Results 61 to 70 of about 1,499,819 (348)

Homologous expression and purification of human HAX‐1 for structural studies

open access: yesFEBS Open Bio, EarlyView.
This research protocol provides detailed instructions for cloning, expressing, and purifying large quantities of the intrinsically disordered human HAX‐1 protein, N‐terminally fused to a cleavable superfolder GFP, from mammalian cells. HAX‐1 is predicted to undergo posttranslational modifications and to interact with membranes, various cellular ...
Mariana Grieben
wiley   +1 more source

A New Alternate Method to Reuse Rehashed Edible Oil for the Betterment of Society - Dual Benefit Approach in Photovoltaic Modules

open access: yesIEEE Access, 2021
The Photovoltaic (PV) cell converts a portion of incident irradiation of visible wavelength into electricity and the remaining into heat, thereby decreasing the electrical efficiency. Hence, it is necessary to transfer the heat generated in the module by
Prince Winston David   +8 more
doaj   +1 more source

Where Do Cooling Flows Cool? [PDF]

open access: yesThe Astrophysical Journal, 2000
18 pages with 6 figures; accepted by Astrophysical ...
Brighenti, Fabrizio, Mathews, William G.
openaire   +2 more sources

Thrombolytic proteins profiling: High‐throughput activity, selectivity, and resistance assays

open access: yesFEBS Open Bio, EarlyView.
We present optimized biochemical protocols for evaluating thrombolytic proteins, enabling rapid and robust screening of enzymatic activity, inhibition resistance, and fibrin affinity, stimulation, and selectivity. The outcome translates to key clinical indicators such as biological half‐life and bleeding risk. These assays streamline the development of
Martin Toul   +3 more
wiley   +1 more source

Radiative sky cooling: Fundamental principles, materials, and applications

open access: yesApplied Physics Reviews, 2019
Radiative sky cooling cools an object on the earth by emitting thermal infrared radiation to the cold universe through the atmospheric window (8–13 μm). It consumes no electricity and has great potential to be explored for cooling of buildings, vehicles,
Dongliang Zhao   +6 more
semanticscholar   +1 more source

SmallTalk: a novel small‐sized fusion tag for peptide expression and purification

open access: yesFEBS Open Bio, EarlyView.
The SmallTalk fusion tag allows for the efficient expression and purification of soluble recombinant proteins or peptides in Escherichia coli. Testing with SmallTalk‐GFP confirmed that the proteins were soluble and folded correctly, while SmallTalk‐Bin1b maintained its antimicrobial activity against various bacterial isolates. This streamlined workflow
Atika Tariq   +3 more
wiley   +1 more source

Electromagnetic thermal coupling analysis for a novel cooling system of an axial flux hub motor

open access: yesIET Electric Power Applications, 2022
In this paper, a novel cooling system of the yokeless and segmented armature axial flux machine (YASA) applied in‐wheel traction is presented.
Jiujian Chang, Chen Wang
doaj   +1 more source

The collapse of atomically cooled primordial haloes – I. High Lyman–Werner backgrounds [PDF]

open access: hybrid, 2023
Samuel Patrick   +3 more
openalex   +1 more source

FGFR Like1 drives esophageal cancer progression via EMT, PI3K/Akt, and notch signalling: insights from clinical data and next‐generation sequencing analysis

open access: yesFEBS Open Bio, EarlyView.
Clinical analysis reveals significant dysregulation of FGFRL1 in esophageal cancer (EC) patients. RNAi‐coupled next‐generation sequencing (NGS) and in vitro study reveal FGFRL1‐mediated EC progression via EMT, PI3K/Akt, and Notch pathways. Functional assays confirm its role in tumor growth, migration, and invasion.
Aprajita Srivastava   +3 more
wiley   +1 more source

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