Results 161 to 170 of about 9,621,488 (249)

Purification and preparation of Marchantia polymorpha Auxin Response Factor 2 for phase separation studies

FEBS Open Bio, EarlyView.
We describe detailed protocols for the purification and preparation of Marchantia polymorpha Auxin Response Factor 2 (MpARF2). This protein is fused to an MBP solubility tag and an mNG fluorescent tag and is purified from Escherichia coli. The presented procedures make it possible to study MpARF2 assemblies, which could arise from phase separation ...
Bas Janssen, Robin Romein, Willy A. M. van den Berg, Simon Lindhoud, Jan Willem Borst, Carlo P. M. van Mierlo   +5 more
wiley   +1 more source

Copy Number Variant Architecture of Child Psychopathology and Cognitive Development in the ABCD Study. [PDF]

Am J Psychiatry
Sha Z, Sun KY, Jung B, Barzilay R, Moore TM, Almasy L, Forsyth JK, Prem S, Gandal MJ, Seidlitz J, Glessner JT, Alexander-Bloch AF.   +11 more
europepmc   +1 more source

Copy number variant analysis for syndromic congenital heart disease in the Chinese population. [PDF]

Hum Genomics, 2022
Li P, Chen W, Li M, Zhao Z, Feng Z, Gao H, Suo M, Xu Z, Tian G, Wu F, Wei S, Huang G.   +11 more
europepmc   +1 more source

Avidin is evolutionarily conserved in fish but dispensable for development and resistance against Streptococcus agalactiae in zebrafish

FEBS Open Bio, EarlyView.
The presence of biotin‐binding avidin proteins in fish and their biological significance are poorly characterized. We cataloged fish avidins and demonstrate that they are widely present and evolutionarily conserved. We created avd knockout zebrafish and show that zebavidin is dispensable for development and that resistance of avd knockout embryos in ...
Anni K. Saralahti, Markus J. T. Ojanen, Mataleena Parikka, Otto Kauko, Mika Rämet, Vesa P. Hytönen   +5 more
wiley   +1 more source

Phenotypic spectrum of 11p15.5 duplications: parent-of-origin and copy number variant size shape outcomes from Silver-Russell to Beckwith-Wiedemann syndromes. [PDF]

Hum Genomics
Wang C, Zhang H, Wang M, Gao J, Lv Y, Huang J, Pan S, Wu D, Kang B, Hou Q, Liao S.   +10 more
europepmc   +1 more source

A Polynesian-specific copy number variant encompassing the MICA gene associates with gout. [PDF]

Hum Mol Genet, 2022
Wang K, Cadzow M, Bixley M, Leask MP, Merriman ME, Yang Q, Li Z, Takei R, Phipps-Green A, Major TJ, Topless R, Dalbeth N, King F, Murphy R, Stamp LK, de Zoysa J, Wang Z, Shi Y, Merriman TR.   +18 more
europepmc   +1 more source

GelMA‐based 3D spheroids recapitulate transcriptomic and functional hallmarks of myeloid sarcoma

FEBS Open Bio, EarlyView.
GelMA 5% hydrogels support the formation of myeloid leukemia spheroids that recapitulate MS‐specific features, including G1 arrest, apoptosis, and ECM‐driven transcriptomic reprogramming. The 3D model mimicked soft‐tissue‐like stiffness and oxygen conditions, and transcriptomic convergence with primary MS samples confirmed its utility as a preclinical ...
Nicolas Germain, Elise Buret, Le Hoang Thanh Nguyen, Marion Cocheteux, Nicolas Touya, Mélanie Dhayer, Romain Dubois, Frank Lafont, Sebastien Janel, Patrice Maboudou, Steve Lancel, Philippe Marchetti   +11 more
wiley   +1 more source

Protein S Levels to Guide Exon-Level Copy Number Variant Analysis of <i>PROS1</i> for Confirming Hereditary Protein S Deficiency: A 10-Year Single-Center Experience. [PDF]

Ann Lab Med
Yu S, Park MS, Kim HY, Park TK, Chang SA, Kim DK, Kim HJ.   +6 more
europepmc   +1 more source

Identification and characterisation of calcitonin receptor isoforms expressed in glioblastoma derived glioma stem and U‐87 MG cells

FEBS Open Bio, EarlyView.
Glioblastoma cells express calcitonin receptor variants (CT receptor isoforms) that may help them survive stress. Using qPCR, transcript‐specific long‐read nanopore sequencing, immunofluorescence co‐localisation and comparative sequence analysis, this study identifies a novel alternatively spliced CALCR transcript that encodes the CTb receptor isoform ...
Pragya Gupta, Sebastian GB Furness, Tahereh Gharbi, Ric De Paoli‐Iseppi, Shweta S. Joshi, Michael Clark, David L. Hare, Peter Wookey   +7 more
wiley   +1 more source

A Cre-mediated copy number variant compromises the reliability of a <i>LoxP-STOP-LoxP</i>-<i>PLAG1</i> driven brain tumor model. [PDF]

Neurooncol Adv
Vaillant J, Pal S, Müller J, Wittmann A, Boakye-Yiadom A, Sievers P, Zimmer P, Schoof M, Schelb F, Hofmann N, Keck MK, Fabian T, Schüller U, Zuckermann M, Beroukhim R, Bandopadhayay P, Jones DTW, Kutscher LM.   +17 more
europepmc   +1 more source