Results 61 to 70 of about 592,843 (298)
We report that some menthol‐like cooling compounds, including (R)‐(‐)‐carvone, act as inhibitors of TAS2R31 and TAS2R43, which are taste receptors responsible for the intrinsic bitter aftertastes of saccharin and acesulfame K. However, there was little correlation between the intensity of the cooling sensation and the potency of bitterness inhibition ...
Miyuu Saito, Takumi Misaka
wiley +1 more source
The thermal conductivity of clustered nanocolloids
We quantify the effect of clustering on the thermal conductivity of colloidal dispersions using silane-treated silica, a system engineered to exhibit reversible clustering under well-controlled conditions.
Saba Lotfizadeh+2 more
doaj +1 more source
Structural and functional characterization of chitinase from carnivorous plant Drosera adelae
A class I chitinase from the carnivorous plant Drosera adelae was expressed and purified using a yeast system, revealing high enzymatic activity. Structural analyses of the catalytic and chitin‐binding domains identified key tyrosine residues involved in substrate binding, offering insights into the enzyme's adaptation for insect digestion.
Kazunari Yoneda+7 more
wiley +1 more source
Tryptophan metabolite atlas uncovers organ, age, and sex‐specific variations
Tryptophan metabolites were analyzed across twelve organs, the central nervous system, and serum in male and female mice at three life stages. We found tissue‐, sex‐, and age‐specific differences, including increased indole‐3‐pyruvate and kynurenine in aging males.
Lizbeth Perez‐Castro+8 more
wiley +1 more source
Soil copper (Cu) pollution is a serious environmental risk in the Panax notoginseng planting area. However, the effect of Cu on soil microbial metabolism and nutrient cycling in this area remains unknown.
Tong Wang+11 more
doaj +1 more source
Cloning, expression, purification, and structural modeling of the Chandipura virus matrix protein
This research protocol offers a guide for the cloning, expression, and purification of the Chandipura virus matrix protein using E. coli. It also includes a step‐by‐step procedure for cloning, expressing, and conducting fluorescence imaging of GFP‐fused Chandipura virus matrix protein in mammalian cell lines.
Mariana Grieben
wiley +1 more source
Reducing inter-unit pollutant transfer through multifamily unit compartmentalization
Compartmentalization, which refers to air sealing each unit within a building from the exterior and other interior spaces, has been proposed as a strategy to improve indoor air quality and energy efficiency in multifamily buildings.
Scott W. Adler+2 more
doaj +1 more source
Cool pavements designate alternative pavements designed to reduce their contribution to urban heating. Urban heating generally refers to the sensible heat exchanged with the atmosphere by urban materials but can also include the radiative load they impose on pedestrians.
openaire +3 more sources
Homologous expression and purification of human HAX‐1 for structural studies
This research protocol provides detailed instructions for cloning, expressing, and purifying large quantities of the intrinsically disordered human HAX‐1 protein, N‐terminally fused to a cleavable superfolder GFP, from mammalian cells. HAX‐1 is predicted to undergo posttranslational modifications and to interact with membranes, various cellular ...
Mariana Grieben
wiley +1 more source
Thrombolytic proteins profiling: High‐throughput activity, selectivity, and resistance assays
We present optimized biochemical protocols for evaluating thrombolytic proteins, enabling rapid and robust screening of enzymatic activity, inhibition resistance, and fibrin affinity, stimulation, and selectivity. The outcome translates to key clinical indicators such as biological half‐life and bleeding risk. These assays streamline the development of
Martin Toul+3 more
wiley +1 more source