Results 41 to 50 of about 1,239,341 (306)

Reconstructing enzyme evolution by protein engineering

FEBS Letters, EarlyView.
Natural enzyme evolution can be retraced by protein engineering methods such as directed evolution, rational design, and ancestral sequence reconstruction. These approaches reveal how enzymes emerged from ligand‐binding scaffolds, developed varying substrate preferences, formed oligomeric complexes, adapted to environmental changes, and evolved novel ...
Lukas Drexler, Markus R. Busch, Reinhard Sterner   +2 more
wiley   +1 more source

Identification of heavy metal pollutants and their sources in farmland: an integrated approach of risk assessment and X-ray fluorescence spectrometry

Scientific Reports, 2022
Investigation and assessment of farmland pollution require an efficient method to identify heavy metal (HM) pollutants and their sources. In this study, heavy metals (HMs) in farmland were determined efficiently using high-precision X-ray fluorescence ...
Xiaosong Tian, Qing Xie, Min Fan, Guanqun Chai, Guanghui Li   +4 more
doaj   +1 more source

Identification of a Shiga toxin A‐derived peptide internalized into Gb3 receptor‐bearing cells via interaction with the Shiga toxin B subunit

FEBS Letters, EarlyView.
The process of internalization of the Shiga toxin A subunit via formation of a complex with the Shiga toxin B subunit, which specifically binds to the Gb3 receptor. The peptide is designed to act as a carrier of drugs into cancer cells. Here, we explored the potential of peptides derived from the catalytic A subunit of Shiga toxin (STxA) to be drug ...
Giulia Opassi, João C. Encarnação, Valeria Napolitano, Grzegorz Dubin, Grzegorz Popowicz, Karl Andersson, U. Helena Danielson   +6 more
wiley   +1 more source

An epithelial GPR35 isoform supports tumor‐associated transcriptional and metabolic phenotypes

FEBS Letters, EarlyView.
GPR35 generates two functionally distinct isoforms with previously unresolved roles. GPR35‐short mediates immune‐cell chemotaxis, while GPR35‐long is enriched in colorectal cancer epithelium, where it supports increased metabolism, proliferation, and tumor‐associated transcriptional programs.
Jørgen D. Rønneberg, Martine Hjeltnes, Josephine Krieger, Joshua E. Elias, Maria Stensland, Kristian Holm, Xiaojun Jiang, Chuki Khangsar, Tuula A. Nyman, Arthur Kaser, Johannes R. Hov, Tom H. Karlsen, Nicole C. Kaneider, Frode L. Jahnsen, Georg Schneditz   +14 more
wiley   +1 more source

Wheat straw- and maize straw-derived biochar effects on the soil cadmium fractions and bioaccumulation in the wheat–maize rotation system

Frontiers in Environmental Science, 2022
Wheat–maize rotation is one of the most popular systems and successful intensification cropping systems in Northern China, while soils in some of this area are contaminated by cadmium (Cd). However, few studies have performed experiments on the reduction
Yu Dun, Yu Dun, Chao Wu, Chao Wu, Ming Zhou, Xia Tian, Xia Tian, Guoqing Wu, Guoqing Wu   +8 more
doaj   +1 more source

Peripheral lysosomes recruit PLEKHG3 to focal adhesions and restrain protrusion dynamics

FEBS Letters, EarlyView.
Proximity‐dependent labeling at the LAMTOR complex revealed the Rho GEF PLEKHG3 as a lysosome‐proximal protein directing the study toward the influence of lysosome positioning on actin dynamics and cell motility. We show that PLEKHG3 colocalizes with lysosomes at focal adhesion sites and observe that forced peripheral dispersion of lysosomes hinders ...
Rainer Ettelt, Ana‐Maria Sandru, Georg Vucak, Sebastian Didusch, Biljana Riemelmoser, Karin Ehrenreiter, Markus Hartl, Lukas A. Huber, Manuela Baccarini   +8 more
wiley   +1 more source

UVC-Mirror for effective pathogens inactivation in air ducts [PDF]

E3S Web of Conferences
Improving the air quality of indoor environments (IAQ) is of utmost importance to safeguard public health as people spend about 80–90% of their time indoor. Efficient Ultraviolet germicidal irradiation (UVGI) system represents a strategic and sustainable
Treccani Laura, Rovetta Daniele, Zanetti Gabriele, Gobbi Emanuela, Turina Massimo, Lombini Matteo, Cortecchia Fausto, Diolaiti Emiliano, Malaguti Giuseppe, Bianco Andrea, Pareschi Giovanni, Mongelluzzo Giuseppe, Lessio Luigi   +12 more
doaj   +1 more source

The Key Factors for the Fate and Transport of Petroleum Hydrocarbons in Soil With Related in/ex Situ Measurement Methods: An Overview

Frontiers in Environmental Science, 2021
Once petroleum hydrocarbons (PHs) are released into the soil, the interaction between PHs and soil media is dependent not only upon the soil properties but also on the characteristics of PHs.
Liang Wang, Liang Wang, Ying Cheng, Ying Cheng, Ravi Naidu, Ravi Naidu, Mark Bowman   +6 more
doaj   +1 more source

Golgi enzymes are retrieved from the plasma membrane to the trans‐Golgi network

FEBS Letters, EarlyView.
Golgi enzymes are traditionally considered resident proteins retained within the Golgi apparatus. Here, we demonstrate that a subset transiently reaches the cell surface and is subsequently retrieved to the trans‐Golgi network via retrograde transport. Using a nanobody‐based toolkit, we uncover a dynamic trafficking cycle of several Golgi enzymes.
Dominik P. Buser, Tina Junne
wiley   +1 more source

Aquifers Recharge and Contamination by Leaking Sewers - ARCLeakS

, 2023
The ARCLeakS (Aquifers Recharge and Contamination by Leaking Sewers) project aims to fill the existing theoretical and practical knowledge gap on the fate of contaminants originating from leaking sewers in the subsurface of urban environments.
Andrea D'Aniello
core