichenko, Michael Vinokurov, Darya Fomina, Kirill Sikamov, Arina Syrkina, Anastasia Pokrovskaya, Vasily Akimkin   +8 more
doaj   +1 more source

Providing Laboratory Monitoring of Environmental Objects During the XXII Olympic Winter Games and XI Paralympic Winter Games in Sochi

Эпидемиология и вакцинопрофилактика, 2015
The organization of laboratory environmental monitoring in the pre-Olympic period and during the XXII Olympic winter games and XI Paralympic winter games 2014.
A. Yu. Popova, A. A. Gorsky, A. S. Gus'kov, G. E. Ivanov, L. V. Chikina, V. S. Stepanov, E. S. Pochtareva, O. I. Aksenova, L. I. Shcerbina, V. V. Parhomenko, O. A. Kulichenko, N. S. Komarova, E. P. Shevchenko, V. P. Klinduhov, T. V. Grechanaja, M. A. Potemkina, M. I. Balaeva, V. A. Birjukov, I. I. Bozhko, S. Ch. Tesheva, L. S. Vechernjaja, V. A. Egorov, Ju. G. Daragan, O. V. Tushina, A. N. Kulichenko, D. V. Efremenko, E. A. Manin, D. A. Kovalev, V. E. Eldinova, Yu. V. Yunicheva, E. A. Bojko   +30 more
doaj   +1 more source

Acute caffeine treatment protects the developing retina from ischemia‐induced cell death

FEBS Open Bio, EarlyView.
Caffeine reduces cell death in the developing retina under ischemia (OGD). This effect does not involve BDNF upregulation or antioxidant pathways (NRF2/VEGF). Neuroprotection occurs mainly through adenosine A2A receptor antagonism, decreasing glutamate release and excitotoxicity, highlighting caffeine's potential as an acute neuroprotective agent in ...
Amanda Alves Nascimento, Danniel Pereira‐Figueiredo, Gabriella Grossi de Lima Dias, Gabriel Ferreira dos Santos, Mariana Rodrigues Pereira, Rafael Brito, Karin C Calaza   +6 more
wiley   +1 more source

Versatile vector tools for efficient protein screening across multiple expression systems

FEBS Open Bio, EarlyView.
A unified vector toolkit enables rapid protein expression screening across E. coli, insect, and mammalian cells. A single primer pair amplifies the target gene, which is inserted into any vector via a standardized interface. This streamlined workflow eliminates repeated cloning steps, accelerating the identification of optimal expression conditions for
Zhimin Zhu, Yaqing Liu, Lei Qin, Feng Yu, Qisheng Wang, Yahui Liu   +5 more
wiley   +1 more source

Time‐restricted feeding prior to Mycobacterium tuberculosis infection reduces tissue CD4+ T cells with limited impact on bacterial clearance

FEBS Open Bio, EarlyView.
Time‐restricted feeding (TRF) in mice increased liver fatty acid oxidation and decreased fatty acid biosynthesis. These alterations persisted when TRF was discontinued and the host was infected with Mycobacterium tuberculosis. Pre‐exposure to TRF did not alter tissue (lung and spleen) mycobacterial burden but significantly reduced CD3+ T cells in lungs
Ashish Gupta, Nidhi Yadav, Subhasmita Das, R. Rajendra Kumar Reddy, Nupur Sharma, Amol Ratnakar Suryawanshi, Jaswinder Singh Maras, Ranjan Kumar Nanda   +7 more
wiley   +1 more source

Flow cytometry for the analysis of virusneytralizing activity of antirabies serum and immunoglobulin drug

Инфекция и иммунитет, 2019
Here, we discuss development of f low cytometry technique for determining level of rabies antibodies in immune sera and immunoglobulin preparations, which is based on measuring fluorescence level in Vero cell line added with a mix of serially diluted ...
S. V. Generalov, A. L. Kravtsov, V. A. Kozhevnikov, Yu. K. Gavrilova, E. G. Abramova, A. K. Nikiforov   +5 more
doaj   +1 more source

MagmaFlow: A desktop platform for artificial intelligence‐driven expression analysis

FEBS Open Bio, EarlyView.
MagmaFlow is a free, no‐code platform for gene expression analysis. It generates interactive volcano plots, links genes to literature, pathways, and diseases, prioritizes candidates using millions of publications, identifies affected biological processes, builds network diagrams, and exports publication‐ready figures and reports for macOS and Windows ...
Carlos E. Buss, Ao Li, Eduardo H. Gilglioni, Mayank Bansal, Sumeet Pal Singh, Latifa Bakiri, Alessandra K. Cardozo, Esteban N. Gurzov   +7 more
wiley   +1 more source

Risk Prediction Models for Recurrence After Curative Treatment of Early‐Stage or Locally Advanced Lung Cancer: A Systematic Review

Aging and Cancer, EarlyView.
This systematic review synthesizes prognostic models for survival and recurrence in resected non‐small cell lung cancer. While many models demonstrate moderate to good discrimination, few are externally validated and reporting quality is variable, limiting clinical applicability and highlighting the need for robust, transparent model development ...
Evangeline Samuel, S. Tissera, E. Paul, J. Zalcberg, R. G. Stirling   +4 more
wiley   +1 more source

NGS amplification panel for HBV (Hepadnaviridae: Orthohepadnavirus) sequencing

Вопросы вирусологии
Introduction. Hepatitis B virus (HBV) remains a pressing global public health concern. The clinical course of the disease, particularly its tendency towards chronicity and response to therapy, is significantly influenced by the HBV genotype and specific ...
Mikhail D. Chanyshev, Natalia V. Vlasenko, German V. Roev, Ivan A. Kotov, Albina G. Glushchenko, Vera V. Makashova, Kamil F. Khafizov, Vasily G. Akimkin   +7 more
doaj   +1 more source