Results 121 to 130 of about 3,143,654 (302)

Mass spectrometry based identification of AMP‐O‐Tris generated by Thermococcus onnurineus Cas10

FEBS Open Bio, EarlyView.
Isolated Thermococcus onnurineus Cas10 generates the noncanonical ATP‐derived product AMP‐O‐Tris while in Tris‐containing buffer as identified via mass spectrometry, revealing relaxed nucleophile selectivity under isolated conditions. These findings suggest that multiprotein Csm complex assembly restricts Cas10 reactivity toward canonical cyclic ...
Su‐Jin Lee, Ga Seul Lee, JinHan Kim, Kwang‐Hyun Park, Seong‐Ryung Go, Jeong Hee Moon, Eui‐Jeon Woo   +6 more
wiley   +1 more source

Refinement of amino‐acid conformation vs. difference density maps in time‐resolved serial femtosecond crystallography data analysis

FEBS Open Bio, EarlyView.
The dFoCC pipeline starts with observed DED and resting‐state coordinates, which are then used to generate a library of triggered states. Correlation analysis of the calculated DED features of each candidate vs observed DED permits quantitative evaluation of candidate structural quality.
Meng Iao Fong, Yuhei Hosokawa, Lars Oliver Essen, Manuel Maestre‐Reyna   +3 more
wiley   +1 more source

Integrating natural history collections and comparative genomics to study the genetic architecture of convergent evolution

Philosophical Transactions of the Royal Society of London. Biological Sciences, 2019
Evolutionary convergence has been long considered primary evidence of adaptation driven by natural selection and provides opportunities to explore evolutionary repeatability and predictability.
Sangeet Lamichhaney, D. Card, P. Grayson, J. Tonini, Gustavo A. Bravo, Kathrin Näpflin, Flavia Termignoni-García, Christopher R. Torres, F. Burbrink, J. Clarke, Timothy B Sackton, S. Edwards   +11 more
semanticscholar   +1 more source

Directed evolution of enzymes at the crossroads of tradition and innovation

FEBS Open Bio, EarlyView.
An iterative cycle of data‐driven enzyme optimization comprising four stages: genetic diversification of a template enzyme, expression of protein variants, high‐throughput evaluation, and machine‐learning‐guided redesign of the next variant library.
Maria Tomkova, Andrej Mirossay, Erik Sedlak   +2 more
wiley   +1 more source

Convergent Evolution of Mitochondrial Genes in Deep-Sea Fishes

Frontiers in Genetics, 2019
Deep seas have extremely harsh conditions including high hydrostatic pressure, total darkness, cold, and little food and oxygen. The adaptations of fishes to deep-sea environment apparently have occurred independently many times.
Xuejuan Shen, Zhiqing Pu, Xiao Chen, Robert W. Murphy, Yongyi Shen, Yongyi Shen, Yongyi Shen   +6 more
doaj   +1 more source

The Kalanchoë genome provides insights into convergent evolution and building blocks of crassulacean acid metabolism

Nature Communications, 2017
Crassulacean acid metabolism (CAM) is a water-use efficient adaptation of photosynthesis that has evolved independently many times in diverse lineages of flowering plants.
Xiaohan Yang, Xiaohan Yang, Rongbin Hu, Heng-fu Yin, J. Jenkins, S. Shu, Haibao Tang, Degao Liu, Deborah Weighill, Deborah Weighill, Won Yim, Jungmin Ha, K. Heyduk, D. Goodstein, Haobo Guo, Robert C. Moseley, Robert C. Moseley, Elisabeth Fitzek, S. Jawdy, Zhihao Zhang, Meng Xie, J. Hartwell, J. Grimwood, P. Abraham, Ritesh Mewalal, Juan D. Beltrán, Susanna F. Boxall, L. Dever, Kaitlin J. Palla, Kaitlin J. Palla, Rebecca L. Albion, Travis M. Garcia, Jesse A. Mayer, Sung Don Lim, C. M. Wai, P. Peluso, R. V. Buren, H. C. D. Paoli, H. C. D. Paoli, A. Borland, A. Borland, Hong Guo, Jin‐Gui Chen, W. Muchero, Yanbin Yin, Daniel Jacobson, Daniel Jacobson, T. Tschaplinski, R. Hettich, Ray Ming, Ray Ming, K. Winter, Jim Leebens-Mack, J. C. Smith, J. Cushman, J. Schmutz, G. Tuskan   +56 more
semanticscholar   +1 more source

Evolution and disease converge in the mitochondrion

Biochimica et Biophysica Acta (BBA) - Bioenergetics, 2010
Mitochondrial DNA (mtDNA) mutations are long known to cause diseases but also underlie tremendous population divergence in humans. It was assumed that the two types of mutations differ in one major trait: functionality. However, evidence from disease association studies, cell culture and animal models support the functionality of common mtDNA genetic ...
Mishmar, D., Zhidkov, I.
openaire   +2 more sources

How phagocytic cells kill bacteria: Lessons from a professional killer

FEBS Open Bio, EarlyView.
How phagocytic cells ingest and kill bacteria has been studied for more than a century, but many questions remain unanswered. The study of the amoeba Dictyostelium discoideum brings new answers, and new questions. Professional phagocytic cells such as neutrophils and macrophages, as well as free‐living soil amoebae like Dictyostelium discoideum, employ
Otmane Lamrabet, Pierre Cosson
wiley   +1 more source

MagmaFlow: A desktop platform for artificial intelligence‐driven expression analysis

FEBS Open Bio, EarlyView.
MagmaFlow is a free, no‐code platform for gene expression analysis. It generates interactive volcano plots, links genes to literature, pathways, and diseases, prioritizes candidates using millions of publications, identifies affected biological processes, builds network diagrams, and exports publication‐ready figures and reports for macOS and Windows ...
Carlos E. Buss, Ao Li, Eduardo H. Gilglioni, Mayank Bansal, Sumeet Pal Singh, Latifa Bakiri, Alessandra K. Cardozo, Esteban N. Gurzov   +7 more
wiley   +1 more source

Multiple convergent supergene evolution events in mating-type chromosomes

Nature Communications, 2018
Supergenes result from beneficial linkage and recombination suppression between two or more genes. Giraud and colleagues use whole genome sequencing data to show convergent evolution of supergenes on mating-type chromosomes in multiple closely-related ...
Sara Branco, Fantin Carpentier, Ricardo C. Rodríguez de la Vega, Hélène Badouin, Alodie Snirc, Stéphanie Le Prieur, Marco A. Coelho, Damien M. de Vienne, Fanny E. Hartmann, Dominik Begerow, Michael E. Hood, Tatiana Giraud   +11 more
doaj   +1 more source